Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1703 and Pf6N2E2_1704 are separated by 4 nucleotides Pf6N2E2_1704 and Pf6N2E2_1705 overlap by 4 nucleotides Pf6N2E2_1705 and Pf6N2E2_1706 overlap by 4 nucleotides Pf6N2E2_1706 and Pf6N2E2_1707 are separated by 4 nucleotides
Pf6N2E2_1703 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 2,011,840 to 2,012,904
_1703
Pf6N2E2_1704 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 2,012,909 to 2,013,790
_1704
Pf6N2E2_1705 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,013,787 to 2,014,557
_1705
Pf6N2E2_1706 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,014,554 to 2,015,525
_1706
Pf6N2E2_1707 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 2,015,530 to 2,016,915
_1707
Position (kb)
2013
2014
2015 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2012.815 kb on + strand at 2012.874 kb on + strand at 2012.875 kb on - strand at 2012.912 kb on + strand at 2012.958 kb on + strand at 2012.958 kb on + strand at 2012.958 kb on + strand at 2012.958 kb on + strand at 2012.958 kb on + strand at 2012.959 kb on - strand at 2012.982 kb on + strand at 2012.983 kb on - strand at 2013.027 kb on + strand, within Pf6N2E2_1704 at 2013.514 kb on + strand, within Pf6N2E2_1704 at 2013.705 kb on + strand at 2013.705 kb on + strand at 2013.705 kb on + strand at 2013.706 kb on - strand at 2013.706 kb on - strand at 2013.810 kb on - strand at 2013.875 kb on + strand, within Pf6N2E2_1705 at 2014.573 kb on + strand at 2014.574 kb on - strand at 2014.574 kb on - strand at 2014.611 kb on + strand at 2015.187 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.200 kb on - strand, within Pf6N2E2_1706 at 2015.515 kb on + strand at 2015.515 kb on + strand at 2015.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 2,012,815 + +0.2 2,012,874 + +0.0 2,012,875 - +0.2 2,012,912 + -0.6 2,012,958 + -0.3 2,012,958 + +1.0 2,012,958 + +0.3 2,012,958 + +0.6 2,012,958 + -0.4 2,012,959 - +0.0 2,012,982 + +0.2 2,012,983 - +0.6 2,013,027 + Pf6N2E2_1704 0.13 -0.8 2,013,514 + Pf6N2E2_1704 0.69 +3.8 2,013,705 + -0.0 2,013,705 + +0.4 2,013,705 + -1.1 2,013,706 - -1.5 2,013,706 - -0.6 2,013,810 - -0.6 2,013,875 + Pf6N2E2_1705 0.11 -1.1 2,014,573 + -2.2 2,014,574 - -0.1 2,014,574 - -0.2 2,014,611 + +0.8 2,015,187 + Pf6N2E2_1706 0.65 -1.6 2,015,199 + Pf6N2E2_1706 0.66 -1.1 2,015,199 + Pf6N2E2_1706 0.66 +0.5 2,015,199 + Pf6N2E2_1706 0.66 +0.4 2,015,200 - Pf6N2E2_1706 0.66 +0.4 2,015,515 + +0.7 2,015,515 + -0.2 2,015,516 - +0.5
Or see this region's nucleotide sequence