Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2046 and Pf6N2E2_2047 are separated by 138 nucleotides Pf6N2E2_2047 and Pf6N2E2_2048 are separated by 41 nucleotides
Pf6N2E2_2046 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 2,437,860 to 2,438,777
_2046
Pf6N2E2_2047 - D-glucosaminate dehydratase (EC 4.3.1.9) (from data) , at 2,438,916 to 2,440,133
_2047
Pf6N2E2_2048 - Transcriptional regulator, IclR family, at 2,440,175 to 2,440,939
_2048
Position (kb)
2438
2439
2440
2441 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2437.970 kb on - strand, within Pf6N2E2_2046 at 2438.041 kb on + strand, within Pf6N2E2_2046 at 2438.041 kb on + strand, within Pf6N2E2_2046 at 2438.041 kb on + strand, within Pf6N2E2_2046 at 2438.042 kb on - strand, within Pf6N2E2_2046 at 2438.042 kb on - strand, within Pf6N2E2_2046 at 2438.092 kb on - strand, within Pf6N2E2_2046 at 2438.120 kb on - strand, within Pf6N2E2_2046 at 2438.120 kb on - strand, within Pf6N2E2_2046 at 2438.176 kb on - strand, within Pf6N2E2_2046 at 2438.200 kb on + strand, within Pf6N2E2_2046 at 2438.219 kb on - strand, within Pf6N2E2_2046 at 2438.375 kb on - strand, within Pf6N2E2_2046 at 2438.375 kb on - strand, within Pf6N2E2_2046 at 2438.430 kb on - strand, within Pf6N2E2_2046 at 2438.465 kb on - strand, within Pf6N2E2_2046 at 2438.506 kb on + strand, within Pf6N2E2_2046 at 2438.507 kb on - strand, within Pf6N2E2_2046 at 2438.507 kb on - strand, within Pf6N2E2_2046 at 2438.507 kb on - strand, within Pf6N2E2_2046 at 2438.507 kb on - strand, within Pf6N2E2_2046 at 2438.620 kb on + strand, within Pf6N2E2_2046 at 2438.660 kb on - strand, within Pf6N2E2_2046 at 2438.853 kb on + strand at 2438.854 kb on - strand at 2438.918 kb on - strand at 2439.145 kb on + strand, within Pf6N2E2_2047 at 2439.322 kb on + strand, within Pf6N2E2_2047 at 2439.322 kb on + strand, within Pf6N2E2_2047 at 2439.322 kb on + strand, within Pf6N2E2_2047 at 2439.323 kb on - strand, within Pf6N2E2_2047 at 2439.346 kb on + strand, within Pf6N2E2_2047 at 2439.347 kb on - strand, within Pf6N2E2_2047 at 2439.673 kb on + strand, within Pf6N2E2_2047 at 2439.698 kb on + strand, within Pf6N2E2_2047 at 2439.862 kb on + strand, within Pf6N2E2_2047 at 2440.105 kb on - strand at 2440.139 kb on + strand at 2440.139 kb on + strand at 2440.139 kb on + strand at 2440.172 kb on + strand at 2440.173 kb on - strand at 2440.176 kb on + strand at 2440.176 kb on + strand at 2440.176 kb on + strand at 2440.176 kb on + strand at 2440.176 kb on + strand at 2440.176 kb on + strand at 2440.177 kb on - strand at 2440.239 kb on + strand at 2440.240 kb on - strand at 2440.240 kb on - strand at 2440.593 kb on + strand, within Pf6N2E2_2048 at 2440.593 kb on + strand, within Pf6N2E2_2048 at 2440.594 kb on - strand, within Pf6N2E2_2048 at 2440.717 kb on - strand, within Pf6N2E2_2048 at 2440.717 kb on - strand, within