Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1934 and Pf6N2E2_1935 are separated by 63 nucleotides Pf6N2E2_1935 and Pf6N2E2_1936 are separated by 142 nucleotides Pf6N2E2_1936 and Pf6N2E2_1937 are separated by 68 nucleotides
Pf6N2E2_1934 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,290,755 to 2,291,549
_1934
Pf6N2E2_1935 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,291,613 to 2,292,257
_1935
Pf6N2E2_1936 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like, at 2,292,400 to 2,293,440
_1936
Pf6N2E2_1937 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 2,293,509 to 2,295,524
_1937
Position (kb)
2292
2293
2294 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 2291.500 kb on - strand at 2291.509 kb on - strand at 2291.529 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.790 kb on + strand, within Pf6N2E2_1935 at 2291.790 kb on + strand, within Pf6N2E2_1935 at 2291.791 kb on - strand, within Pf6N2E2_1935 at 2291.889 kb on + strand, within Pf6N2E2_1935 at 2291.890 kb on - strand, within Pf6N2E2_1935 at 2292.234 kb on - strand at 2292.234 kb on - strand at 2292.234 kb on - strand at 2292.281 kb on + strand at 2292.282 kb on - strand at 2292.282 kb on - strand at 2292.334 kb on - strand at 2292.401 kb on + strand at 2292.521 kb on + strand, within Pf6N2E2_1936 at 2292.522 kb on - strand, within Pf6N2E2_1936 at 2292.522 kb on - strand, within Pf6N2E2_1936 at 2292.595 kb on + strand, within Pf6N2E2_1936 at 2292.596 kb on - strand, within Pf6N2E2_1936 at 2292.596 kb on - strand, within Pf6N2E2_1936 at 2292.710 kb on + strand, within Pf6N2E2_1936 at 2292.710 kb on + strand, within Pf6N2E2_1936 at 2292.719 kb on - strand, within Pf6N2E2_1936 at 2292.814 kb on - strand, within Pf6N2E2_1936 at 2292.841 kb on + strand, within Pf6N2E2_1936 at 2292.901 kb on - strand, within Pf6N2E2_1936 at 2292.914 kb on + strand, within Pf6N2E2_1936 at 2292.981 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.008 kb on - strand, within Pf6N2E2_1936 at 2293.229 kb on - strand, within Pf6N2E2_1936 at 2293.270 kb on + strand, within Pf6N2E2_1936 at 2293.313 kb on + strand, within Pf6N2E2_1936 at 2293.313 kb on + strand, within Pf6N2E2_1936 at 2293.314 kb on - strand, within Pf6N2E2_1936 at 2293.351 kb on + strand at 2293.352 kb on - strand at 2293.352 kb on - strand at 2293.357 kb on - strand at 2293.441 kb on - strand at 2293.471 kb on - strand at 2293.471 kb on - strand at 2293.557 kb on + strand at 2293.558 kb on - strand at 2293.587 kb on + strand at 2293.694 kb on + strand at 2293.771 kb on - strand, within Pf6N2E2_1937 at 2293.980 kb on + strand, within Pf6N2E2_1937 at 2294.174 kb on + strand, within Pf6N2E2_1937 at 2294.196 kb on + strand, within Pf6N2E2_1937 at 2294.326 kb on - strand, within Pf6N2E2_1937
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 2,291,500 - +0.0 2,291,509 - +0.5 2,291,529 - -0.3 2,291,553 - -0.6 2,291,553 - +1.2 2,291,553 - +0.6 2,291,553 - -0.4 2,291,553 - -1.6 2,291,553 - +0.5 2,291,790 + Pf6N2E2_1935 0.27 +0.4 2,291,790 + Pf6N2E2_1935 0.27 +0.7 2,291,791 - Pf6N2E2_1935 0.28 +0.7 2,291,889 + Pf6N2E2_1935 0.43 -1.7 2,291,890 - Pf6N2E2_1935 0.43 -1.1 2,292,234 - -0.3 2,292,234 - -1.1 2,292,234 - -0.6 2,292,281 + +0.9 2,292,282 - +0.7 2,292,282 - -0.5 2,292,334 - -0.3 2,292,401 + -0.8 2,292,521 + Pf6N2E2_1936 0.12 +1.4 2,292,522 - Pf6N2E2_1936 0.12 -0.1 2,292,522 - Pf6N2E2_1936 0.12 +5.8 2,292,595 + Pf6N2E2_1936 0.19 -0.5 2,292,596 - Pf6N2E2_1936 0.19 +0.6 2,292,596 - Pf6N2E2_1936 0.19 +0.2 2,292,710 + Pf6N2E2_1936 0.30 -0.2 2,292,710 + Pf6N2E2_1936 0.30 +0.8 2,292,719 - Pf6N2E2_1936 0.31 +0.1 2,292,814 - Pf6N2E2_1936 0.40 +1.4 2,292,841 + Pf6N2E2_1936 0.42 -1.3 2,292,901 - Pf6N2E2_1936 0.48 +0.6 2,292,914 + Pf6N2E2_1936 0.49 -0.8 2,292,981 + Pf6N2E2_1936 0.56 +0.6 2,293,007 + Pf6N2E2_1936 0.58 +1.1 2,293,007 + Pf6N2E2_1936 0.58 +1.6 2,293,007 + Pf6N2E2_1936 0.58 +0.7 2,293,007 + Pf6N2E2_1936 0.58 +0.3 2,293,008 - Pf6N2E2_1936 0.58 -2.0 2,293,229 - Pf6N2E2_1936 0.80 -0.3 2,293,270 + Pf6N2E2_1936 0.84 +1.0 2,293,313 + Pf6N2E2_1936 0.88 -0.3 2,293,313 + Pf6N2E2_1936 0.88 +0.8 2,293,314 - Pf6N2E2_1936 0.88 -1.4 2,293,351 + +1.4 2,293,352 - +0.4 2,293,352 - +1.8 2,293,357 - +1.6 2,293,441 - +1.8 2,293,471 - -1.4 2,293,471 - -0.9 2,293,557 + -0.3 2,293,558 - -0.9 2,293,587 + +1.1 2,293,694 + +0.9 2,293,771 - Pf6N2E2_1937 0.13 -1.2 2,293,980 + Pf6N2E2_1937 0.23 -0.2 2,294,174 + Pf6N2E2_1937 0.33 -1.5 2,294,196 + Pf6N2E2_1937 0.34 -0.7 2,294,326 - Pf6N2E2_1937 0.41 +0.5
Or see this region's nucleotide sequence