Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4329 and Pf6N2E2_4330 overlap by 4 nucleotides Pf6N2E2_4330 and Pf6N2E2_4331 are separated by 47 nucleotides Pf6N2E2_4331 and Pf6N2E2_4332 overlap by 4 nucleotides Pf6N2E2_4332 and Pf6N2E2_4333 are separated by 81 nucleotides
Pf6N2E2_4329 - Protein ImpG/VasA, at 5,052,119 to 5,053,978
_4329
Pf6N2E2_4330 - Uncharacterized protein ImpF, at 5,053,975 to 5,054,433
_4330
Pf6N2E2_4331 - hypothetical protein, at 5,054,481 to 5,054,930
_4331
Pf6N2E2_4332 - hypothetical protein, at 5,054,927 to 5,055,382
_4332
Pf6N2E2_4333 - Uncharacterized protein ImpD, at 5,055,464 to 5,055,952
_4333
Position (kb)
5054
5055 Strain fitness (log2 ratio)
-2
-1
0
1 at 5053.536 kb on + strand, within Pf6N2E2_4329 at 5053.731 kb on + strand, within Pf6N2E2_4329 at 5053.732 kb on - strand, within Pf6N2E2_4329 at 5053.846 kb on - strand at 5053.953 kb on + strand at 5053.953 kb on + strand at 5053.953 kb on + strand at 5053.954 kb on - strand at 5053.954 kb on - strand at 5054.503 kb on + strand at 5054.586 kb on + strand, within Pf6N2E2_4331 at 5054.587 kb on - strand, within Pf6N2E2_4331 at 5054.657 kb on + strand, within Pf6N2E2_4331 at 5054.657 kb on + strand, within Pf6N2E2_4331 at 5054.657 kb on + strand, within Pf6N2E2_4331 at 5054.658 kb on - strand, within Pf6N2E2_4331 at 5054.907 kb on + strand at 5054.907 kb on + strand at 5054.908 kb on - strand at 5054.908 kb on - strand at 5054.908 kb on - strand at 5054.930 kb on + strand at 5054.930 kb on + strand at 5054.930 kb on + strand at 5054.931 kb on - strand at 5054.931 kb on - strand at 5054.931 kb on - strand at 5055.219 kb on + strand, within Pf6N2E2_4332 at 5055.219 kb on + strand, within Pf6N2E2_4332 at 5055.220 kb on - strand, within Pf6N2E2_4332 at 5055.220 kb on - strand, within Pf6N2E2_4332 at 5055.253 kb on + strand, within Pf6N2E2_4332 at 5055.253 kb on + strand, within Pf6N2E2_4332 at 5055.254 kb on - strand, within Pf6N2E2_4332 at 5055.325 kb on - strand, within Pf6N2E2_4332 at 5055.345 kb on + strand at 5055.345 kb on + strand at 5055.345 kb on + strand at 5055.345 kb on + strand at 5055.346 kb on - strand at 5055.391 kb on + strand at 5055.391 kb on + strand at 5055.391 kb on + strand at 5055.392 kb on - strand at 5055.392 kb on - strand at 5055.465 kb on + strand at 5055.465 kb on + strand at 5055.466 kb on - strand at 5055.631 kb on + strand, within Pf6N2E2_4333 at 5055.631 kb on + strand, within Pf6N2E2_4333 at 5055.632 kb on - strand, within Pf6N2E2_4333 at 5055.663 kb on + strand, within Pf6N2E2_4333 at 5055.663 kb on + strand, within Pf6N2E2_4333 at 5055.663 kb on + strand, within Pf6N2E2_4333 at 5055.664 kb on - strand, within Pf6N2E2_4333 at 5055.664 kb on - strand, within Pf6N2E2_4333 at 5055.771 kb on + strand, within Pf6N2E2_4333 at 5055.771 kb on + strand, within Pf6N2E2_4333 at 5055.771 kb on + strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 5,053,536 + Pf6N2E2_4329 0.76 +0.6 5,053,731 + Pf6N2E2_4329 0.87 -1.2 5,053,732 - Pf6N2E2_4329 0.87 +1.3 5,053,846 - +0.6 5,053,953 + +0.6 5,053,953 + +1.0 5,053,953 + -0.1 5,053,954 - +0.7 5,053,954 - +0.8 5,054,503 + +0.8 5,054,586 + Pf6N2E2_4331 0.23 -0.8 5,054,587 - Pf6N2E2_4331 0.24 -1.8 5,054,657 + Pf6N2E2_4331 0.39 +0.3 5,054,657 + Pf6N2E2_4331 0.39 -0.4 5,054,657 + Pf6N2E2_4331 0.39 -1.6 5,054,658 - Pf6N2E2_4331 0.39 -0.2 5,054,907 + +0.3 5,054,907 + +0.5 5,054,908 - -0.5 5,054,908 - -1.4 5,054,908 - -2.4 5,054,930 + -0.2 5,054,930 + +0.5 5,054,930 + +1.1 5,054,931 - +1.2 5,054,931 - +0.4 5,054,931 - +0.9 5,055,219 + Pf6N2E2_4332 0.64 +0.9 5,055,219 + Pf6N2E2_4332 0.64 +0.6 5,055,220 - Pf6N2E2_4332 0.64 +0.1 5,055,220 - Pf6N2E2_4332 0.64 -0.3 5,055,253 + Pf6N2E2_4332 0.71 +0.5 5,055,253 + Pf6N2E2_4332 0.71 +1.2 5,055,254 - Pf6N2E2_4332 0.72 -0.2 5,055,325 - Pf6N2E2_4332 0.87 +0.8 5,055,345 + +0.7 5,055,345 + +0.3 5,055,345 + +0.4 5,055,345 + -0.8 5,055,346 - -0.4 5,055,391 + +0.9 5,055,391 + -1.1 5,055,391 + +0.4 5,055,392 - -0.6 5,055,392 - +0.8 5,055,465 + +1.1 5,055,465 + +1.7 5,055,466 - -0.3 5,055,631 + Pf6N2E2_4333 0.34 +0.6 5,055,631 + Pf6N2E2_4333 0.34 +0.4 5,055,632 - Pf6N2E2_4333 0.34 +0.1 5,055,663 + Pf6N2E2_4333 0.41 +0.4 5,055,663 + Pf6N2E2_4333 0.41 -0.2 5,055,663 + Pf6N2E2_4333 0.41 -0.4 5,055,664 - Pf6N2E2_4333 0.41 +0.2 5,055,664 - Pf6N2E2_4333 0.41 -1.9 5,055,771 + Pf6N2E2_4333 0.63 +0.3 5,055,771 + Pf6N2E2_4333 0.63 +0.6 5,055,771 + Pf6N2E2_4333 0.63 +0.8 5,055,772 - Pf6N2E2_4333 0.63 +0.2 5,055,772 - Pf6N2E2_4333 0.63 +0.5 5,055,772 - Pf6N2E2_4333 0.63 -0.1 5,055,772 - Pf6N2E2_4333 0.63 +0.8 5,055,772 - Pf6N2E2_4333 0.63 +0.2 5,055,772 - Pf6N2E2_4333 0.63 -0.2 5,055,772 - Pf6N2E2_4333 0.63 +0.5
Or see this region's nucleotide sequence