Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1398 and Pf6N2E2_1399 are separated by 54 nucleotides Pf6N2E2_1399 and Pf6N2E2_1400 are separated by 159 nucleotides Pf6N2E2_1400 and Pf6N2E2_1401 overlap by 32 nucleotides
Pf6N2E2_1398 - Rhodanese-related sulfurtransferase, at 1,638,215 to 1,638,748
_1398
Pf6N2E2_1399 - Transcriptional regulator, LuxR family, at 1,638,803 to 1,639,474
_1399
Pf6N2E2_1400 - Sensory box histidine kinase/response regulator, at 1,639,634 to 1,642,234
_1400
Pf6N2E2_1401 - FIG00955631: hypothetical protein, at 1,642,203 to 1,643,366
_1401
Position (kb)
1639
1640
1641
1642
1643 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 1638.634 kb on - strand, within Pf6N2E2_1398 at 1638.657 kb on - strand, within Pf6N2E2_1398 at 1638.657 kb on - strand, within Pf6N2E2_1398 at 1638.668 kb on + strand, within Pf6N2E2_1398 at 1638.668 kb on + strand, within Pf6N2E2_1398 at 1638.792 kb on + strand at 1638.812 kb on + strand at 1638.812 kb on + strand at 1638.813 kb on - strand at 1638.813 kb on - strand at 1638.814 kb on + strand at 1639.097 kb on + strand, within Pf6N2E2_1399 at 1639.098 kb on - strand, within Pf6N2E2_1399 at 1639.098 kb on - strand, within Pf6N2E2_1399 at 1639.181 kb on + strand, within Pf6N2E2_1399 at 1639.181 kb on + strand, within Pf6N2E2_1399 at 1639.181 kb on + strand, within Pf6N2E2_1399 at 1639.181 kb on + strand, within Pf6N2E2_1399 at 1639.182 kb on - strand, within Pf6N2E2_1399 at 1639.182 kb on - strand, within Pf6N2E2_1399 at 1639.460 kb on + strand at 1639.460 kb on + strand at 1639.460 kb on + strand at 1639.753 kb on - strand at 1639.753 kb on - strand at 1639.881 kb on + strand at 1639.881 kb on + strand at 1639.881 kb on + strand at 1639.881 kb on + strand at 1639.881 kb on + strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.957 kb on + strand, within Pf6N2E2_1400 at 1639.957 kb on + strand, within Pf6N2E2_1400 at 1639.957 kb on + strand, within Pf6N2E2_1400 at 1639.957 kb on + strand, within Pf6N2E2_1400 at 1639.958 kb on - strand, within Pf6N2E2_1400 at 1639.958 kb on - strand, within Pf6N2E2_1400 at 1639.958 kb on - strand, within Pf6N2E2_1400 at 1640.240 kb on + strand, within Pf6N2E2_1400 at 1640.240 kb on + strand, within Pf6N2E2_1400 at 1640.267 kb on - strand, within Pf6N2E2_1400 at 1640.473 kb on - strand at 1640.530 kb on + strand, within Pf6N2E2_1400 at 1640.531 kb on - strand, within Pf6N2E2_1400 at 1640.975 kb on + strand, within Pf6N2E2_1400 at 1640.976 kb on - strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.980 kb on - strand, within Pf6N2E2_1400 at 1640.980 kb on - strand, within Pf6N2E2_1400 at 1640.980 kb on - strand, within Pf6N2E2_1400 at 1640.994 kb on + strand, within Pf6N2E2_1400 at 1640.994 kb on + strand, within Pf6N2E2_1400 at 1640.995 kb on - strand, within Pf6N2E2_1400 at 1640.995 kb on - strand, within Pf6N2E2_1400 at 1640.995 kb on - strand, within Pf6N2E2_1400 at 1641.069 kb on + strand, within Pf6N2E2_1400 at 1641.069 kb on + strand, within Pf6N2E2_1400 at 1641.069 kb on + strand, within Pf6N2E2_1400 at 1641.069 kb on + strand, within Pf6N2E2_1400 at 1641.070 kb on - strand, within Pf6N2E2_1400 at 1641.150 kb on + strand, within Pf6N2E2_1400 at 1641.150 kb on + strand, within Pf6N2E2_1400 at 1641.150 kb on + strand, within Pf6N2E2_1400 at 1641.150 kb on + strand, within Pf6N2E2_1400 at 1641.150 kb on - strand, within Pf6N2E2_1400 at 1641.151 kb on - strand, within Pf6N2E2_1400 at 1641.163 kb on - strand, within Pf6N2E2_1400 at 1641.204 kb on + strand, within Pf6N2E2_1400 at 1641.661 kb on + strand, within Pf6N2E2_1400 at 1641.735 kb on + strand, within Pf6N2E2_1400 at 1641.736 kb on - strand, within Pf6N2E2_1400 at 1642.112 kb on + strand at 1642.262 kb on + strand at 1642.263 kb on - strand at 1642.288 kb on + strand at 1642.288 kb on + strand at 1642.289 kb on - strand at 1642.289 kb on - strand at 1642.511 kb on - strand, within Pf6N2E2_1401 at 1642.511 kb on - strand, within Pf6N2E2_1401 at 1642.511 kb on - strand, within Pf6N2E2_1401 at 1642.511 kb on - strand, within Pf6N2E2_1401 at 1642.511 kb on - strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.559 kb on - strand, within Pf6N2E2_1401 at 1642.559 kb on - strand, within Pf6N2E2_1401 at 1642.561 kb on + strand, within Pf6N2E2_1401 at 1642.648 kb on + strand, within Pf6N2E2_1401 at 1642.648 kb on + strand, within Pf6N2E2_1401 at 1642.649 kb on - strand, within Pf6N2E2_1401 at 1642.682 kb on - strand, within Pf6N2E2_1401 at 1642.829 kb on - strand, within Pf6N2E2_1401 at 1642.849 kb on + strand, within Pf6N2E2_1401 at 1642.850 kb on - strand, within Pf6N2E2_1401 at 1642.850 kb on - strand, within Pf6N2E2_1401 at 1643.230 kb on + strand, within Pf6N2E2_1401 at 1643.231 kb on - strand, within Pf6N2E2_1401
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 1,638,634 - Pf6N2E2_1398 0.78 +9.9 1,638,657 - Pf6N2E2_1398 0.83 +8.4 1,638,657 - Pf6N2E2_1398 0.83 +9.0 1,638,668 + Pf6N2E2_1398 0.85 +1.2 1,638,668 + Pf6N2E2_1398 0.85 +2.6 1,638,792 + +5.7 1,638,812 + +8.1 1,638,812 + +9.3 1,638,813 - +9.5 1,638,813 - +8.9 1,638,814 + +10.1 1,639,097 + Pf6N2E2_1399 0.44 +9.7 1,639,098 - Pf6N2E2_1399 0.44 +8.5 1,639,098 - Pf6N2E2_1399 0.44 +8.8 1,639,181 + Pf6N2E2_1399 0.56 +8.5 1,639,181 + Pf6N2E2_1399 0.56 +8.8 1,639,181 + Pf6N2E2_1399 0.56 +8.4 1,639,181 + Pf6N2E2_1399 0.56 +9.1 1,639,182 - Pf6N2E2_1399 0.56 +8.9 1,639,182 - Pf6N2E2_1399 0.56 +8.8 1,639,460 + +4.3 1,639,460 + +4.4 1,639,460 + +4.7 1,639,753 - -2.6 1,639,753 - -2.7 