Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4508 and Pf6N2E2_4509 are separated by 310 nucleotides Pf6N2E2_4509 and Pf6N2E2_4510 are separated by 383 nucleotides
Pf6N2E2_4508 - Transcriptional regulator, TetR family, at 5,239,289 to 5,239,921
_4508
Pf6N2E2_4509 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data) , at 5,240,232 to 5,241,503
_4509
Pf6N2E2_4510 - Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94) (from data) , at 5,241,887 to 5,242,663
_4510
Position (kb)
5240
5241
5242 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5239.311 kb on + strand at 5239.311 kb on + strand at 5239.312 kb on - strand at 5239.323 kb on + strand at 5239.323 kb on + strand at 5239.323 kb on + strand at 5239.324 kb on - strand at 5239.324 kb on - strand at 5239.335 kb on + strand at 5239.335 kb on + strand at 5239.335 kb on + strand at 5239.336 kb on - strand at 5239.336 kb on - strand at 5239.398 kb on - strand, within Pf6N2E2_4508 at 5239.410 kb on + strand, within Pf6N2E2_4508 at 5239.605 kb on + strand, within Pf6N2E2_4508 at 5239.605 kb on + strand, within Pf6N2E2_4508 at 5239.605 kb on + strand, within Pf6N2E2_4508 at 5239.606 kb on - strand, within Pf6N2E2_4508 at 5239.606 kb on - strand, within Pf6N2E2_4508 at 5239.803 kb on + strand, within Pf6N2E2_4508 at 5239.804 kb on - strand, within Pf6N2E2_4508 at 5239.952 kb on + strand at 5239.952 kb on + strand at 5239.953 kb on - strand at 5239.953 kb on - strand at 5239.953 kb on - strand at 5239.953 kb on - strand at 5239.953 kb on - strand at 5239.976 kb on + strand at 5239.976 kb on + strand at 5239.976 kb on + strand at 5239.977 kb on - strand at 5239.980 kb on + strand at 5239.980 kb on + strand at 5239.980 kb on + strand at 5239.980 kb on + strand at 5239.980 kb on + strand at 5239.981 kb on - strand at 5239.981 kb on - strand at 5239.981 kb on - strand at 5239.981 kb on - strand at 5239.981 kb on - strand at 5240.021 kb on - strand at 5240.021 kb on - strand at 5240.021 kb on - strand at 5240.021 kb on - strand at 5240.039 kb on + strand at 5240.040 kb on - strand at 5240.040 kb on - strand at 5240.067 kb on - strand at 5240.099 kb on + strand at 5240.099 kb on + strand at 5240.099 kb on + strand at 5240.099 kb on + strand at 5240.099 kb on + strand at 5240.100 kb on - strand at 5240.100 kb on - strand at 5240.100 kb on - strand at 5240.100 kb on - strand at 5240.127 kb on - strand at 5240.179 kb on + strand at 5240.180 kb on - strand at 5240.221 kb on - strand at 5240.233 kb on + strand at 5240.234 kb on - strand at 5240.234 kb on - strand at 5240.240 kb on + strand at 5240.241 kb on - strand at 5240.241 kb on - strand at 5240.248 kb on + strand at 5240.248 kb on + strand at 5240.248 kb on + strand at 5240.257 kb on + strand at 5240.258 kb on - strand at 5240.258 kb on - strand at 5240.258 kb on - strand at 5240.321 kb on + strand at 5240.321 kb on + strand at 5240.322 kb on - strand at 5240.322 kb on - strand at 5240.322 kb on - strand at 5240.329 kb on + strand at 5240.344 kb on + strand at 5240.344 kb on + strand at 5240.345 kb on - strand at 5240.345 kb on - strand at 5240.345 kb on - strand at 5240.425 kb on + strand, within Pf6N2E2_4509 at 5240.425 kb on + strand, within Pf6N2E2_4509 at 5240.425 kb on + strand, within Pf6N2E2_4509 