Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2467 and Pf6N2E2_2468 are separated by 20 nucleotides Pf6N2E2_2468 and Pf6N2E2_2469 are separated by 51 nucleotides Pf6N2E2_2469 and Pf6N2E2_2470 are separated by 16 nucleotides
Pf6N2E2_2467 - 5'-nucleotidase (EC 3.1.3.5), at 2,916,158 to 2,917,063
_2467
Pf6N2E2_2468 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 2,917,084 to 2,918,037
_2468
Pf6N2E2_2469 - hypothetical protein, at 2,918,089 to 2,918,289
_2469
Pf6N2E2_2470 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 2,918,306 to 2,919,382
_2470
Position (kb)
2917
2918
2919 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2916.104 kb on + strand at 2916.104 kb on + strand at 2916.104 kb on + strand at 2916.104 kb on + strand at 2916.104 kb on + strand at 2916.105 kb on - strand at 2916.105 kb on - strand at 2916.105 kb on - strand at 2916.328 kb on + strand, within Pf6N2E2_2467 at 2916.329 kb on - strand, within Pf6N2E2_2467 at 2916.398 kb on - strand, within Pf6N2E2_2467 at 2916.541 kb on - strand, within Pf6N2E2_2467 at 2916.726 kb on + strand, within Pf6N2E2_2467 at 2916.981 kb on + strand at 2916.981 kb on + strand at 2916.981 kb on + strand at 2916.981 kb on + strand at 2916.982 kb on - strand at 2916.982 kb on - strand at 2917.092 kb on + strand at 2917.092 kb on + strand at 2917.093 kb on - strand at 2917.093 kb on - strand at 2917.095 kb on - strand at 2917.095 kb on - strand at 2917.095 kb on - strand at 2917.121 kb on + strand at 2917.122 kb on - strand at 2917.122 kb on - strand at 2917.122 kb on - strand at 2917.122 kb on - strand at 2917.184 kb on + strand, within Pf6N2E2_2468 at 2917.185 kb on - strand, within Pf6N2E2_2468 at 2917.220 kb on + strand, within Pf6N2E2_2468 at 2917.221 kb on - strand, within Pf6N2E2_2468 at 2917.221 kb on - strand at 2917.229 kb on + strand, within Pf6N2E2_2468 at 2917.229 kb on + strand, within Pf6N2E2_2468 at 2917.229 kb on + strand, within Pf6N2E2_2468 at 2917.229 kb on + strand, within Pf6N2E2_2468 at 2917.230 kb on - strand, within Pf6N2E2_2468 at 2917.245 kb on - strand, within Pf6N2E2_2468 at 2917.277 kb on + strand, within Pf6N2E2_2468 at 2917.278 kb on - strand, within Pf6N2E2_2468 at 2917.300 kb on - strand, within Pf6N2E2_2468 at 2917.365 kb on + strand, within Pf6N2E2_2468 at 2917.392 kb on + strand, within Pf6N2E2_2468 at 2917.425 kb on - strand, within Pf6N2E2_2468 at 2917.425 kb on - strand, within Pf6N2E2_2468 at 2917.425 kb on - strand, within Pf6N2E2_2468 at 2918.056 kb on - strand at 2918.073 kb on + strand at 2918.073 kb on + strand at 2918.073 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.108 kb on - strand at 2918.108 kb on - strand at 2918.161 kb on + strand, within Pf6N2E2_2469 at 2918.162 kb on - strand, within Pf6N2E2_2469 at 2918.162 kb on - strand, within Pf6N2E2_2469 at 2918.163 kb on + strand, within Pf6N2E2_2469 at 2918.172 kb on - strand, within Pf6N2E2_2469 at 2918.238 kb on - strand, within Pf6N2E2_2469 at 2918.238 kb on - strand, within Pf6N2E2_2469 at 2918.300 kb on - strand at 2918.333 kb on - strand at 2918.523 kb on - strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.571 kb on - strand, within Pf6N2E2_2470 at 2918.571 kb on - strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.589 kb on - strand, within Pf6N2E2_2470 at 2918.642 kb on + strand, within Pf6N2E2_2470 at 2918.642 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.990 kb on + strand, within Pf6N2E2_2470 at 2918.991 kb on - strand, within Pf6N2E2_2470 at 2919.032 kb on + strand, within Pf6N2E2_2470 at 2919.033 kb on - strand, within Pf6N2E2_2470 at 2919.033 kb on - strand, within Pf6N2E2_2470
