Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5175 and Pf6N2E2_5176 are separated by 2 nucleotides Pf6N2E2_5176 and Pf6N2E2_5177 are separated by 45 nucleotides Pf6N2E2_5177 and Pf6N2E2_5178 are separated by 145 nucleotides
Pf6N2E2_5175 - Acetolactate synthase large subunit (EC 2.2.1.6), at 5,963,258 to 5,964,991
_5175
Pf6N2E2_5176 - Acetolactate synthase small subunit (EC 2.2.1.6), at 5,964,994 to 5,965,485
_5176
Pf6N2E2_5177 - Ketol-acid reductoisomerase (EC 1.1.1.86), at 5,965,531 to 5,966,547
_5177
Pf6N2E2_5178 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 5,966,693 to 5,967,538
_5178
Position (kb)
5965
5966
5967 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 5964.662 kb on + strand, within Pf6N2E2_5175 at 5964.663 kb on - strand, within Pf6N2E2_5175 at 5964.737 kb on + strand, within Pf6N2E2_5175 at 5964.738 kb on - strand, within Pf6N2E2_5175 at 5964.761 kb on + strand, within Pf6N2E2_5175 at 5964.761 kb on + strand, within Pf6N2E2_5175 at 5964.761 kb on + strand, within Pf6N2E2_5175 at 5964.761 kb on + strand, within Pf6N2E2_5175 at 5964.761 kb on + strand, within Pf6N2E2_5175 at 5964.762 kb on - strand, within Pf6N2E2_5175 at 5964.762 kb on - strand, within Pf6N2E2_5175 at 5964.803 kb on + strand, within Pf6N2E2_5175 at 5964.961 kb on + strand at 5964.961 kb on + strand at 5964.985 kb on + strand at 5964.985 kb on + strand at 5964.985 kb on + strand at 5964.986 kb on - strand at 5964.989 kb on + strand at 5964.989 kb on + strand at 5964.989 kb on + strand at 5964.989 kb on + strand at 5964.989 kb on + strand at 5964.990 kb on - strand at 5964.990 kb on - strand at 5964.990 kb on - strand at 5964.990 kb on - strand at 5965.005 kb on + strand at 5965.005 kb on + strand at 5965.006 kb on - strand at 5965.006 kb on - strand at 5965.006 kb on - strand at 5965.006 kb on - strand at 5965.052 kb on + strand, within Pf6N2E2_5176 at 5965.052 kb on + strand, within Pf6N2E2_5176 at 5965.052 kb on + strand, within Pf6N2E2_5176 at 5965.052 kb on + strand, within Pf6N2E2_5176 at 5965.052 kb on + strand, within Pf6N2E2_5176 at 5965.075 kb on + strand, within Pf6N2E2_5176 at 5965.075 kb on + strand, within Pf6N2E2_5176 at 5965.075 kb on + strand, within Pf6N2E2_5176 at 5965.076 kb on - strand, within Pf6N2E2_5176 at 5965.076 kb on - strand, within Pf6N2E2_5176 at 5965.076 kb on - strand, within Pf6N2E2_5176 at 5965.076 kb on - strand, within Pf6N2E2_5176 at 5965.076 kb on - strand, within Pf6N2E2_5176 at 5965.302 kb on + strand, within Pf6N2E2_5176 at 5965.303 kb on - strand, within Pf6N2E2_5176 at 5965.303 kb on - strand, within Pf6N2E2_5176 at 5965.303 kb on - strand, within Pf6N2E2_5176 at 5965.303 kb on - strand, within Pf6N2E2_5176 at 5965.305 kb on + strand, within Pf6N2E2_5176 at 5965.305 kb on + strand, within Pf6N2E2_5176 at 5965.312 kb on - strand, within Pf6N2E2_5176 at 5965.316 kb on - strand, within Pf6N2E2_5176 at 5965.316 kb on - strand, within Pf6N2E2_5176 at 5965.316 kb on - strand, within Pf6N2E2_5176 at 5965.351 kb on + strand, within Pf6N2E2_5176 at 5965.351 kb on + strand, within Pf6N2E2_5176 at 5965.351 kb on + strand, within Pf6N2E2_5176 at 5965.351 kb on + strand, within Pf6N2E2_5176 at 5965.351 kb on + strand, within Pf6N2E2_5176 at 5965.352 kb on - strand, within Pf6N2E2_5176 at 5965.352 kb on - strand, within Pf6N2E2_5176 at 5965.352 kb on - strand, within Pf6N2E2_5176 at 5965.353 kb on + strand, within Pf6N2E2_5176 at 5965.472 kb on + strand at 5965.472 kb on + strand at 5965.472 kb on + strand at 5965.472 kb on + strand at 5965.473 kb on - strand at 5965.473 kb on - strand at 5965.473 kb on - strand at 5965.483 kb on + strand at 5965.484 kb on - strand at 5965.484 kb on - strand at 5965.493 kb on + strand at 5965.543 kb on + strand at 5965.867 kb on + strand, within Pf6N2E2_5177 at 5965.867 kb on + strand, within Pf6N2E2_5177 at 5965.867 kb on + strand, within Pf6N2E2_5177 at 5965.867 kb on + strand, within Pf6N2E2_5177 at 5965.867 kb on + strand, within Pf6N2E2_5177 at 5965.868 kb on - strand, within Pf6N2E2_5177 at 5965.988 kb on - strand, within Pf6N2E2_5177 at 5965.988 kb on - strand, within Pf6N2E2_5177 at 5965.988 kb on - strand, within Pf6N2E2_5177 at 5966.030 kb on - strand, within Pf6N2E2_5177 at 5966.055 kb on + strand, within Pf6N2E2_5177 at 5966.182 kb on + strand, within Pf6N2E2_5177 at 5966.200 kb on + strand, within Pf6N2E2_5177 at 5966.200 kb on + strand, within Pf6N2E2_5177 at 5966.200 kb on + strand, within Pf6N2E2_5177 at 5966.200 kb on + strand, within Pf6N2E2_5177 at 5966.201 kb on - strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.245 kb on + strand, within Pf6N2E2_5177 at 5966.246 kb on - strand, within Pf6N2E2_5177 at 5966.246 kb on - strand, within Pf6N2E2_5177 at 5966.246 kb on - strand, within Pf6N2E2_5177 at 5966.272 kb on + strand, within Pf6N2E2_5177 at 5966.272 kb on + strand, within Pf6N2E2_5177 at 5966.273 kb on - strand, within Pf6N2E2_5177 at 5966.273 kb on - strand, within Pf6N2E2_5177 at 5966.273 kb on - strand, within Pf6N2E2_5177 at 5966.305 kb on + strand, within Pf6N2E2_5177 at 5966.386 kb on + strand, within Pf6N2E2_5177 at 5966.545 kb on + strand at 5966.545 kb on + strand at 5966.545 kb on + strand at 5966.546 kb on - strand at 5966.546 kb on - strand at 5966.546 kb on - strand at 5966.546 kb on - strand at 5966.546 kb on - strand at 5966.546 kb on - strand at 5966.615 kb on + strand at 5966.616 kb on - strand at 5966.685 kb on + strand at 5966.686 kb on - strand at 5966.686 kb on - strand at 5966.818 kb on - strand, within Pf6N2E2_5178 at 5967.140 kb on - strand, within Pf6N2E2_5178 at 5967.143 kb on + strand, within Pf6N2E2_5178 at 5967.144 kb on - strand, within Pf6N2E2_5178 at 5967.279 kb on - strand, within Pf6N2E2_5178 at 5967.362 kb on + strand, within Pf6N2E2_5178 at 5967.512 kb on + strand at 5967.513 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 5,964,662 + Pf6N2E2_5175 0.81 -3.3 5,964,663 - Pf6N2E2_5175 0.81 -1.1 5,964,737 + Pf6N2E2_5175 0.85 -2.4 5,964,738 - Pf6N2E2_5175 0.85 -1.9 5,964,761 + Pf6N2E2_5175 0.87 -3.3 5,964,761 + Pf6N2E2_5175 0.87 -1.7 5,964,761 + Pf6N2E2_5175 0.87 -2.3 5,964,761 + Pf6N2E2_5175 0.87 -0.1 5,964,761 + Pf6N2E2_5175 0.87 +0.1 5,964,762 - Pf6N2E2_5175 0.87 -0.8 5,964,762 - Pf6N2E2_5175 0.87 -3.1 5,964,803 + Pf6N2E2_5175 0.89 -2.1 5,964,961 + -1.8 5,964,961 + -2.7 5,964,985 + +0.5 5,964,985 + -0.2 5,964,985 + +0.8 5,964,986 - -3.4 5,964,989 + +1.4 5,964,989 + -1.9 5,964,989 + -1.1 5,964,989 + -2.0 5,964,989 + -1.3 5,964,990 - -1.3 5,964,990 - -0.3 5,964,990 - -0.4 5,964,990 - -1.8 5,965,005 + -1.9 5,965,005 + -2.5 5,965,006 - -1.8 5,965,006 - +0.8 5,965,006 - -1.1 5,965,006 - -0.9 5,965,052 + Pf6N2E2_5176 0.12 -2.5 5,965,052 + Pf6N2E2_5176 0.12 -2.6 5,965,052 + Pf6N2E2_5176 0.12 -2.8 5,965,052 + Pf6N2E2_5176 0.12 -1.8 5,965,052 + Pf6N2E2_5176 0.12 -2.4 5,965,075 + Pf6N2E2_5176 0.16 -3.0 5,965,075 + Pf6N2E2_5176 0.16 -3.4 5,965,075 + Pf6N2E2_5176 0.16 -2.2 5,965,076 - Pf6N2E2_5176 0.17 -0.9 5,965,076 - Pf6N2E2_5176 0.17 +2.4 5,965,076 - Pf6N2E2_5176 0.17 -3.3 5,965,076 - Pf6N2E2_5176 0.17 -2.3 5,965,076 - Pf6N2E2_5176 0.17 -2.9 5,965,302 + Pf6N2E2_5176 0.63 -1.2 5,965,303 - Pf6N2E2_5176 0.63 -2.8 5,965,303 - Pf6N2E2_5176 0.63 -1.7 5,965,303 - Pf6N2E2_5176 0.63 -2.5 5,965,303 - Pf6N2E2_5176 0.63 -3.3 5,965,305 + Pf6N2E2_5176 0.63 -0.6 5,965,305 + Pf6N2E2_5176 0.63 -2.2 5,965,312 - Pf6N2E2_5176 0.65 -1.8 5,965,316 - Pf6N2E2_5176 0.65 -2.7 5,965,316 - Pf6N2E2_5176 0.65 -3.2 5,965,316 - Pf6N2E2_5176 0.65 -0.2 5,965,351 + Pf6N2E2_5176 0.73 -2.5 5,965,351 + Pf6N2E2_5176 0.73 -1.1 5,965,351 + Pf6N2E2_5176 0.73 -3.4 5,965,351 + Pf6N2E2_5176 0.73 -3.1 5,965,351 + Pf6N2E2_5176 0.73 -2.3 5,965,352 - Pf6N2E2_5176 0.73 -3.0 5,965,352 - Pf6N2E2_5176 0.73 -1.6 5,965,352 - Pf6N2E2_5176 0.73 -0.8 5,965,353 + Pf6N2E2_5176 0.73 +0.8 5,965,472 + +1.9 5,965,472 + +2.2 5,965,472 + +2.0 5,965,472 + +2.2 5,965,473 - -2.6 5,965,473 - -1.2 5,965,473 - -2.6 5,965,483 + +0.1 5,965,484 - -2.7 5,965,484 - -2.2 5,965,493 + -1.4 5,965,543 + -0.9 5,965,867 + Pf6N2E2_5177 0.33 +0.4 5,965,867 + Pf6N2E2_5177 0.33 -0.5 5,965,867 + Pf6N2E2_5177 0.33 -3.9 5,965,867 + Pf6N2E2_5177 0.33 -2.7 5,965,867 + Pf6N2E2_5177 0.33 -2.5 5,965,868 - Pf6N2E2_5177 0.33 -2.6 5,965,988 - Pf6N2E2_5177 0.45 -1.9 5,965,988 - Pf6N2E2_5177 0.45 -2.8 5,965,988 - Pf6N2E2_5177 0.45 -2.6 5,966,030 - Pf6N2E2_5177 0.49 -2.6 5,966,055 + Pf6N2E2_5177 0.52 -3.7 5,966,182 + Pf6N2E2_5177 0.64 -1.6 5,966,200 + Pf6N2E2_5177 0.66 -1.8 5,966,200 + Pf6N2E2_5177 0.66 -2.9 5,966,200 + Pf6N2E2_5177 0.66 -1.9 5,966,200 + Pf6N2E2_5177 0.66 -3.0 5,966,201 - Pf6N2E2_5177 0.66 -1.9 5,966,245 + Pf6N2E2_5177 0.70 -0.8 5,966,245 + Pf6N2E2_5177 0.70 -1.3 5,966,245 + Pf6N2E2_5177 0.70 -2.6 5,966,245 + Pf6N2E2_5177 0.70 -2.0 5,966,245 + Pf6N2E2_5177 0.70 -2.0 5,966,245 + Pf6N2E2_5177 0.70 -3.7 5,966,245 + Pf6N2E2_5177 0.70 -2.1 5,966,245 + Pf6N2E2_5177 0.70 -3.4 5,966,246 - Pf6N2E2_5177 0.70 -1.4 5,966,246 - Pf6N2E2_5177 0.70 -1.9 5,966,246 - Pf6N2E2_5177 0.70 -3.7 5,966,272 + Pf6N2E2_5177 0.73 -1.6 5,966,272 + Pf6N2E2_5177 0.73 -2.3 5,966,273 - Pf6N2E2_5177 0.73 -2.5 5,966,273 - Pf6N2E2_5177 0.73 -1.9 5,966,273 - Pf6N2E2_5177 0.73 -2.4 5,966,305 + Pf6N2E2_5177 0.76 -2.7 5,966,386 + Pf6N2E2_5177 0.84 -3.3 5,966,545 + -0.2 5,966,545 + +0.9 5,966,545 + -0.2 5,966,546 - +0.1 5,966,546 - +0.9 5,966,546 - +0.9 5,966,546 - +1.2 5,966,546 - -0.5 5,966,546 - -0.6 5,966,615 + +0.0 5,966,616 - +1.6 5,966,685 + +2.4 5,966,686 - +3.6 5,966,686 - +3.0 5,966,818 - Pf6N2E2_5178 0.15 +3.8 5,967,140 - Pf6N2E2_5178 0.53 +4.8 5,967,143 + Pf6N2E2_5178 0.53 +3.0 5,967,144 - Pf6N2E2_5178 0.53 +3.5 5,967,279 - Pf6N2E2_5178 0.69 -0.2 5,967,362 + Pf6N2E2_5178 0.79 +2.5 5,967,512 + +1.0 5,967,513 - +3.7
Or see this region's nucleotide sequence