Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1964 and Pf6N2E2_1965 are separated by 54 nucleotides Pf6N2E2_1965 and Pf6N2E2_1966 are separated by 841 nucleotides
Pf6N2E2_1964 - Transcriptional regulator of various polyols utilization, AraC family, at 2,329,338 to 2,330,336
_1964
Pf6N2E2_1965 - Fructokinase (EC 2.7.1.4) (from data) , at 2,330,391 to 2,331,311
_1965
Pf6N2E2_1966 - SAM-dependent methyltransferases, at 2,332,153 to 2,332,833
_1966
Position (kb)
2330
2331
2332 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2329.473 kb on + strand, within Pf6N2E2_1964 at 2329.524 kb on + strand, within Pf6N2E2_1964 at 2329.525 kb on - strand, within Pf6N2E2_1964 at 2329.647 kb on - strand, within Pf6N2E2_1964 at 2329.730 kb on - strand, within Pf6N2E2_1964 at 2329.738 kb on + strand, within Pf6N2E2_1964 at 2329.739 kb on - strand, within Pf6N2E2_1964 at 2329.756 kb on + strand, within Pf6N2E2_1964 at 2329.880 kb on - strand, within Pf6N2E2_1964 at 2329.974 kb on - strand, within Pf6N2E2_1964 at 2329.974 kb on - strand, within Pf6N2E2_1964 at 2329.974 kb on - strand, within Pf6N2E2_1964 at 2329.989 kb on + strand, within Pf6N2E2_1964 at 2329.989 kb on + strand, within Pf6N2E2_1964 at 2330.146 kb on + strand, within Pf6N2E2_1964 at 2330.191 kb on + strand, within Pf6N2E2_1964 at 2330.192 kb on - strand, within Pf6N2E2_1964 at 2330.267 kb on + strand at 2330.268 kb on - strand at 2330.365 kb on - strand at 2330.380 kb on + strand at 2330.381 kb on - strand at 2330.396 kb on + strand at 2330.396 kb on + strand at 2330.397 kb on - strand at 2330.397 kb on - strand at 2330.648 kb on - strand, within Pf6N2E2_1965 at 2330.651 kb on + strand, within Pf6N2E2_1965 at 2330.652 kb on - strand, within Pf6N2E2_1965 at 2330.652 kb on - strand, within Pf6N2E2_1965 at 2330.680 kb on - strand, within Pf6N2E2_1965 at 2330.704 kb on + strand, within Pf6N2E2_1965 at 2330.704 kb on + strand, within Pf6N2E2_1965 at 2330.771 kb on + strand, within Pf6N2E2_1965 at 2330.853 kb on + strand, within Pf6N2E2_1965 at 2330.853 kb on + strand, within Pf6N2E2_1965 at 2330.854 kb on - strand, within Pf6N2E2_1965 at 2330.854 kb on - strand, within Pf6N2E2_1965 at 2330.854 kb on - strand, within Pf6N2E2_1965 at 2330.854 kb on - strand, within Pf6N2E2_1965 at 2330.965 kb on + strand, within Pf6N2E2_1965 at 2330.965 kb on + strand, within Pf6N2E2_1965 at 2330.966 kb on - strand, within Pf6N2E2_1965 at 2330.966 kb on - strand, within Pf6N2E2_1965 at 2330.967 kb on + strand, within Pf6N2E2_1965 at 2330.967 kb on + strand, within Pf6N2E2_1965 at 2331.057 kb on - strand, within Pf6N2E2_1965 at 2331.066 kb on + strand, within Pf6N2E2_1965 at 2331.066 kb on + strand, within Pf6N2E2_1965 at 2331.066 kb on + strand, within Pf6N2E2_1965 at 2331.067 kb on - strand, within Pf6N2E2_1965 at 2331.173 kb on - strand, within Pf6N2E2_1965 at 2331.564 kb on + strand at 2331.570 kb on + strand at 