Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4331

Experiment: D-Glucosamine Hydrochloride (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4329 and Pf6N2E2_4330 overlap by 4 nucleotidesPf6N2E2_4330 and Pf6N2E2_4331 are separated by 47 nucleotidesPf6N2E2_4331 and Pf6N2E2_4332 overlap by 4 nucleotidesPf6N2E2_4332 and Pf6N2E2_4333 are separated by 81 nucleotides Pf6N2E2_4329 - Protein ImpG/VasA, at 5,052,119 to 5,053,978 _4329 Pf6N2E2_4330 - Uncharacterized protein ImpF, at 5,053,975 to 5,054,433 _4330 Pf6N2E2_4331 - hypothetical protein, at 5,054,481 to 5,054,930 _4331 Pf6N2E2_4332 - hypothetical protein, at 5,054,927 to 5,055,382 _4332 Pf6N2E2_4333 - Uncharacterized protein ImpD, at 5,055,464 to 5,055,952 _4333 Position (kb) 5054 5055Strain fitness (log2 ratio) -2 -1 0 1at 5053.536 kb on + strand, within Pf6N2E2_4329at 5053.731 kb on + strand, within Pf6N2E2_4329at 5053.732 kb on - strand, within Pf6N2E2_4329at 5053.846 kb on - strandat 5053.953 kb on + strandat 5053.953 kb on + strandat 5053.953 kb on + strandat 5053.954 kb on - strandat 5053.954 kb on - strandat 5054.503 kb on + strandat 5054.586 kb on + strand, within Pf6N2E2_4331at 5054.587 kb on - strand, within Pf6N2E2_4331at 5054.657 kb on + strand, within Pf6N2E2_4331at 5054.657 kb on + strand, within Pf6N2E2_4331at 5054.657 kb on + strand, within Pf6N2E2_4331at 5054.658 kb on - strand, within Pf6N2E2_4331at 5054.907 kb on + strandat 5054.907 kb on + strandat 5054.908 kb on - strandat 5054.908 kb on - strandat 5054.908 kb on - strandat 5054.930 kb on + strandat 5054.930 kb on + strandat 5054.930 kb on + strandat 5054.931 kb on - strandat 5054.931 kb on - strandat 5054.931 kb on - strandat 5055.219 kb on + strand, within Pf6N2E2_4332at 5055.219 kb on + strand, within Pf6N2E2_4332at 5055.220 kb on - strand, within Pf6N2E2_4332at 5055.220 kb on - strand, within Pf6N2E2_4332at 5055.253 kb on + strand, within Pf6N2E2_4332at 5055.253 kb on + strand, within Pf6N2E2_4332at 5055.254 kb on - strand, within Pf6N2E2_4332at 5055.325 kb on - strand, within Pf6N2E2_4332at 5055.345 kb on + strandat 5055.345 kb on + strandat 5055.345 kb on + strandat 5055.345 kb on + strandat 5055.346 kb on - strandat 5055.391 kb on + strandat 5055.391 kb on + strandat 5055.391 kb on + strandat 5055.392 kb on - strandat 5055.392 kb on - strandat 5055.465 kb on + strandat 5055.465 kb on + strandat 5055.466 kb on - strandat 5055.631 kb on + strand, within Pf6N2E2_4333at 5055.631 kb on + strand, within Pf6N2E2_4333at 5055.632 kb on - strand, within Pf6N2E2_4333at 5055.663 kb on + strand, within Pf6N2E2_4333at 5055.663 kb on + strand, within Pf6N2E2_4333at 5055.663 kb on + strand, within Pf6N2E2_4333at 5055.664 kb on - strand, within Pf6N2E2_4333at 5055.664 kb on - strand, within Pf6N2E2_4333at 5055.771 kb on + strand, within Pf6N2E2_4333at 5055.771 kb on + strand, within Pf6N2E2_4333at 5055.771 kb on + strand, within Pf6N2E2_4333at 5055.772 kb on - strand, within Pf6N2E2_4333at 5055.772 kb on - strand, within Pf6N2E2_4333at 5055.772 kb on - strand, within Pf6N2E2_4333at 5055.772 kb on - strand, within Pf6N2E2_4333at 5055.772 kb on - strand, within Pf6N2E2_4333at 5055.772 kb on - strand, within Pf6N2E2_4333at 5055.772 kb on - strand, within Pf6N2E2_4333

