Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3252 and Pf6N2E2_3253 are separated by 123 nucleotides Pf6N2E2_3253 and Pf6N2E2_3254 are separated by 127 nucleotides
Pf6N2E2_3252 - Histidinol dehydrogenase (EC 1.1.1.23), at 3,762,472 to 3,763,809
_3252
Pf6N2E2_3253 - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 3,763,933 to 3,764,568
_3253
Pf6N2E2_3254 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 3,764,696 to 3,765,961
_3254
Position (kb)
3763
3764
3765 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3763.145 kb on + strand, within Pf6N2E2_3252 at 3763.145 kb on + strand, within Pf6N2E2_3252 at 3763.145 kb on + strand, within Pf6N2E2_3252 at 3763.145 kb on + strand, within Pf6N2E2_3252 at 3763.145 kb on + strand, within Pf6N2E2_3252 at 3763.145 kb on + strand, within Pf6N2E2_3252 at 3763.145 kb on + strand, within Pf6N2E2_3252 at 3763.145 kb on + strand, within Pf6N2E2_3252 at 3763.145 kb on + strand, within Pf6N2E2_3252 at 3763.145 kb on + strand, within Pf6N2E2_3252 at 3763.146 kb on - strand, within Pf6N2E2_3252 at 3763.146 kb on - strand, within Pf6N2E2_3252 at 3763.146 kb on - strand, within Pf6N2E2_3252 at 3763.146 kb on - strand, within Pf6N2E2_3252 at 3763.146 kb on - strand, within Pf6N2E2_3252 at 3763.379 kb on + strand, within Pf6N2E2_3252 at 3763.404 kb on - strand, within Pf6N2E2_3252 at 3763.404 kb on - strand, within Pf6N2E2_3252 at 3763.852 kb on + strand at 3763.853 kb on - strand at 3763.853 kb on - strand at 3763.889 kb on + strand at 3764.159 kb on + strand, within Pf6N2E2_3253 at 3764.159 kb on + strand, within Pf6N2E2_3253 at 3764.159 kb on + strand, within Pf6N2E2_3253 at 3764.160 kb on - strand, within Pf6N2E2_3253 at 3764.160 kb on - strand, within Pf6N2E2_3253 at 3764.160 kb on - strand, within Pf6N2E2_3253 at 3764.199 kb on - strand, within Pf6N2E2_3253 at 3764.201 kb on + strand, within Pf6N2E2_3253 at 3764.202 kb on - strand, within Pf6N2E2_3253 at 3764.202 kb on - strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.585 kb on + strand at 3764.593 kb on + strand at 3764.594 kb on - strand at 3764.594 kb on - strand at 3764.594 kb on - strand at 3764.594 kb on - strand at 3764.697 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 3,763,145 + Pf6N2E2_3252 0.50 -1.9 3,763,145 + Pf6N2E2_3252 0.50 -1.7 3,763,145 + Pf6N2E2_3252 0.50 +0.2 3,763,145 + Pf6N2E2_3252 0.50 -1.9 3,763,145 + Pf6N2E2_3252 0.50 +0.1 3,763,145 + Pf6N2E2_3252 0.50 -2.7 3,763,145 + Pf6N2E2_3252 0.50 -0.7 3,763,145 + Pf6N2E2_3252 0.50 -2.2 3,763,145 + Pf6N2E2_3252 0.50 -2.4 3,763,145 + Pf6N2E2_3252 0.50 -1.4 3,763,146 - Pf6N2E2_3252 0.50 -2.1 3,763,146 - Pf6N2E2_3252 0.50 -2.8 3,763,146 - Pf6N2E2_3252 0.50 -1.1 3,763,146 - Pf6N2E2_3252 0.50 -2.9 3,763,146 - Pf6N2E2_3252 0.50 +0.8 3,763,379 + Pf6N2E2_3252 0.68 -2.4 3,763,404 - Pf6N2E2_3252 0.70 -1.6 3,763,404 - Pf6N2E2_3252 0.70 +0.1 3,763,852 + -2.1 3,763,853 - +1.2 3,763,853 - +0.6 3,763,889 + -1.2 3,764,159 + Pf6N2E2_3253 0.36 -1.2 3,764,159 + Pf6N2E2_3253 0.36 -3.0 3,764,159 + Pf6N2E2_3253 0.36 -2.5 3,764,160 - Pf6N2E2_3253 0.36 -0.7 3,764,160 - Pf6N2E2_3253 0.36 -3.1 3,764,160 - Pf6N2E2_3253 0.36 -1.9 3,764,199 - Pf6N2E2_3253 0.42 -0.8 3,764,201 + Pf6N2E2_3253 0.42 +0.6 3,764,202 - Pf6N2E2_3253 0.42 -3.1 3,764,202 - Pf6N2E2_3253 0.42 -1.8 3,764,396 + Pf6N2E2_3253 0.73 -2.0 3,764,396 + Pf6N2E2_3253 0.73 -1.1 3,764,396 + Pf6N2E2_3253 0.73 -2.6 3,764,396 + Pf6N2E2_3253 0.73 +0.8 3,764,585 + -2.1 3,764,593 + -2.4 3,764,594 - -0.0 3,764,594 - +0.7 3,764,594 - +1.3 3,764,594 - -0.2 3,764,697 + -0.6
Or see this region's nucleotide sequence