Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2572 and Pf6N2E2_2573 are separated by 155 nucleotides Pf6N2E2_2573 and Pf6N2E2_2574 overlap by 4 nucleotides Pf6N2E2_2574 and Pf6N2E2_2575 are separated by 116 nucleotides
Pf6N2E2_2572 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 3,030,664 to 3,031,047
_2572
Pf6N2E2_2573 - RNA polymerase sigma factor for flagellar operon, at 3,031,203 to 3,031,946
_2573
Pf6N2E2_2574 - Flagellar synthesis regulator FleN, at 3,031,943 to 3,032,773
_2574
Pf6N2E2_2575 - Flagellar biosynthesis protein FlhF, at 3,032,890 to 3,034,239
_2575
Position (kb)
3031
3032
3033 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3030.988 kb on + strand, within Pf6N2E2_2572 at 3031.067 kb on + strand at 3031.067 kb on + strand at 3031.068 kb on - strand at 3031.154 kb on + strand at 3031.155 kb on - strand at 3031.155 kb on - strand at 3031.261 kb on - strand at 3031.261 kb on - strand at 3031.334 kb on - strand, within Pf6N2E2_2573 at 3031.334 kb on - strand, within Pf6N2E2_2573 at 3031.334 kb on - strand, within Pf6N2E2_2573 at 3031.520 kb on + strand, within Pf6N2E2_2573 at 3031.520 kb on + strand, within Pf6N2E2_2573 at 3031.521 kb on - strand, within Pf6N2E2_2573 at 3031.540 kb on + strand, within Pf6N2E2_2573 at 3031.586 kb on + strand, within Pf6N2E2_2573 at 3031.604 kb on + strand, within Pf6N2E2_2573 at 3031.644 kb on - strand, within Pf6N2E2_2573 at 3031.723 kb on + strand, within Pf6N2E2_2573 at 3031.723 kb on + strand, within Pf6N2E2_2573 at 3031.724 kb on - strand, within Pf6N2E2_2573 at 3031.724 kb on - strand, within Pf6N2E2_2573 at 3031.724 kb on - strand, within Pf6N2E2_2573 at 3031.757 kb on - strand, within Pf6N2E2_2573 at 3031.846 kb on + strand, within Pf6N2E2_2573 at 3031.846 kb on + strand, within Pf6N2E2_2573 at 3031.847 kb on - strand, within Pf6N2E2_2573 at 3031.892 kb on - strand at 3031.919 kb on - strand at 3031.919 kb on - strand at 3031.931 kb on - strand at 3031.931 kb on - strand at 3031.947 kb on - strand at 3032.154 kb on + strand, within Pf6N2E2_2574 at 3032.154 kb on + strand, within Pf6N2E2_2574 at 3032.271 kb on + strand, within Pf6N2E2_2574 at 3032.795 kb on - strand at 3032.795 kb on - strand at 3032.798 kb on + strand at 3032.799 kb on - strand at 3032.833 kb on + strand at 3032.834 kb on - strand at 3032.834 kb on - strand at 3032.834 kb on - strand at 3032.863 kb on + strand at 3032.863 kb on + strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.868 kb on - strand at 3032.887 kb on - strand at 3032.887 kb on - strand at 3032.887 kb on - strand at 3032.887 kb on - strand at 3032.918 kb on + strand at 3032.919 kb on - strand at 3032.919 kb on - strand at 3032.919 kb on - strand at 3032.919 kb on - strand at 3032.923 kb on - strand at 3032.923 kb on - strand at 3032.923 kb on - strand at 3032.935 kb on - strand at 3032.935 kb on - strand at 3032.935 kb on - strand at 3032.935 