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C)
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5,053,536 + Pf6N2E2_4329 0.76 +0.6
5,053,731 + Pf6N2E2_4329 0.87 -1.2
5,053,732 - Pf6N2E2_4329 0.87 +1.3
5,053,846 - +0.6
5,053,953 + +0.6
5,053,953 + +1.0
5,053,953 + -0.1
5,053,954 - +0.7
5,053,954 - +0.8
5,054,503 + +0.8
5,054,586 + Pf6N2E2_4331 0.23 -0.8
5,054,587 - Pf6N2E2_4331 0.24 -1.8
5,054,657 + Pf6N2E2_4331 0.39 +0.3
5,054,657 + Pf6N2E2_4331 0.39 -0.4
5,054,657 + Pf6N2E2_4331 0.39 -1.6
5,054,658 - Pf6N2E2_4331 0.39 -0.2
5,054,907 + +0.3
5,054,907 + +0.5
5,054,908 - -0.5
5,054,908 - -1.4
5,054,908 - -2.4
5,054,930 + -0.2
5,054,930 + +0.5
5,054,930 + +1.1
5,054,931 - +1.2
5,054,931 - +0.4
5,054,931 - +0.9
5,055,219 + Pf6N2E2_4332 0.64 +0.9
5,055,219 + Pf6N2E2_4332 0.64 +0.6
5,055,220 - Pf6N2E2_4332 0.64 +0.1
5,055,220 - Pf6N2E2_4332 0.64 -0.3
5,055,253 + Pf6N2E2_4332 0.71 +0.5
5,055,253 + Pf6N2E2_4332 0.71 +1.2
5,055,254 - Pf6N2E2_4332 0.72 -0.2
5,055,325 - Pf6N2E2_4332 0.87 +0.8
5,055,345 + +0.7
5,055,345 + +0.3
5,055,345 + +0.4
5,055,345 + -0.8
5,055,346 - -0.4
5,055,391 + +0.9
5,055,391 + -1.1
5,055,391 + +0.4
5,055,392 - -0.6
5,055,392 - +0.8
5,055,465 + +1.1
5,055,465 + +1.7
5,055,466 - -0.3
5,055,631 + Pf6N2E2_4333 0.34 +0.6
5,055,631 + Pf6N2E2_4333 0.34 +0.4
5,055,632 - Pf6N2E2_4333 0.34 +0.1
5,055,663 + Pf6N2E2_4333 0.41 +0.4
5,055,663 + Pf6N2E2_4333 0.41 -0.2
5,055,663 + Pf6N2E2_4333 0.41 -0.4
5,055,664 - Pf6N2E2_4333 0.41 +0.2
5,055,664 - Pf6N2E2_4333 0.41 -1.9
5,055,771 + Pf6N2E2_4333 0.63 +0.3
5,055,771 + Pf6N2E2_4333 0.63 +0.6
5,055,771 + Pf6N2E2_4333 0.63 +0.8
5,055,772 - Pf6N2E2_4333 0.63 +0.2
5,055,772 - Pf6N2E2_4333 0.63 +0.5
5,055,772 - Pf6N2E2_4333 0.63 -0.1
5,055,772 - Pf6N2E2_4333 0.63 +0.8
5,055,772 - Pf6N2E2_4333 0.63 +0.2
5,055,772 - Pf6N2E2_4333 0.63 -0.2
5,055,772 - Pf6N2E2_4333 0.63 +0.5

Or see this region's nucleotide sequence