Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_515 and Pf6N2E2_516 are separated by 35 nucleotides Pf6N2E2_516 and Pf6N2E2_517 are separated by 148 nucleotides
Pf6N2E2_515 - malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) <small>(from data)</small>, at 584,020 to 585,522
_515
Pf6N2E2_516 - Inosose isomerase (EC 5.3.99.-), at 585,558 to 586,379
_516
Pf6N2E2_517 - Epi-inositol hydrolase (EC 3.7.1.-) <small>(from data)</small>, at 586,528 to 588,459
_517
Position (kb) 585
586
587 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.723 kb on - strand, within Pf6N2E2_515 at 584.723 kb on - strand, within Pf6N2E2_515 at 585.082 kb on - strand, within Pf6N2E2_515 at 585.082 kb on - strand, within Pf6N2E2_515 at 585.163 kb on + strand, within Pf6N2E2_515 at 585.163 kb on + strand, within Pf6N2E2_515 at 585.164 kb on - strand, within Pf6N2E2_515 at 585.164 kb on - strand, within Pf6N2E2_515 at 585.330 kb on - strand, within Pf6N2E2_515 at 585.415 kb on + strand at 585.440 kb on - strand at 585.448 kb on + strand at 585.520 kb on + strand at 585.521 kb on - strand at 585.521 kb on - strand at 585.553 kb on + strand at 585.553 kb on + strand at 585.553 kb on + strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.727 kb on + strand, within Pf6N2E2_516 at 585.728 kb on - strand, within Pf6N2E2_516 at 585.829 kb on - strand, within Pf6N2E2_516 at 585.969 kb on + strand, within Pf6N2E2_516 at 585.970 kb on - strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.064 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.441 kb on - strand at 586.441 kb on - strand at 586.585 kb on + strand at 586.585 kb on + strand at 586.586 kb on - strand at 586.780 kb on + strand, within Pf6N2E2_517 at 586.781 kb on - strand, within Pf6N2E2_517 at 586.781 kb on - strand, within Pf6N2E2_517 at 586.868 kb on + strand, within Pf6N2E2_517 at 586.869 kb on - strand at 586.869 kb on - strand, within Pf6N2E2_517 at 586.869 kb on - strand, within Pf6N2E2_517 at 586.885 kb on + strand, within Pf6N2E2_517 at 586.982 kb on - strand, within Pf6N2E2_517 at 587.111 kb on - strand, within Pf6N2E2_517 at 587.126 kb on - strand, within Pf6N2E2_517 at 587.183 kb on + strand, within Pf6N2E2_517 at 587.183 kb on + strand, within Pf6N2E2_517
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 584,722 + Pf6N2E2_515 0.47 +1.3 584,722 + Pf6N2E2_515 0.47 -0.4 584,722 + Pf6N2E2_515 0.47 -0.8 584,722 + Pf6N2E2_515 0.47 -0.4 584,723 - Pf6N2E2_515 0.47 -1.7 584,723 - Pf6N2E2_515 0.47 -0.5 585,082 - Pf6N2E2_515 0.71 -0.9 585,082 - Pf6N2E2_515 0.71 +0.0 585,163 + Pf6N2E2_515 0.76 -2.2 585,163 + Pf6N2E2_515 0.76 -2.2 585,164 - Pf6N2E2_515 0.76 -0.6 585,164 - Pf6N2E2_515 0.76 +1.2 585,330 - Pf6N2E2_515 0.87 -1.4 585,415 + -1.2 585,440 - +0.8 585,448 + -2.1 585,520 + -2.1 585,521 - +0.8 585,521 - +1.5 585,553 + -1.0 585,553 + -1.5 585,553 + -0.3 585,554 - -1.2 585,554 - -1.0 585,554 - -0.7 585,554 - -1.8 585,554 - +2.0 585,554 - +0.2 585,554 - +0.2 585,554 - +0.1 585,727 + Pf6N2E2_516 0.21 -0.9 585,728 - Pf6N2E2_516 0.21 -1.4 585,829 - Pf6N2E2_516 0.33 -2.2 585,969 + Pf6N2E2_516 0.50 -0.1 585,970 - Pf6N2E2_516 0.50 +0.2 586,063 + Pf6N2E2_516 0.61 -1.2 586,063 + Pf6N2E2_516 0.61 -0.2 586,063 + Pf6N2E2_516 0.61 -2.2 586,064 - Pf6N2E2_516 0.62 +0.6 586,279 - Pf6N2E2_516 0.88 -0.6 586,279 - Pf6N2E2_516 0.88 -0.2 586,279 - Pf6N2E2_516 0.88 -0.4 586,441 - +1.5 586,441 - -0.5 586,585 + -1.5 586,585 + +0.5 586,586 - +0.2 586,780 + Pf6N2E2_517 0.13 +0.7 586,781 - Pf6N2E2_517 0.13 +0.5 586,781 - Pf6N2E2_517 0.13 -0.5 586,868 + Pf6N2E2_517 0.18 +1.2 586,869 - +0.8 586,869 - Pf6N2E2_517 0.18 +0.4 586,869 - Pf6N2E2_517 0.18 -1.6 586,885 + Pf6N2E2_517 0.18 -0.4 586,982 - Pf6N2E2_517 0.23 -2.7 587,111 - Pf6N2E2_517 0.30 +2.8 587,126 - Pf6N2E2_517 0.31 +0.2 587,183 + Pf6N2E2_517 0.34 +1.3 587,183 + Pf6N2E2_517 0.34 +1.6
Or see this region's nucleotide sequence