at 5240.426 kb on - strand, within Pf6N2E2_4509 at 5240.426 kb on - strand, within Pf6N2E2_4509 at 5240.513 kb on - strand, within Pf6N2E2_4509 at 5240.632 kb on + strand, within Pf6N2E2_4509 at 5240.633 kb on - strand, within Pf6N2E2_4509 at 5240.633 kb on - strand, within Pf6N2E2_4509 at 5240.657 kb on - strand, within Pf6N2E2_4509 at 5240.657 kb on - strand, within Pf6N2E2_4509 at 5240.784 kb on - strand, within Pf6N2E2_4509 at 5240.784 kb on - strand, within Pf6N2E2_4509 at 5240.872 kb on + strand, within Pf6N2E2_4509 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5241.049 kb on + strand, within Pf6N2E2_4509 at 5241.049 kb on + strand, within Pf6N2E2_4509 at 5241.050 kb on - strand, within Pf6N2E2_4509 at 5241.050 kb on - strand, within Pf6N2E2_4509 at 5241.074 kb on - strand, within Pf6N2E2_4509 at 5241.074 kb on - strand, within Pf6N2E2_4509 at 5241.160 kb on + strand, within Pf6N2E2_4509 at 5241.160 kb on + strand, within Pf6N2E2_4509 at 5241.160 kb on + strand, within Pf6N2E2_4509 at 5241.161 kb on - strand, within Pf6N2E2_4509 at 5241.402 kb on - strand at 5241.426 kb on + strand at 5241.426 kb on + strand at 5241.427 kb on - strand at 5241.457 kb on + strand at 5241.483 kb on + strand at 5241.484 kb on - strand at 5241.484 kb on - strand at 5241.512 kb on + strand at 5241.535 kb on + strand at 5241.536 kb on - strand at 5241.536 kb on - strand at 5241.579 kb on - strand at 5241.628 kb on + strand at 5241.634 kb on + strand at 5241.803 kb on - strand at 5241.825 kb on - strand at 5241.842 kb on + strand at 5241.870 kb on + strand at 5241.870 kb on + strand at 5241.870 kb on + strand at 5241.871 kb on - strand at 5241.873 kb on + strand at 5241.947 kb on + strand at 5241.947 kb on + strand at 5241.968 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.099 kb on - strand, within Pf6N2E2_4510 at 5242.248 kb on + strand, within Pf6N2E2_4510 at 5242.248 kb on + strand, within Pf6N2E2_4510 at 5242.249 kb on - strand, within Pf6N2E2_4510 at 5242.249 kb on - strand, within Pf6N2E2_4510 at 5242.319 kb on - strand, within Pf6N2E2_4510 at 5242.319 kb on - strand, within Pf6N2E2_4510 at 5242.340 kb on + strand, within Pf6N2E2_4510 at 5242.364 kb on - strand, within Pf6N2E2_4510 at 5242.380 kb on + strand, within Pf6N2E2_4510 at 5242.380 kb on + strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.411 kb on - strand, within Pf6N2E2_4510 at 5242.411 kb on - strand, within Pf6N2E2_4510
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 5,239,311 + +0.6 5,239,311 + +0.6 5,239,312 - +1.6 5,239,323 + -0.1 5,239,323 + -0.2 5,239,323 + -0.4 5,239,324 - -0.2 5,239,324 - +0.2 5,239,335 + +0.2 5,239,335 + -0.2 5,239,335 + -1.5 5,239,336 - -0.9 5,239,336 - -1.4 5,239,398 - Pf6N2E2_4508 0.17 -1.0 5,239,410 + Pf6N2E2_4508 0.19 -0.5 5,239,605 + Pf6N2E2_4508 0.50 +1.7 5,239,605 + Pf6N2E2_4508 0.50 -2.0 5,239,605 + Pf6N2E2_4508 0.50 +0.7 5,239,606 - Pf6N2E2_4508 0.50 +1.4 5,239,606 - Pf6N2E2_4508 0.50 +2.2 5,239,803 + Pf6N2E2_4508 0.81 +1.1 5,239,804 - Pf6N2E2_4508 0.81 -0.9 5,239,952 + -0.2 5,239,952 + +1.0 5,239,953 - -0.9 5,239,953 - -0.1 5,239,953 - +0.8 5,239,953 - +0.6 5,239,953 - +0.6 5,239,976 + +1.0 5,239,976 + -0.9 5,239,976 + +0.8 5,239,977 - -1.4 5,239,980 + +0.6 5,239,980 + -1.3 5,239,980 + +0.9 