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 2,916,104 + +0.4 2,916,104 + -0.7 2,916,104 + -0.4 2,916,104 + +0.4 2,916,104 + -0.7 2,916,105 - +2.3 2,916,105 - +2.9 2,916,105 - +3.2 2,916,328 + Pf6N2E2_2467 0.19 -0.5 2,916,329 - Pf6N2E2_2467 0.19 +1.7 2,916,398 - Pf6N2E2_2467 0.26 +1.8 2,916,541 - Pf6N2E2_2467 0.42 +0.5 2,916,726 + Pf6N2E2_2467 0.63 +0.7 2,916,981 + +0.7 2,916,981 + -0.6 2,916,981 + -0.8 2,916,981 + +0.4 2,916,982 - -1.4 2,916,982 - -0.5 2,917,092 + +1.2 2,917,092 + -0.8 2,917,093 - +1.5 2,917,093 - +1.5 2,917,095 - +1.9 2,917,095 - +1.8 2,917,095 - +2.3 2,917,121 + -0.6 2,917,122 - +2.8 2,917,122 - +2.4 2,917,122 - +1.8 2,917,122 - +2.2 2,917,184 + Pf6N2E2_2468 0.10 -0.2 2,917,185 - Pf6N2E2_2468 0.11 +1.6 2,917,220 + Pf6N2E2_2468 0.14 +0.7 2,917,221 - Pf6N2E2_2468 0.14 +2.1 2,917,221 - +0.8 2,917,229 + Pf6N2E2_2468 0.15 -0.1 2,917,229 + Pf6N2E2_2468 0.15 +0.7 2,917,229 + Pf6N2E2_2468 0.15 -1.1 2,917,229 + Pf6N2E2_2468 0.15 -0.6 2,917,230 - Pf6N2E2_2468 0.15 +0.8 2,917,245 - Pf6N2E2_2468 0.17 +1.5 2,917,277 + Pf6N2E2_2468 0.20 +0.4 2,917,278 - Pf6N2E2_2468 0.20 +1.6 2,917,300 - Pf6N2E2_2468 0.23 +1.2 2,917,365 + Pf6N2E2_2468 0.29 -0.6 2,917,392 + Pf6N2E2_2468 0.32 +0.6 2,917,425 - Pf6N2E2_2468 0.36 +1.2 2,917,425 - Pf6N2E2_2468 0.36 +0.9 2,917,425 - Pf6N2E2_2468 0.36 +3.1 2,918,056 - +2.0 2,918,073 + +1.3 2,918,073 + +0.3 2,918,073 + -2.8 2,918,107 + +0.3 2,918,107 + +0.5 2,918,107 + +0.4 2,918,107 + +0.2 2,918,107 + +0.1 2,918,107 + +1.3 2,918,107 + +1.0 2,918,107 + +1.4 2,918,108 - +2.1 2,918,108 - +1.0 2,918,161 + Pf6N2E2_2469 0.36 +0.5 2,918,162 - Pf6N2E2_2469 0.36 +2.3 2,918,162 - Pf6N2E2_2469 0.36 +2.6 2,918,163 + Pf6N2E2_2469 0.37 -0.2 2,918,172 - Pf6N2E2_2469 0.41 +1.5 2,918,238 - Pf6N2E2_2469 0.74 +2.5 2,918,238 - Pf6N2E2_2469 0.74 +2.9 2,918,300 - +1.8 2,918,333 - +3.4 2,918,523 - Pf6N2E2_2470 0.20 +2.6 2,918,570 + Pf6N2E2_2470 0.25 -0.4 2,918,570 + Pf6N2E2_2470 0.25 +1.6 2,918,570 + Pf6N2E2_2470 0.25 +0.7 2,918,570 + Pf6N2E2_2470 0.25 -0.2 2,918,571 - Pf6N2E2_2470 0.25 +1.5 2,918,571 - Pf6N2E2_2470 0.25 +0.6 2,918,588 + Pf6N2E2_2470 0.26 +1.6 2,918,588 + Pf6N2E2_2470 0.26 -0.5 2,918,588 + Pf6N2E2_2470 0.26 +0.1 2,918,589 - Pf6N2E2_2470 0.26 +1.2 2,918,642 + Pf6N2E2_2470 0.31 +0.1 2,918,642 + Pf6N2E2_2470 0.31 +0.1 2,918,762 + Pf6N2E2_2470 0.42 +1.1 2,918,762 + Pf6N2E2_2470 0.42 +0.2 2,918,762 + Pf6N2E2_2470 0.42 -0.7 2,918,762 + Pf6N2E2_2470 0.42 +0.8 2,918,763 - Pf6N2E2_2470 0.42 +2.5 2,918,763 - Pf6N2E2_2470 0.42 +2.4 2,918,763 - Pf6N2E2_2470 0.42 +1.5 2,918,763 - Pf6N2E2_2470 0.42 +0.9 2,918,990 + Pf6N2E2_2470 0.64 -0.4 2,918,991 - Pf6N2E2_2470 0.64 +2.5 2,919,032 + Pf6N2E2_2470 0.67 +1.1 2,919,033 - Pf6N2E2_2470 0.68 +1.2 2,919,033 - Pf6N2E2_2470 0.68 +2.0
Or see this region's nucleotide sequence