Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_210 and Pf6N2E2_211 are separated by 92 nucleotides Pf6N2E2_211 and Pf6N2E2_212 overlap by 8 nucleotides Pf6N2E2_212 and Pf6N2E2_213 are separated by 48 nucleotides Pf6N2E2_213 and Pf6N2E2_214 are separated by 25 nucleotides
Pf6N2E2_210 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 264,376 to 267,099
_210
Pf6N2E2_211 - SanC, at 267,192 to 267,779
_211
Pf6N2E2_212 - Glycosyl transferase, group 2 family protein, at 267,772 to 269,946
_212
Pf6N2E2_213 - hypothetical protein, at 269,995 to 270,831
_213
Pf6N2E2_214 - dTDP-Rha:A-D-GlcNAc-diphosphoryl polyprenol, A-3-L-rhamnosyl transferase WbbL, at 270,857 to 271,807
_214
Position (kb)
267
268
269
270 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 267.097 kb on + strand at 267.097 kb on + strand at 267.097 kb on + strand at 267.097 kb on + strand at 267.098 kb on - strand at 267.098 kb on - strand at 267.098 kb on - strand at 267.098 kb on - strand at 267.256 kb on - strand, within Pf6N2E2_211 at 267.256 kb on - strand, within Pf6N2E2_211 at 267.256 kb on - strand, within Pf6N2E2_211 at 267.261 kb on + strand, within Pf6N2E2_211 at 267.261 kb on + strand, within Pf6N2E2_211 at 267.261 kb on + strand, within Pf6N2E2_211 at 267.261 kb on + strand, within Pf6N2E2_211 at 267.261 kb on + strand, within Pf6N2E2_211 at 267.262 kb on - strand, within Pf6N2E2_211 at 267.262 kb on - strand, within Pf6N2E2_211 at 267.262 kb on - strand, within Pf6N2E2_211 at 267.504 kb on + strand, within Pf6N2E2_211 at 267.505 kb on - strand, within Pf6N2E2_211 at 267.623 kb on - strand, within Pf6N2E2_211 at 267.706 kb on - strand, within Pf6N2E2_211 at 267.777 kb on + strand at 267.777 kb on + strand at 267.778 kb on - strand at 267.850 kb on - strand at 268.153 kb on + strand, within Pf6N2E2_212 at 268.153 kb on + strand, within Pf6N2E2_212 at 268.153 kb on + strand, within Pf6N2E2_212 at 268.154 kb on - strand, within Pf6N2E2_212 at 268.154 kb on - strand, within Pf6N2E2_212 at 268.154 kb on - strand, within Pf6N2E2_212 at 268.154 kb on - strand, within Pf6N2E2_212 at 268.193 kb on - strand, within Pf6N2E2_212 at 268.258 kb on + strand, within Pf6N2E2_212 at 268.271 kb on + strand, within Pf6N2E2_212 at 268.362 kb on + strand, within Pf6N2E2_212 at 268.362 kb on + strand, within Pf6N2E2_212 at 268.369 kb on + strand, within Pf6N2E2_212 at 268.423 kb on + strand, within Pf6N2E2_212 at 268.423 kb on + strand, within Pf6N2E2_212 at 268.423 kb on + strand, within Pf6N2E2_212 at 268.423 kb on + strand, within Pf6N2E2_212 at 268.537 kb on + strand, within Pf6N2E2_212 at 268.538 kb on - strand, within Pf6N2E2_212 at 268.666 kb on + strand, within Pf6N2E2_212 at 268.841 kb on - strand, within Pf6N2E2_212 at 268.919 kb on - strand, within Pf6N2E2_212 at 268.925 kb on - strand, within Pf6N2E2_212 at 268.925 kb on - strand, within Pf6N2E2_212 at 268.925 kb on - strand, within Pf6N2E2_212 at 268.994 kb on - strand, within Pf6N2E2_212 at 268.994 kb on - strand, within Pf6N2E2_212 at 269.065 kb on + strand, within Pf6N2E2_212 at 269.066 kb on - strand, within Pf6N2E2_212 at 269.066 kb on - strand, within Pf6N2E2_212 at 269.066 kb on - strand, within Pf6N2E2_212 at 269.066 kb on - strand, within Pf6N2E2_212 at 269.066 kb on - strand, within Pf6N2E2_212 at 269.176 kb on + strand, within Pf6N2E2_212 at 269.176 kb on + strand, within Pf6N2E2_212 at 269.176 kb on + strand, within Pf6N2E2_212 at 269.176 kb on + strand, within Pf6N2E2_212 at 269.177 kb on - strand, within Pf6N2E2_212 at 269.227 kb on - strand, within Pf6N2E2_212 at 269.429 kb on + strand, within Pf6N2E2_212 at 269.430 kb on - strand, within Pf6N2E2_212 at 269.514 kb on - strand at 269.720 kb on - strand, within Pf6N2E2_212 at 269.827 