kb on - strand at 3033.065 kb on - strand, within Pf6N2E2_2575 at 3033.065 kb on - strand, within Pf6N2E2_2575 at 3033.065 kb on - strand, within Pf6N2E2_2575 at 3033.159 kb on - strand, within Pf6N2E2_2575 at 3033.159 kb on - strand, within Pf6N2E2_2575 at 3033.159 kb on - strand, within Pf6N2E2_2575 at 3033.159 kb on - strand, within Pf6N2E2_2575 at 3033.168 kb on - strand, within Pf6N2E2_2575 at 3033.168 kb on - strand, within Pf6N2E2_2575 at 3033.308 kb on - strand, within Pf6N2E2_2575 at 3033.308 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 3,030,988 + Pf6N2E2_2572 0.84 -1.7 3,031,067 + +0.5 3,031,067 + +1.4 3,031,068 - -0.2 3,031,154 + +1.4 3,031,155 - +0.2 3,031,155 - +0.1 3,031,261 - +0.6 3,031,261 - +0.3 3,031,334 - Pf6N2E2_2573 0.18 +0.2 3,031,334 - Pf6N2E2_2573 0.18 -1.5 3,031,334 - Pf6N2E2_2573 0.18 +0.7 3,031,520 + Pf6N2E2_2573 0.43 -0.9 3,031,520 + Pf6N2E2_2573 0.43 +2.3 3,031,521 - Pf6N2E2_2573 0.43 +1.3 3,031,540 + Pf6N2E2_2573 0.45 +0.0 3,031,586 + Pf6N2E2_2573 0.51 -0.5 3,031,604 + Pf6N2E2_2573 0.54 +0.7 3,031,644 - Pf6N2E2_2573 0.59 -0.4 3,031,723 + Pf6N2E2_2573 0.70 +0.7 3,031,723 + Pf6N2E2_2573 0.70 -0.1 3,031,724 - Pf6N2E2_2573 0.70 -1.3 3,031,724 - Pf6N2E2_2573 0.70 +1.3 3,031,724 - Pf6N2E2_2573 0.70 +0.8 3,031,757 - Pf6N2E2_2573 0.74 +0.6 3,031,846 + Pf6N2E2_2573 0.86 +0.8 3,031,846 + Pf6N2E2_2573 0.86 -1.0 3,031,847 - Pf6N2E2_2573 0.87 +0.9 3,031,892 - +0.5 3,031,919 - +0.6 3,031,919 - +0.7 3,031,931 - +1.7 3,031,931 - +0.4 3,031,947 - -0.2 3,032,154 + Pf6N2E2_2574 0.25 -1.7 3,032,154 + Pf6N2E2_2574 0.25 +1.3 3,032,271 + Pf6N2E2_2574 0.39 -1.5 3,032,795 - -0.4 3,032,795 - +0.3 3,032,798 + -0.7 3,032,799 - +0.3 3,032,833 + -2.7 3,032,834 - +0.1 3,032,834 - -0.3 3,032,834 - +0.4 3,032,863 + -1.0 3,032,863 + -0.7 3,032,864 - -1.2 3,032,864 - -1.3 3,032,864 - +2.3 3,032,864 - -0.6 3,032,864 - -0.9 3,032,864 - -0.4 3,032,864 - -2.1 3,032,864 - -0.6 3,032,868 - -0.2 3,032,887 - +0.1 3,032,887 - -0.5 3,032,887 - -0.4 3,032,887 - +2.4 3,032,918 + -2.0 3,032,919 - +0.1 3,032,919 - +0.1 3,032,919 - +1.3 3,032,919 - -0.1 3,032,923 - -0.2 3,032,923 - +0.2 3,032,923 - +0.0 3,032,935 - -0.3 3,032,935 - -3.2 3,032,935 - +1.1 3,032,935 - +0.3 3,033,065 - Pf6N2E2_2575 0.13 +0.3 3,033,065 - Pf6N2E2_2575 0.13 +0.4 3,033,065 - Pf6N2E2_2575 0.13 +0.1 3,033,159 - Pf6N2E2_2575 0.20 -0.9 3,033,159 - Pf6N2E2_2575 0.20 +1.2 3,033,159 - Pf6N2E2_2575 0.20 +0.7 3,033,159 - Pf6N2E2_2575 0.20 -0.0 3,033,168 - Pf6N2E2_2575 0.21 +0.7 3,033,168 - Pf6N2E2_2575 0.21 -0.1 3,033,308 - Pf6N2E2_2575 0.31 -0.5 3,033,308 - Pf6N2E2_2575 0.31 -0.3 3,033,479 - Pf6N2E2_2575 0.44 -1.5 3,033,479 - Pf6N2E2_2575 0.44 -0.1 3,033,479 - Pf6N2E2_2575 0.44 +1.8 3,033,479 - Pf6N2E2_2575 0.44 -0.9 3,033,479 - Pf6N2E2_2575 0.44 +0.7 3,033,479 - Pf6N2E2_2575 0.44 -1.7 3,033,479 - Pf6N2E2_2575 0.44 +0.7 3,033,479 - Pf6N2E2_2575 0.44 -0.5
Or see this region's nucleotide sequence