Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1705

Experiment: D-Glucosamine Hydrochloride (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1703 and Pf6N2E2_1704 are separated by 4 nucleotidesPf6N2E2_1704 and Pf6N2E2_1705 overlap by 4 nucleotidesPf6N2E2_1705 and Pf6N2E2_1706 overlap by 4 nucleotidesPf6N2E2_1706 and Pf6N2E2_1707 are separated by 4 nucleotides Pf6N2E2_1703 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 2,011,840 to 2,012,904 _1703 Pf6N2E2_1704 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 2,012,909 to 2,013,790 _1704 Pf6N2E2_1705 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,013,787 to 2,014,557 _1705 Pf6N2E2_1706 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,014,554 to 2,015,525 _1706 Pf6N2E2_1707 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 2,015,530 to 2,016,915 _1707 Position (kb) 2013 2014 2015Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2012.815 kb on + strandat 2012.874 kb on + strandat 2012.875 kb on - strandat 2012.912 kb on + strandat 2012.958 kb on + strandat 2012.958 kb on + strandat 2012.958 kb on + strandat 2012.958 kb on + strandat 2012.958 kb on + strandat 2012.959 kb on - strandat 2012.982 kb on + strandat 2012.983 kb on - strandat 2013.027 kb on + strand, within Pf6N2E2_1704at 2013.514 kb on + strand, within Pf6N2E2_1704at 2013.705 kb on + strandat 2013.705 kb on + strandat 2013.705 kb on + strandat 2013.706 kb on - strandat 2013.706 kb on - strandat 2013.810 kb on - strandat 2013.875 kb on + strand, within Pf6N2E2_1705at 2014.573 kb on + strandat 2014.574 kb on - strandat 2014.574 kb on - strandat 2014.611 kb on + strandat 2015.187 kb on + strand, within Pf6N2E2_1706at 2015.199 kb on + strand, within Pf6N2E2_1706at 2015.199 kb on + strand, within Pf6N2E2_1706at 2015.199 kb on + strand, within Pf6N2E2_1706at 2015.200 kb on - strand, within Pf6N2E2_1706at 2015.515 kb on + strandat 2015.515 kb on + strandat 2015.516 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C)
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2,012,815 + +0.2
2,012,874 + +0.0
2,012,875 - +0.2
2,012,912 + -0.6
2,012,958 + -0.3
2,012,958 + +1.0
2,012,958 + +0.3
2,012,958 + +0.6
2,012,958 + -0.4
2,012,959 - +0.0
2,012,982 + +0.2
2,012,983 - +0.6
2,013,027 + Pf6N2E2_1704 0.13 -0.8
2,013,514 + Pf6N2E2_1704 0.69 +3.8
2,013,705 + -0.0
2,013,705 + +0.4
2,013,705 + -1.1
2,013,706 - -1.5
2,013,706 - -0.6
2,013,810 - -0.6
2,013,875 + Pf6N2E2_1705 0.11 -1.1
2,014,573 + -2.2
2,014,574 - -0.1
2,014,574 - -0.2
2,014,611 + +0.8
2,015,187 + Pf6N2E2_1706 0.65 -1.6
2,015,199 + Pf6N2E2_1706 0.66 -1.1
2,015,199 + Pf6N2E2_1706 0.66 +0.5
2,015,199 + Pf6N2E2_1706 0.66 +0.4
2,015,200 - Pf6N2E2_1706 0.66 +0.4
2,015,515 + +0.7
2,015,515 + -0.2
2,015,516 - +0.5

Or see this region's nucleotide sequence