kb on + strand at 269.858 kb on - strand at 270.297 kb on + strand, within Pf6N2E2_213 at 270.297 kb on + strand, within Pf6N2E2_213 at 270.297 kb on + strand, within Pf6N2E2_213 at 270.298 kb on - strand, within Pf6N2E2_213 at 270.298 kb on - strand, within Pf6N2E2_213 at 270.298 kb on - strand, within Pf6N2E2_213 at 270.298 kb on - strand, within Pf6N2E2_213 at 270.298 kb on - strand, within Pf6N2E2_213 at 270.298 kb on - strand, within Pf6N2E2_213 at 270.443 kb on + strand, within Pf6N2E2_213 at 270.443 kb on + strand, within Pf6N2E2_213 at 270.444 kb on - strand, within Pf6N2E2_213 at 270.444 kb on - strand, within Pf6N2E2_213 at 270.444 kb on - strand, within Pf6N2E2_213 at 270.444 kb on - strand, within Pf6N2E2_213 at 270.444 kb on - strand, within Pf6N2E2_213 at 270.638 kb on + strand, within Pf6N2E2_213 at 270.832 kb on + strand at 270.858 kb on + strand at 270.858 kb on + strand at 270.859 kb on - strand at 270.863 kb on + strand at 270.864 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 267,097 + +0.1 267,097 + -0.4 267,097 + +0.9 267,097 + -0.3 267,098 - +0.9 267,098 - +0.9 267,098 - +0.5 267,098 - +0.4 267,256 - Pf6N2E2_211 0.11 +1.5 267,256 - Pf6N2E2_211 0.11 -0.8 267,256 - Pf6N2E2_211 0.11 +0.4 267,261 + Pf6N2E2_211 0.12 -1.3 267,261 + Pf6N2E2_211 0.12 -0.0 267,261 + Pf6N2E2_211 0.12 +0.2 267,261 + Pf6N2E2_211 0.12 -0.5 267,261 + Pf6N2E2_211 0.12 +0.2 267,262 - Pf6N2E2_211 0.12 +1.8 267,262 - Pf6N2E2_211 0.12 +1.6 267,262 - Pf6N2E2_211 0.12 -0.3 267,504 + Pf6N2E2_211 0.53 +1.0 267,505 - Pf6N2E2_211 0.53 +0.7 267,623 - Pf6N2E2_211 0.73 -0.6 267,706 - Pf6N2E2_211 0.87 -0.6 267,777 + -0.7 267,777 + +0.5 267,778 - +0.2 267,850 - -1.0 268,153 + Pf6N2E2_212 0.18 +0.4 268,153 + Pf6N2E2_212 0.18 +0.9 268,153 + Pf6N2E2_212 0.18 +0.5 268,154 - Pf6N2E2_212 0.18 -0.3 268,154 - Pf6N2E2_212 0.18 -0.3 268,154 - Pf6N2E2_212 0.18 +0.9 268,154 - Pf6N2E2_212 0.18 +0.4 268,193 - Pf6N2E2_212 0.19 +1.7 268,258 + Pf6N2E2_212 0.22 +0.3 268,271 + Pf6N2E2_212 0.23 -0.8 268,362 + Pf6N2E2_212 0.27 -0.7 268,362 + Pf6N2E2_212 0.27 -1.3 268,369 + Pf6N2E2_212 0.27 +0.6 268,423 + Pf6N2E2_212 0.30 +0.7 268,423 + Pf6N2E2_212 0.30 +0.9 268,423 + Pf6N2E2_212 0.30 -2.3 268,423 + Pf6N2E2_212 0.30 -0.2 268,537 + Pf6N2E2_212 0.35 +0.1 268,538 - Pf6N2E2_212 0.35 +0.7 268,666 + Pf6N2E2_212 0.41 -0.6 268,841 - Pf6N2E2_212 0.49 -2.3 268,919 - Pf6N2E2_212 0.53 +0.0 268,925 - Pf6N2E2_212 0.53 +0.1 268,925 - Pf6N2E2_212 0.53 +0.7 268,925 - Pf6N2E2_212 0.53 -0.2 268,994 - Pf6N2E2_212 0.56 +0.4 268,994 - Pf6N2E2_212 0.56 +0.3 269,065 + Pf6N2E2_212 0.59 +0.8 269,066 - Pf6N2E2_212 0.59 +0.2 269,066 - Pf6N2E2_212 0.59 +0.3 269,066 - Pf6N2E2_212 0.59 +0.4 269,066 - Pf6N2E2_212 0.59 -0.2 269,066 - Pf6N2E2_212 0.59 +0.3 269,176 + Pf6N2E2_212 0.65 +3.6 269,176 + Pf6N2E2_212 0.65 -0.4 269,176 + Pf6N2E2_212 0.65 -2.3 269,176 + Pf6N2E2_212 0.65 -0.7 269,177 - Pf6N2E2_212 0.65 -0.1 269,227 - Pf6N2E2_212 0.67 -0.7 269,429 + Pf6N2E2_212 0.76 +0.9 269,430 - Pf6N2E2_212 0.76 +1.3 269,514 - +1.9 269,720 - Pf6N2E2_212 0.90 +1.5 269,827 + +0.8 269,858 - +0.3 270,297 + Pf6N2E2_213 0.36 -0.2 270,297 + Pf6N2E2_213 0.36 +1.7 270,297 + Pf6N2E2_213 0.36 +0.0 270,298 - Pf6N2E2_213 0.36 +0.4 270,298 - Pf6N2E2_213 0.36 +0.7 270,298 - Pf6N2E2_213 0.36 -0.5 270,298 - Pf6N2E2_213 0.36 +0.9 270,298 - Pf6N2E2_213 0.36 -0.6 270,298 - Pf6N2E2_213 0.36 -0.2 270,443 + Pf6N2E2_213 0.54 -0.5 270,443 + Pf6N2E2_213 0.54 +0.4 270,444 - Pf6N2E2_213 0.54 +0.4 270,444 - Pf6N2E2_213 0.54 +0.6 270,444 - Pf6N2E2_213 0.54 +0.1 270,444 - Pf6N2E2_213 0.54 -0.3 270,444 - Pf6N2E2_213 0.54 -0.1 270,638 + Pf6N2E2_213 0.77 +0.9 270,832 + -0.8 270,858 + -0.0 270,858 + +0.1 270,859 - -0.3 270,863 + +0.2 270,864 - +0.2
Or see this region's nucleotide sequence