Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4539 and Pf6N2E2_4540 are separated by 419 nucleotides Pf6N2E2_4540 and Pf6N2E2_4541 are separated by 124 nucleotides Pf6N2E2_4541 and Pf6N2E2_4542 are separated by 206 nucleotides Pf6N2E2_4542 and Pf6N2E2_4543 are separated by 6 nucleotides Pf6N2E2_4543 and Pf6N2E2_4544 overlap by 4 nucleotides
Pf6N2E2_4539 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 5,272,146 to 5,273,246
_4539
Pf6N2E2_4540 - hypothetical protein, at 5,273,666 to 5,273,881
_4540
Pf6N2E2_4541 - hypothetical protein, at 5,274,006 to 5,274,455
_4541
Pf6N2E2_4542 - negative regulator of hrp expression HrpV, at 5,274,662 to 5,275,039
_4542
Pf6N2E2_4543 - hypothetical protein, at 5,275,046 to 5,275,255
_4543
Pf6N2E2_4544 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 5,275,252 to 5,277,369
_4544
Position (kb) 5274
5275 Strain fitness (log2 ratio) -2
-1
0
1 at 5273.113 kb on - strand, within Pf6N2E2_4539 at 5273.133 kb on + strand, within Pf6N2E2_4539 at 5273.173 kb on - strand at 5273.266 kb on + strand at 5273.394 kb on + strand at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.762 kb on - strand, within Pf6N2E2_4540 at 5273.897 kb on + strand at 5273.897 kb on + strand at 5273.897 kb on + strand at 5273.898 kb on - strand at 5273.999 kb on + strand at 5273.999 kb on + strand at 5274.000 kb on - strand at 5274.362 kb on - strand, within Pf6N2E2_4541 at 5274.471 kb on + strand at 5274.471 kb on + strand at 5274.471 kb on + strand at 5274.472 kb on - strand at 5274.666 kb on + strand at 5274.666 kb on + strand at 5274.667 kb on - strand at 5274.678 kb on + strand at 5274.678 kb on + strand at 5274.679 kb on - strand at 5274.806 kb on + strand, within Pf6N2E2_4542 at 5274.806 kb on + strand, within Pf6N2E2_4542 at 5274.807 kb on - strand, within Pf6N2E2_4542 at 5274.807 kb on - strand, within Pf6N2E2_4542 at 5274.822 kb on + strand, within Pf6N2E2_4542 at 5274.893 kb on + strand, within Pf6N2E2_4542 at 5274.893 kb on + strand, within Pf6N2E2_4542 at 5274.893 kb on + strand, within Pf6N2E2_4542 at 5274.894 kb on - strand, within Pf6N2E2_4542 at 5274.894 kb on - strand, within Pf6N2E2_4542 at 5274.899 kb on + strand, within Pf6N2E2_4542 at 5274.957 kb on - strand, within Pf6N2E2_4542 at 5274.957 kb on - strand, within Pf6N2E2_4542 at 5274.957 kb on - strand, within Pf6N2E2_4542 at 5274.967 kb on - strand, within Pf6N2E2_4542 at 5274.967 kb on - strand, within Pf6N2E2_4542 at 5274.967 kb on - strand, within Pf6N2E2_4542 at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.039 kb on + strand at 5275.039 kb on + strand at 5275.039 kb on + strand at 5275.040 kb on - strand at 5275.040 kb on - strand at 5275.069 kb on + strand, within Pf6N2E2_4543 at 5275.070 kb on - strand, within Pf6N2E2_4543 at 5275.226 kb on + strand, within Pf6N2E2_4543 at 5275.226 kb on + strand, within Pf6N2E2_4543
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 5,273,113 - Pf6N2E2_4539 0.88 +0.3 5,273,133 + Pf6N2E2_4539 0.90 -1.0 5,273,173 - -1.8 5,273,266 + +0.4 5,273,394 + -0.3 5,273,761 + Pf6N2E2_4540 0.44 +1.2 5,273,761 + Pf6N2E2_4540 0.44 -0.2 5,273,761 + Pf6N2E2_4540 0.44 +0.2 5,273,762 - Pf6N2E2_4540 0.44 +0.8 5,273,897 + -1.9 5,273,897 + +0.5 5,273,897 + -1.2 5,273,898 - +0.6 5,273,999 + +0.4 5,273,999 + +1.1 5,274,000 - +0.4 5,274,362 - Pf6N2E2_4541 0.79 -0.7 5,274,471 + -0.6 5,274,471 + -2.0 5,274,471 + +1.2 5,274,472 - -1.9 5,274,666 + +0.5 5,274,666 + +0.9 5,274,667 - -0.7 5,274,678 + +0.1 5,274,678 + +0.8 5,274,679 - -0.4 5,274,806 + Pf6N2E2_4542 0.38 +0.4 5,274,806 + Pf6N2E2_4542 0.38 +1.0 5,274,807 - Pf6N2E2_4542 0.38 -0.5 5,274,807 - Pf6N2E2_4542 0.38 -0.3 5,274,822 + Pf6N2E2_4542 0.42 +0.8 5,274,893 + Pf6N2E2_4542 0.61 +0.0 5,274,893 + Pf6N2E2_4542 0.61 -0.4 5,274,893 + Pf6N2E2_4542 0.61 +0.5 5,274,894 - Pf6N2E2_4542 0.61 -1.3 5,274,894 - Pf6N2E2_4542 0.61 -2.3 5,274,899 + Pf6N2E2_4542 0.63 +0.1 5,274,957 - Pf6N2E2_4542 0.78 +0.6 5,274,957 - Pf6N2E2_4542 0.78 +0.4 5,274,957 - Pf6N2E2_4542 0.78 +0.3 5,274,967 - Pf6N2E2_4542 0.81 -0.3 5,274,967 - Pf6N2E2_4542 0.81 -1.1 5,274,967 - Pf6N2E2_4542 0.81 -0.4 5,275,017 - -2.6 5,275,017 - +0.1 5,275,017 - -0.4 5,275,017 - +1.1 5,275,017 - +1.1 5,275,039 + -0.4 5,275,039 + +0.2 5,275,039 + -0.7 5,275,040 - -2.3 5,275,040 - -0.4 5,275,069 + Pf6N2E2_4543 0.11 +0.6 5,275,070 - Pf6N2E2_4543 0.11 -0.4 5,275,226 + Pf6N2E2_4543 0.86 +0.9 5,275,226 + Pf6N2E2_4543 0.86 -1.4
Or see this region's nucleotide sequence