5,239,980 + -0.2 5,239,980 + +0.0 5,239,981 - -0.3 5,239,981 - -0.3 5,239,981 - -0.1 5,239,981 - -0.2 5,239,981 - +0.3 5,240,021 - -1.0 5,240,021 - -1.6 5,240,021 - +2.2 5,240,021 - -0.4 5,240,039 + -0.7 5,240,040 - -1.1 5,240,040 - +0.7 5,240,067 - -0.2 5,240,099 + -0.0 5,240,099 + -0.9 5,240,099 + -2.1 5,240,099 + -1.1 5,240,099 + +0.4 5,240,100 - +0.6 5,240,100 - -0.2 5,240,100 - +1.4 5,240,100 - +0.6 5,240,127 - -0.4 5,240,179 + -0.5 5,240,180 - +0.5 5,240,221 - +0.4 5,240,233 + -0.6 5,240,234 - -2.2 5,240,234 - -1.5 5,240,240 + +0.2 5,240,241 - -0.3 5,240,241 - -1.7 5,240,248 + -1.1 5,240,248 + -1.4 5,240,248 + +0.0 5,240,257 + -0.5 5,240,258 - -0.1 5,240,258 - +0.0 5,240,258 - -0.9 5,240,321 + -1.1 5,240,321 + -1.7 5,240,322 - -0.2 5,240,322 - -1.4 5,240,322 - -0.1 5,240,329 + -0.1 5,240,344 + -2.4 5,240,344 + +0.1 5,240,345 - -1.6 5,240,345 - +0.1 5,240,345 - -0.3 5,240,425 + Pf6N2E2_4509 0.15 +0.2 5,240,425 + Pf6N2E2_4509 0.15 -0.4 5,240,425 + Pf6N2E2_4509 0.15 -1.1 5,240,426 - Pf6N2E2_4509 0.15 -1.0 5,240,426 - Pf6N2E2_4509 0.15 +0.2 5,240,513 - Pf6N2E2_4509 0.22 -1.4 5,240,632 + Pf6N2E2_4509 0.31 -0.7 5,240,633 - Pf6N2E2_4509 0.32 -1.1 5,240,633 - Pf6N2E2_4509 0.32 +0.9 5,240,657 - Pf6N2E2_4509 0.33 -1.0 5,240,657 - Pf6N2E2_4509 0.33 -0.3 5,240,784 - Pf6N2E2_4509 0.43 +0.4 5,240,784 - Pf6N2E2_4509 0.43 -2.3 5,240,872 + Pf6N2E2_4509 0.50 +0.1 5,240,911 + Pf6N2E2_4509 0.53 -1.5 5,240,911 + Pf6N2E2_4509 0.53 +1.2 5,240,911 + Pf6N2E2_4509 0.53 -0.6 5,241,049 + Pf6N2E2_4509 0.64 -0.8 5,241,049 + Pf6N2E2_4509 0.64 +0.5 5,241,050 - Pf6N2E2_4509 0.64 -0.9 5,241,050 - Pf6N2E2_4509 0.64 -2.4 5,241,074 - Pf6N2E2_4509 0.66 -0.6 5,241,074 - Pf6N2E2_4509 0.66 -0.7 5,241,160 + Pf6N2E2_4509 0.73 -1.3 5,241,160 + Pf6N2E2_4509 0.73 -1.1 5,241,160 + Pf6N2E2_4509 0.73 -0.5 5,241,161 - Pf6N2E2_4509 0.73 -0.6 5,241,402 - +1.2 5,241,426 + -0.2 5,241,426 + -2.4 5,241,427 - +0.6 5,241,457 + -0.6 5,241,483 + +0.2 5,241,484 - -1.0 5,241,484 - +1.5 5,241,512 + -0.8 5,241,535 + +0.3 5,241,536 - -1.2 5,241,536 - -1.2 5,241,579 - +1.5 5,241,628 + +0.7 5,241,634 + -0.7 5,241,803 - +1.3 5,241,825 - +0.9 5,241,842 + +0.2 5,241,870 + -0.3 5,241,870 + +0.1 5,241,870 + +0.2 5,241,871 - +0.3 5,241,873 + +0.2 5,241,947 + +0.5 5,241,947 + -0.5 5,241,968 + Pf6N2E2_4510 0.10 -0.8 5,242,098 + Pf6N2E2_4510 0.27 +0.9 5,242,098 + Pf6N2E2_4510 0.27 -0.9 5,242,098 + Pf6N2E2_4510 0.27 +0.2 5,242,098 + Pf6N2E2_4510 0.27 -1.4 5,242,098 + Pf6N2E2_4510 0.27 +1.0 5,242,099 - Pf6N2E2_4510 0.27 +0.8 5,242,248 + Pf6N2E2_4510 0.46 +0.4 5,242,248 + Pf6N2E2_4510 0.46 -0.2 5,242,249 - Pf6N2E2_4510 0.47 +0.8 5,242,249 - Pf6N2E2_4510 0.47 -0.6 5,242,319 - Pf6N2E2_4510 0.56 +0.7 5,242,319 - Pf6N2E2_4510 0.56 +0.9 5,242,340 + Pf6N2E2_4510 0.58 +1.2 5,242,364 - Pf6N2E2_4510 0.61 +1.0 5,242,380 + Pf6N2E2_4510 0.63 +0.1 5,242,380 + Pf6N2E2_4510 0.63 +2.8 5,242,381 - Pf6N2E2_4510 0.64 -0.2 5,242,381 - Pf6N2E2_4510 0.64 +0.2 5,242,381 - Pf6N2E2_4510 0.64 -0.4 5,242,381 - Pf6N2E2_4510 0.64 -0.3 5,242,381 - Pf6N2E2_4510 0.64 -0.3 5,242,410 + Pf6N2E2_4510 0.67 +1.3 5,242,410 + Pf6N2E2_4510 0.67 -0.2 5,242,410 + Pf6N2E2_4510 0.67 -0.6 5,242,410 + Pf6N2E2_4510 0.67 +0.2 5,242,411 - Pf6N2E2_4510 0.67 +0.6 5,242,411 - Pf6N2E2_4510 0.67 -0.1
Or see this region's nucleotide sequence