Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5155 and Pf6N2E2_5156 are separated by 718 nucleotides Pf6N2E2_5156 and Pf6N2E2_5157 are separated by 22 nucleotides
Pf6N2E2_5155 - Poly(A) polymerase (EC 2.7.7.19), at 5,941,690 to 5,943,087
_5155
Pf6N2E2_5156 - Two-component sensor CbrB: intrcellular carbon:nitrogen balance, at 5,943,806 to 5,945,239
_5156
Pf6N2E2_5157 - Two-component sensor CbrA: intrcellular carbon:nitrogen balance, at 5,945,262 to 5,948,210
_5157
Position (kb)
5943
5944
5945
5946 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7
8 at 5942.830 kb on + strand, within Pf6N2E2_5155 at 5942.919 kb on - strand, within Pf6N2E2_5155 at 5942.919 kb on - strand, within Pf6N2E2_5155 at 5943.110 kb on - strand at 5943.154 kb on + strand at 5943.154 kb on + strand at 5943.155 kb on - strand at 5943.161 kb on + strand at 5943.161 kb on + strand at 5943.223 kb on + strand at 5943.224 kb on - strand at 5943.282 kb on + strand at 5943.283 kb on - strand at 5943.287 kb on - strand at 5943.302 kb on + strand at 5943.306 kb on - strand at 5943.468 kb on - strand at 5943.468 kb on - strand at 5943.503 kb on + strand at 5943.504 kb on - strand at 5943.504 kb on - strand at 5943.504 kb on - strand at 5943.504 kb on - strand at 5943.529 kb on + strand at 5943.530 kb on - strand at 5943.557 kb on - strand at 5943.581 kb on - strand at 5943.586 kb on + strand at 5943.608 kb on + strand at 5943.619 kb on + strand at 5943.628 kb on + strand at 5943.628 kb on + strand at 5943.629 kb on - strand at 5943.686 kb on + strand at 5943.686 kb on + strand at 5943.687 kb on - strand at 5943.688 kb on + strand at 5943.689 kb on - strand at 5943.732 kb on + strand at 5943.733 kb on - strand at 5943.733 kb on - strand at 5943.750 kb on - strand at 5943.768 kb on - strand at 5943.768 kb on - strand at 5943.955 kb on - strand, within Pf6N2E2_5156 at 5944.326 kb on + strand, within Pf6N2E2_5156 at 5944.326 kb on + strand, within Pf6N2E2_5156 at 5944.327 kb on - strand, within Pf6N2E2_5156 at 5944.767 kb on + strand, within Pf6N2E2_5156 at 5944.816 kb on + strand, within Pf6N2E2_5156 at 5944.822 kb on + strand, within Pf6N2E2_5156 at 5944.956 kb on + strand, within Pf6N2E2_5156 at 5944.957 kb on - strand, within Pf6N2E2_5156 at 5944.957 kb on - strand, within Pf6N2E2_5156 at 5945.334 kb on + strand at 5945.334 kb on + strand at 5945.335 kb on - strand at 5945.335 kb on - strand at 5945.390 kb on - strand at 5946.156 kb on + strand, within Pf6N2E2_5157
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 5,942,830 + Pf6N2E2_5155 0.82 +4.3 5,942,919 - Pf6N2E2_5155 0.88 +5.5 5,942,919 - Pf6N2E2_5155 0.88 +5.9 5,943,110 - +1.2 5,943,154 + +2.4 5,943,154 + +1.7 5,943,155 - +0.2 5,943,161 + +0.2 5,943,161 + -0.3 5,943,223 + +0.6 5,943,224 - +1.1 5,943,282 + +2.4 5,943,283 - +4.0 5,943,287 - +3.7 5,943,302 + +3.5 5,943,306 - +3.9 5,943,468 - +4.9 5,943,468 - +5.2 5,943,503 + +6.2 5,943,504 - +5.4 5,943,504 - +6.0 5,943,504 - +4.8 5,943,504 - +5.2 5,943,529 + +4.9 5,943,530 - +4.9 5,943,557 - +5.7 5,943,581 - +4.3 5,943,586 + +7.8 5,943,608 + +6.2 5,943,619 + +6.3 5,943,628 + +6.6 5,943,628 + +6.5 5,943,629 - +2.9 5,943,686 + +6.4 5,943,686 + +6.3 5,943,687 - +4.8 5,943,688 + +6.6 5,943,689 - +3.5 5,943,732 + +6.3 5,943,733 - +4.3 5,943,733 - +4.7 5,943,750 - +5.3 5,943,768 - +0.9 5,943,768 - +1.5 5,943,955 - Pf6N2E2_5156 0.10 -0.9 5,944,326 + Pf6N2E2_5156 0.36 -1.0 5,944,326 + Pf6N2E2_5156 0.36 +0.1 5,944,327 - Pf6N2E2_5156 0.36 -1.6 5,944,767 + Pf6N2E2_5156 0.67 -0.4 5,944,816 + Pf6N2E2_5156 0.70 -1.3 5,944,822 + Pf6N2E2_5156 0.71 +0.8 5,944,956 + Pf6N2E2_5156 0.80 -1.2 5,944,957 - Pf6N2E2_5156 0.80 -0.6 5,944,957 - Pf6N2E2_5156 0.80 +0.4 5,945,334 + +0.8 5,945,334 + -0.6 5,945,335 - +1.5 5,945,335 - +0.0 5,945,390 - +1.1 5,946,156 + Pf6N2E2_5157 0.30 +1.1
Or see this region's nucleotide sequence