Pf6N2E2_2048 at 2440.717 kb on - strand, within Pf6N2E2_2048 at 2440.734 kb on + strand, within Pf6N2E2_2048 at 2440.734 kb on + strand, within Pf6N2E2_2048 at 2440.734 kb on + strand, within Pf6N2E2_2048 at 2440.734 kb on + strand, within Pf6N2E2_2048 at 2440.735 kb on - strand, within Pf6N2E2_2048 at 2440.800 kb on + strand, within Pf6N2E2_2048 at 2440.800 kb on + strand, within Pf6N2E2_2048 at 2440.801 kb on - strand, within Pf6N2E2_2048 at 2440.801 kb on - strand, within Pf6N2E2_2048 at 2440.801 kb on - strand, within Pf6N2E2_2048 at 2440.838 kb on - strand, within Pf6N2E2_2048 at 2440.973 kb on - strand at 2441.047 kb on - strand at 2441.082 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 2,437,970 - Pf6N2E2_2046 0.12 +2.2 2,438,041 + Pf6N2E2_2046 0.20 +0.2 2,438,041 + Pf6N2E2_2046 0.20 -1.5 2,438,041 + Pf6N2E2_2046 0.20 -0.9 2,438,042 - Pf6N2E2_2046 0.20 +0.4 2,438,042 - Pf6N2E2_2046 0.20 -1.0 2,438,092 - Pf6N2E2_2046 0.25 +0.8 2,438,120 - Pf6N2E2_2046 0.28 +0.8 2,438,120 - Pf6N2E2_2046 0.28 +1.1 2,438,176 - Pf6N2E2_2046 0.34 -0.0 2,438,200 + Pf6N2E2_2046 0.37 +0.3 2,438,219 - Pf6N2E2_2046 0.39 +0.7 2,438,375 - Pf6N2E2_2046 0.56 -0.3 2,438,375 - Pf6N2E2_2046 0.56 +0.6 2,438,430 - Pf6N2E2_2046 0.62 +0.2 2,438,465 - Pf6N2E2_2046 0.66 +0.7 2,438,506 + Pf6N2E2_2046 0.70 +0.5 2,438,507 - Pf6N2E2_2046 0.70 +0.8 2,438,507 - Pf6N2E2_2046 0.70 -2.3 2,438,507 - Pf6N2E2_2046 0.70 -0.5 2,438,507 - Pf6N2E2_2046 0.70 +1.0 2,438,620 + Pf6N2E2_2046 0.83 -0.2 2,438,660 - Pf6N2E2_2046 0.87 -0.8 2,438,853 + -0.1 2,438,854 - +0.8 2,438,918 - +1.8 2,439,145 + Pf6N2E2_2047 0.19 -1.9 2,439,322 + Pf6N2E2_2047 0.33 -1.5 2,439,322 + Pf6N2E2_2047 0.33 -0.5 2,439,322 + Pf6N2E2_2047 0.33 -0.7 2,439,323 - Pf6N2E2_2047 0.33 -2.5 2,439,346 + Pf6N2E2_2047 0.35 -2.3 2,439,347 - Pf6N2E2_2047 0.35 -1.5 2,439,673 + Pf6N2E2_2047 0.62 -1.8 2,439,698 + Pf6N2E2_2047 0.64 -3.7 2,439,862 + Pf6N2E2_2047 0.78 -1.3 2,440,105 - -0.2 2,440,139 + -2.3 2,440,139 + -0.9 2,440,139 + -3.0 2,440,172 + -2.1 2,440,173 - +0.3 2,440,176 + -2.9 2,440,176 + -0.2 2,440,176 + -2.5 2,440,176 + -1.7 2,440,176 + -3.1 2,440,176 + -3.5 2,440,177 - -0.2 2,440,239 + -0.2 2,440,240 - -2.8 2,440,240 - -2.5 2,440,593 + Pf6N2E2_2048 0.55 -3.6 2,440,593 + Pf6N2E2_2048 0.55 -3.6 2,440,594 - Pf6N2E2_2048 0.55 -1.6 2,440,717 - Pf6N2E2_2048 0.71 +1.0 2,440,717 - Pf6N2E2_2048 0.71 -0.4 2,440,717 - Pf6N2E2_2048 0.71 +0.3 2,440,734 + Pf6N2E2_2048 0.73 -3.2 2,440,734 + Pf6N2E2_2048 0.73 -3.7 2,440,734 + Pf6N2E2_2048 0.73 -3.0 2,440,734 + Pf6N2E2_2048 0.73 -2.5 2,440,735 - Pf6N2E2_2048 0.73 -0.9 2,440,800 + Pf6N2E2_2048 0.82 -2.6 2,440,800 + Pf6N2E2_2048 0.82 -1.5 2,440,801 - Pf6N2E2_2048 0.82 +1.1 2,440,801 - Pf6N2E2_2048 0.82 -1.7 2,440,801 - Pf6N2E2_2048 0.82 -0.8 2,440,838 - Pf6N2E2_2048 0.87 +0.5 2,440,973 - +1.0 2,441,047 - -2.5 2,441,082 - -0.5
Or see this region's nucleotide sequence