Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3337 and Pf6N2E2_3338 are separated by 28 nucleotides Pf6N2E2_3338 and Pf6N2E2_3339 overlap by 1 nucleotides
Pf6N2E2_3337 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 3,860,717 to 3,862,444
_3337
Pf6N2E2_3338 - 1-phosphofructokinase (EC 2.7.1.56), at 3,862,473 to 3,863,414
_3338
Pf6N2E2_3339 - Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9), at 3,863,414 to 3,866,278
_3339
Position (kb)
3862
3863
3864 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3861.546 kb on + strand, within Pf6N2E2_3337 at 3861.546 kb on + strand, within Pf6N2E2_3337 at 3861.555 kb on + strand, within Pf6N2E2_3337 at 3861.555 kb on + strand, within Pf6N2E2_3337 at 3861.556 kb on - strand, within Pf6N2E2_3337 at 3861.738 kb on + strand, within Pf6N2E2_3337 at 3861.738 kb on + strand, within Pf6N2E2_3337 at 3861.739 kb on - strand, within Pf6N2E2_3337 at 3861.868 kb on - strand, within Pf6N2E2_3337 at 3862.451 kb on + strand at 3862.452 kb on - strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3863.168 kb on + strand, within Pf6N2E2_3338 at 3863.168 kb on + strand, within Pf6N2E2_3338 at 3863.168 kb on + strand, within Pf6N2E2_3338 at 3863.415 kb on + strand at 3863.429 kb on + strand at 3863.430 kb on - strand at 3863.486 kb on + strand at 3863.486 kb on + strand at 3863.487 kb on - strand at 3863.745 kb on + strand, within Pf6N2E2_3339 at 3863.746 kb on - strand, within Pf6N2E2_3339 at 3863.811 kb on - strand, within Pf6N2E2_3339 at 3863.811 kb on - strand, within Pf6N2E2_3339 at 3864.072 kb on + strand, within Pf6N2E2_3339
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 3,861,546 + Pf6N2E2_3337 0.48 -1.1 3,861,546 + Pf6N2E2_3337 0.48 -1.3 3,861,555 + Pf6N2E2_3337 0.48 -0.1 3,861,555 + Pf6N2E2_3337 0.48 -1.6 3,861,556 - Pf6N2E2_3337 0.49 -1.7 3,861,738 + Pf6N2E2_3337 0.59 -3.0 3,861,738 + Pf6N2E2_3337 0.59 -1.5 3,861,739 - Pf6N2E2_3337 0.59 -0.1 3,861,868 - Pf6N2E2_3337 0.67 -0.4 3,862,451 + +0.2 3,862,452 - -1.2 3,862,474 + +0.6 3,862,474 + +0.9 3,862,474 + -1.5 3,862,474 + -1.3 3,862,474 + +0.1 3,862,474 + -0.8 3,862,474 + -1.4 3,862,474 + +2.0 3,862,475 - -0.2 3,862,475 - -1.4 3,862,475 - -0.5 3,862,475 - -0.2 3,862,475 - +0.1 3,862,475 - -0.4 3,862,475 - -2.4 3,862,475 - -0.2 3,862,475 - -0.1 3,863,168 + Pf6N2E2_3338 0.74 -1.5 3,863,168 + Pf6N2E2_3338 0.74 +0.9 3,863,168 + Pf6N2E2_3338 0.74 -1.6 3,863,415 + +1.5 3,863,429 + -1.1 3,863,430 - +1.5 3,863,486 + -1.2 3,863,486 + -1.6 3,863,487 - +0.4 3,863,745 + Pf6N2E2_3339 0.12 -0.5 3,863,746 - Pf6N2E2_3339 0.12 +0.5 3,863,811 - Pf6N2E2_3339 0.14 -1.5 3,863,811 - Pf6N2E2_3339 0.14 +0.4 3,864,072 + Pf6N2E2_3339 0.23 +1.1
Or see this region's nucleotide sequence