Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3336 and Pf6N2E2_3337 are separated by 314 nucleotides Pf6N2E2_3337 and Pf6N2E2_3338 are separated by 28 nucleotides Pf6N2E2_3338 and Pf6N2E2_3339 overlap by 1 nucleotides
Pf6N2E2_3336 - Phosphodiesterase/alkaline phosphatase D, at 3,858,861 to 3,860,402
_3336
Pf6N2E2_3337 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 3,860,717 to 3,862,444
_3337
Pf6N2E2_3338 - 1-phosphofructokinase (EC 2.7.1.56), at 3,862,473 to 3,863,414
_3338
Pf6N2E2_3339 - Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9), at 3,863,414 to 3,866,278
_3339
Position (kb)
3860
3861
3862
3863 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3859.735 kb on + strand, within Pf6N2E2_3336 at 3859.736 kb on - strand, within Pf6N2E2_3336 at 3859.742 kb on - strand, within Pf6N2E2_3336 at 3859.858 kb on + strand, within Pf6N2E2_3336 at 3859.858 kb on + strand, within Pf6N2E2_3336 at 3859.858 kb on + strand, within Pf6N2E2_3336 at 3859.859 kb on - strand, within Pf6N2E2_3336 at 3859.859 kb on - strand, within Pf6N2E2_3336 at 3859.859 kb on - strand, within Pf6N2E2_3336 at 3859.915 kb on + strand, within Pf6N2E2_3336 at 3859.915 kb on + strand, within Pf6N2E2_3336 at 3859.916 kb on - strand, within Pf6N2E2_3336 at 3859.916 kb on - strand, within Pf6N2E2_3336 at 3859.916 kb on - strand, within Pf6N2E2_3336 at 3859.916 kb on - strand, within Pf6N2E2_3336 at 3860.246 kb on + strand, within Pf6N2E2_3336 at 3860.246 kb on + strand, within Pf6N2E2_3336 at 3860.246 kb on + strand, within Pf6N2E2_3336 at 3860.246 kb on + strand, within Pf6N2E2_3336 at 3860.430 kb on + strand at 3860.430 kb on + strand at 3860.431 kb on - strand at 3860.431 kb on - strand at 3860.431 kb on - strand at 3860.489 kb on + strand at 3860.490 kb on - strand at 3860.490 kb on - strand at 3860.490 kb on - strand at 3860.490 kb on - strand at 3860.610 kb on + strand at 3860.683 kb on + strand at 3860.683 kb on + strand at 3860.684 kb on - strand at 3860.684 kb on - strand at 3860.747 kb on + strand at 3860.820 kb on + strand at 3860.820 kb on + strand at 3860.821 kb on - strand at 3860.821 kb on - strand at 3860.821 kb on - strand at 3860.821 kb on - strand at 3860.821 kb on - strand at 3860.901 kb on + strand, within Pf6N2E2_3337 at 3861.321 kb on + strand, within Pf6N2E2_3337 at 3861.321 kb on + strand, within Pf6N2E2_3337 at 3861.321 kb on + strand, within Pf6N2E2_3337 at 3861.321 kb on + strand, within Pf6N2E2_3337 at 3861.321 kb on + strand, within Pf6N2E2_3337 at 3861.322 kb on - strand, within Pf6N2E2_3337 at 3861.322 kb on - strand, within Pf6N2E2_3337 at 3861.322 kb on - strand, within Pf6N2E2_3337 at 3861.322 kb on - strand, within Pf6N2E2_3337 at 3861.322 kb on - strand, within Pf6N2E2_3337 at 3861.546 kb on + strand, within Pf6N2E2_3337 at 3861.546 kb on + strand, within Pf6N2E2_3337 at 3861.555 kb on + strand, within Pf6N2E2_3337 at 3861.555 kb on + strand, within Pf6N2E2_3337 at 3861.556 kb on - strand, within Pf6N2E2_3337 at 3861.738 kb on + strand, within Pf6N2E2_3337 at 3861.738 kb on + strand, within Pf6N2E2_3337 at 3861.739 kb on - strand, within Pf6N2E2_3337 at 3861.868 kb on - strand, within Pf6N2E2_3337 at 3862.451 kb on + strand at 3862.452 kb on - strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3863.168 kb on + strand, within Pf6N2E2_3338 at 3863.168 kb on + strand, within Pf6N2E2_3338 at 3863.168 kb on + strand, within Pf6N2E2_3338 at 3863.415 kb on + strand at 3863.429 kb on + strand at 3863.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 3,859,735 + Pf6N2E2_3336 0.57 +2.1 3,859,736 - Pf6N2E2_3336 0.57 +0.5 3,859,742 - Pf6N2E2_3336 0.57 +1.3 3,859,858 + Pf6N2E2_3336 0.65 +0.3 3,859,858 + Pf6N2E2_3336 0.65 -1.0 3,859,858 + Pf6N2E2_3336 0.65 -0.1 3,859,859 - Pf6N2E2_3336 0.65 +0.2 3,859,859 - Pf6N2E2_3336 0.65 -0.2 3,859,859 - Pf6N2E2_3336 0.65 -0.6 3,859,915 + Pf6N2E2_3336 0.68 +0.4 3,859,915 + Pf6N2E2_3336 0.68 +0.1 3,859,916 - Pf6N2E2_3336 0.68 +0.7 3,859,916 - Pf6N2E2_3336 0.68 +0.3 3,859,916 - Pf6N2E2_3336 0.68 -1.1 3,859,916 - Pf6N2E2_3336 0.68 +1.1 3,860,246 + Pf6N2E2_3336 0.90 +0.5 3,860,246 + Pf6N2E2_3336 0.90 -0.3 3,860,246 + Pf6N2E2_3336 0.90 +0.3 3,860,246 + Pf6N2E2_3336 0.90 -0.1 3,860,430 + -0.3 3,860,430 + +0.2 3,860,431 - +0.4 3,860,431 - -0.4 3,860,431 - -0.2 3,860,489 + -1.1 3,860,490 - +0.5 3,860,490 - -0.1 3,860,490 - -0.1 3,860,490 - -0.5 3,860,610 + +0.4 3,860,683 + +1.1 3,860,683 + -1.0 3,860,684 - -0.2 3,860,684 - +0.2 3,860,747 + -0.4 3,860,820 + -1.5 3,860,820 + -2.7 3,860,821 - +1.5 3,860,821 - -1.7 3,860,821 - -0.0 3,860,821 - -1.2 3,860,821 - +0.1 3,860,901 + Pf6N2E2_3337 0.11 -0.4 3,861,321 + Pf6N2E2_3337 0.35 -1.6 3,861,321 + Pf6N2E2_3337 0.35 -1.1 3,861,321 + Pf6N2E2_3337 0.35 +0.1 3,861,321 + Pf6N2E2_3337 0.35 -1.9 3,861,321 + Pf6N2E2_3337 0.35 -0.3 3,861,322 - Pf6N2E2_3337 0.35 -2.3 3,861,322 - Pf6N2E2_3337 0.35 -2.1 3,861,322 - Pf6N2E2_3337 0.35 -0.9 3,861,322 - Pf6N2E2_3337 0.35 -2.1 3,861,322 - Pf6N2E2_3337 0.35 -0.7 3,861,546 + Pf6N2E2_3337 0.48 -1.1 3,861,546 + Pf6N2E2_3337 0.48 -1.3 3,861,555 + Pf6N2E2_3337 0.48 -0.1 3,861,555 + Pf6N2E2_3337 0.48 -1.6 3,861,556 - Pf6N2E2_3337 0.49 -1.7 3,861,738 + Pf6N2E2_3337 0.59 -3.0 3,861,738 + Pf6N2E2_3337 0.59 -1.5 3,861,739 - Pf6N2E2_3337 0.59 -0.1 3,861,868 - Pf6N2E2_3337 0.67 -0.4 3,862,451 + +0.2 3,862,452 - -1.2 3,862,474 + +0.6 3,862,474 + +0.9 3,862,474 + -1.5 3,862,474 + -1.3 3,862,474 + +0.1 3,862,474 + -0.8 3,862,474 + -1.4 3,862,474 + +2.0 3,862,475 - -0.2 3,862,475 - -1.4 3,862,475 - -0.5 3,862,475 - -0.2 3,862,475 - +0.1 3,862,475 - -0.4 3,862,475 - -2.4 3,862,475 - -0.2 3,862,475 - -0.1 3,863,168 + Pf6N2E2_3338 0.74 -1.5 3,863,168 + Pf6N2E2_3338 0.74 +0.9 3,863,168 + Pf6N2E2_3338 0.74 -1.6 3,863,415 + +1.5 3,863,429 + -1.1 3,863,430 - +1.5
Or see this region's nucleotide sequence