2331.572 kb on + strand at 2331.642 kb on + strand at 2331.643 kb on - strand at 2331.643 kb on - strand at 2331.707 kb on + strand at 2331.726 kb on - strand at 2331.726 kb on - strand at 2331.726 kb on - strand at 2331.786 kb on + strand at 2331.787 kb on - strand at 2331.835 kb on + strand at 2331.836 kb on - strand at 2331.899 kb on - strand at 2331.911 kb on + strand at 2331.911 kb on + strand at 2331.914 kb on - strand at 2331.929 kb on + strand at 2331.929 kb on + strand at 2331.930 kb on - strand at 2332.102 kb on + strand at 2332.103 kb on - strand at 2332.103 kb on - strand at 2332.213 kb on + strand at 2332.217 kb on + strand at 2332.217 kb on + strand at 2332.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 2,329,473 + Pf6N2E2_1964 0.14 -0.2 2,329,524 + Pf6N2E2_1964 0.19 +0.4 2,329,525 - Pf6N2E2_1964 0.19 +0.2 2,329,647 - Pf6N2E2_1964 0.31 +1.5 2,329,730 - Pf6N2E2_1964 0.39 +1.8 2,329,738 + Pf6N2E2_1964 0.40 -0.5 2,329,739 - Pf6N2E2_1964 0.40 +0.7 2,329,756 + Pf6N2E2_1964 0.42 +0.3 2,329,880 - Pf6N2E2_1964 0.54 -0.5 2,329,974 - Pf6N2E2_1964 0.64 -0.2 2,329,974 - Pf6N2E2_1964 0.64 -0.3 2,329,974 - Pf6N2E2_1964 0.64 -0.6 2,329,989 + Pf6N2E2_1964 0.65 -0.7 2,329,989 + Pf6N2E2_1964 0.65 -1.3 2,330,146 + Pf6N2E2_1964 0.81 +1.3 2,330,191 + Pf6N2E2_1964 0.85 -0.5 2,330,192 - Pf6N2E2_1964 0.85 +0.0 2,330,267 + +1.4 2,330,268 - +0.3 2,330,365 - +0.3 2,330,380 + +0.1 2,330,381 - +0.1 2,330,396 + +0.1 2,330,396 + +0.7 2,330,397 - +0.2 2,330,397 - +1.8 2,330,648 - Pf6N2E2_1965 0.28 -0.9 2,330,651 + Pf6N2E2_1965 0.28 -0.3 2,330,652 - Pf6N2E2_1965 0.28 -0.2 2,330,652 - Pf6N2E2_1965 0.28 +1.2 2,330,680 - Pf6N2E2_1965 0.31 +1.3 2,330,704 + Pf6N2E2_1965 0.34 +0.7 2,330,704 + Pf6N2E2_1965 0.34 +0.4 2,330,771 + Pf6N2E2_1965 0.41 +0.5 2,330,853 + Pf6N2E2_1965 0.50 +1.8 2,330,853 + Pf6N2E2_1965 0.50 +0.3 2,330,854 - Pf6N2E2_1965 0.50 +0.3 2,330,854 - Pf6N2E2_1965 0.50 +0.0 2,330,854 - Pf6N2E2_1965 0.50 +1.4 2,330,854 - Pf6N2E2_1965 0.50 -1.3 2,330,965 + Pf6N2E2_1965 0.62 -1.3 2,330,965 + Pf6N2E2_1965 0.62 -0.7 2,330,966 - Pf6N2E2_1965 0.62 +1.8 2,330,966 - Pf6N2E2_1965 0.62 +0.7 2,330,967 + Pf6N2E2_1965 0.63 +1.4 2,330,967 + Pf6N2E2_1965 0.63 -1.3 2,331,057 - Pf6N2E2_1965 0.72 +1.1 2,331,066 + Pf6N2E2_1965 0.73 +1.0 2,331,066 + Pf6N2E2_1965 0.73 -0.3 2,331,066 + Pf6N2E2_1965 0.73 -0.5 2,331,067 - Pf6N2E2_1965 0.73 +0.1 2,331,173 - Pf6N2E2_1965 0.85 +0.0 2,331,564 + +0.5 2,331,570 + +0.7 2,331,572 + -0.7 2,331,642 + +0.6 2,331,643 - +1.4 2,331,643 - +1.5 2,331,707 + +0.6 2,331,726 - +1.1 2,331,726 - +1.4 2,331,726 - +0.5 2,331,786 + +1.1 2,331,787 - -0.6 2,331,835 + -1.2 2,331,836 - +0.3 2,331,899 - +0.3 2,331,911 + -0.4 2,331,911 + -0.9 2,331,914 - -2.1 2,331,929 + -1.2 2,331,929 + -0.3 2,331,930 - +1.4 2,332,102 + -1.2 2,332,103 - +0.4 2,332,103 - +1.1 2,332,213 + +0.0 2,332,217 + +0.7 2,332,217 + -0.2 2,332,218 - +0.8
Or see this region's nucleotide sequence