Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1441 and Pf6N2E2_1442 are separated by 120 nucleotides Pf6N2E2_1442 and Pf6N2E2_1443 are separated by 113 nucleotides
Pf6N2E2_1441 - Uncharacterized oxidoreductase ydgJ (EC 1.-.-.-), at 1,691,129 to 1,692,055
_1441
Pf6N2E2_1442 - Transcriptional regulators of sugar metabolism, at 1,692,176 to 1,692,970
_1442
Pf6N2E2_1443 - putative aldo/keto reductase, at 1,693,084 to 1,694,091
_1443
Position (kb)
1692
1693 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1691.251 kb on - strand, within Pf6N2E2_1441 at 1691.354 kb on + strand, within Pf6N2E2_1441 at 1691.354 kb on + strand, within Pf6N2E2_1441 at 1691.355 kb on - strand, within Pf6N2E2_1441 at 1692.079 kb on - strand at 1692.101 kb on - strand at 1692.139 kb on + strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.170 kb on - strand at 1692.182 kb on - strand at 1692.182 kb on - strand at 1692.182 kb on - strand at 1692.228 kb on + strand at 1692.228 kb on + strand at 1692.462 kb on + strand, within Pf6N2E2_1442 at 1692.463 kb on - strand, within Pf6N2E2_1442 at 1692.749 kb on + strand, within Pf6N2E2_1442 at 1692.750 kb on - strand, within Pf6N2E2_1442 at 1692.750 kb on - strand, within Pf6N2E2_1442 at 1692.750 kb on - strand, within Pf6N2E2_1442 at 1692.783 kb on - strand, within Pf6N2E2_1442 at 1692.795 kb on + strand, within Pf6N2E2_1442 at 1692.809 kb on + strand, within Pf6N2E2_1442 at 1693.029 kb on - strand at 1693.452 kb on - strand, within Pf6N2E2_1443 at 1693.452 kb on - strand, within Pf6N2E2_1443 at 1693.527 kb on - strand, within Pf6N2E2_1443 at 1693.573 kb on - strand, within Pf6N2E2_1443 at 1693.911 kb on - strand, within Pf6N2E2_1443 at 1693.911 kb on - strand, within Pf6N2E2_1443 at 1693.955 kb on + strand, within Pf6N2E2_1443 at 1693.955 kb on + strand, within Pf6N2E2_1443 at 1693.955 kb on + strand, within Pf6N2E2_1443
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 1,691,251 - Pf6N2E2_1441 0.13 +1.6 1,691,354 + Pf6N2E2_1441 0.24 +0.5 1,691,354 + Pf6N2E2_1441 0.24 +0.2 1,691,355 - Pf6N2E2_1441 0.24 -0.8 1,692,079 - -0.7 1,692,101 - -1.2 1,692,139 + +0.1 1,692,140 - -0.7 1,692,140 - -1.0 1,692,140 - +0.4 1,692,140 - +0.9 1,692,140 - -1.1 1,692,140 - +0.2 1,692,170 - +0.8 1,692,182 - -0.7 1,692,182 - -3.1 1,692,182 - -0.4 1,692,228 + +3.1 1,692,228 + +3.3 1,692,462 + Pf6N2E2_1442 0.36 +3.1 1,692,463 - Pf6N2E2_1442 0.36 +2.9 1,692,749 + Pf6N2E2_1442 0.72 +2.6 1,692,750 - Pf6N2E2_1442 0.72 +2.9 1,692,750 - Pf6N2E2_1442 0.72 +3.2 1,692,750 - Pf6N2E2_1442 0.72 +3.1 1,692,783 - Pf6N2E2_1442 0.76 +3.6 1,692,795 + Pf6N2E2_1442 0.78 +2.9 1,692,809 + Pf6N2E2_1442 0.80 +2.8 1,693,029 - +3.1 1,693,452 - Pf6N2E2_1443 0.37 +0.6 1,693,452 - Pf6N2E2_1443 0.37 -0.1 1,693,527 - Pf6N2E2_1443 0.44 -0.7 1,693,573 - Pf6N2E2_1443 0.49 -0.6 1,693,911 - Pf6N2E2_1443 0.82 -0.7 1,693,911 - Pf6N2E2_1443 0.82 -1.0 1,693,955 + Pf6N2E2_1443 0.86 -0.7 1,693,955 + Pf6N2E2_1443 0.86 +0.7 1,693,955 + Pf6N2E2_1443 0.86 +0.0
Or see this region's nucleotide sequence