1,639,881 + -1.4 1,639,881 + -2.1 1,639,881 + -3.5 1,639,881 + -3.4 1,639,881 + -1.9 1,639,882 - -3.7 1,639,882 - -2.1 1,639,882 - -2.0 1,639,882 - -2.1 1,639,882 - -1.4 1,639,882 - -1.6 1,639,882 - -1.9 1,639,957 + Pf6N2E2_1400 0.12 +1.6 1,639,957 + Pf6N2E2_1400 0.12 -3.3 1,639,957 + Pf6N2E2_1400 0.12 +0.0 1,639,957 + Pf6N2E2_1400 0.12 +0.0 1,639,958 - Pf6N2E2_1400 0.12 -3.3 1,639,958 - Pf6N2E2_1400 0.12 -1.9 1,639,958 - Pf6N2E2_1400 0.12 -4.2 1,640,240 + Pf6N2E2_1400 0.23 -1.5 1,640,240 + Pf6N2E2_1400 0.23 -2.1 1,640,267 - Pf6N2E2_1400 0.24 -1.7 1,640,473 - +1.6 1,640,530 + Pf6N2E2_1400 0.34 -3.4 1,640,531 - Pf6N2E2_1400 0.34 -3.0 1,640,975 + Pf6N2E2_1400 0.52 -3.3 1,640,976 - Pf6N2E2_1400 0.52 -0.4 1,640,979 + Pf6N2E2_1400 0.52 -2.7 1,640,979 + Pf6N2E2_1400 0.52 -2.6 1,640,979 + Pf6N2E2_1400 0.52 -3.3 1,640,979 + Pf6N2E2_1400 0.52 -2.5 1,640,979 + Pf6N2E2_1400 0.52 -2.5 1,640,979 + Pf6N2E2_1400 0.52 -1.9 1,640,980 - Pf6N2E2_1400 0.52 -1.4 1,640,980 - Pf6N2E2_1400 0.52 -2.9 1,640,980 - Pf6N2E2_1400 0.52 -1.7 1,640,994 + Pf6N2E2_1400 0.52 -3.2 1,640,994 + Pf6N2E2_1400 0.52 -0.9 1,640,995 - Pf6N2E2_1400 0.52 -1.2 1,640,995 - Pf6N2E2_1400 0.52 -1.9 1,640,995 - Pf6N2E2_1400 0.52 -1.7 1,641,069 + Pf6N2E2_1400 0.55 -3.5 1,641,069 + Pf6N2E2_1400 0.55 -0.2 1,641,069 + Pf6N2E2_1400 0.55 +0.5 1,641,069 + Pf6N2E2_1400 0.55 -1.4 1,641,070 - Pf6N2E2_1400 0.55 -1.5 1,641,150 + Pf6N2E2_1400 0.58 -1.9 1,641,150 + Pf6N2E2_1400 0.58 -2.2 1,641,150 + Pf6N2E2_1400 0.58 -3.2 1,641,150 + Pf6N2E2_1400 0.58 -3.3 1,641,150 - Pf6N2E2_1400 0.58 -2.7 1,641,151 - Pf6N2E2_1400 0.58 -1.6 1,641,163 - Pf6N2E2_1400 0.59 -3.6 1,641,204 + Pf6N2E2_1400 0.60 -0.4 1,641,661 + Pf6N2E2_1400 0.78 -1.4 1,641,735 + Pf6N2E2_1400 0.81 -2.3 1,641,736 - Pf6N2E2_1400 0.81 -1.2 1,642,112 + -0.7 1,642,262 + -2.6 1,642,263 - +4.8 1,642,288 + +0.8 1,642,288 + -3.3 1,642,289 - +5.5 1,642,289 - +6.0 1,642,511 - Pf6N2E2_1401 0.26 +0.2 1,642,511 - Pf6N2E2_1401 0.26 -3.3 1,642,511 - Pf6N2E2_1401 0.26 -1.6 1,642,511 - Pf6N2E2_1401 0.26 -0.7 1,642,511 - Pf6N2E2_1401 0.26 -3.0 1,642,558 + Pf6N2E2_1401 0.30 -2.5 1,642,558 + Pf6N2E2_1401 0.30 -2.0 1,642,558 + Pf6N2E2_1401 0.30 -0.8 1,642,558 + Pf6N2E2_1401 0.30 -2.1 1,642,558 + Pf6N2E2_1401 0.30 -2.5 1,642,558 + Pf6N2E2_1401 0.30 -2.7 1,642,559 - Pf6N2E2_1401 0.31 -1.7 1,642,559 - Pf6N2E2_1401 0.31 -2.5 1,642,561 + Pf6N2E2_1401 0.31 -2.0 1,642,648 + Pf6N2E2_1401 0.38 -1.9 1,642,648 + Pf6N2E2_1401 0.38 -4.0 1,642,649 - Pf6N2E2_1401 0.38 -2.5 1,642,682 - Pf6N2E2_1401 0.41 -1.2 1,642,829 - Pf6N2E2_1401 0.54 -2.6 1,642,849 + Pf6N2E2_1401 0.55 -3.1 1,642,850 - Pf6N2E2_1401 0.56 -1.7 1,642,850 - Pf6N2E2_1401 0.56 -2.4 1,643,230 + Pf6N2E2_1401 0.88 -1.2 1,643,231 - Pf6N2E2_1401 0.88 -2.3
Or see this region's nucleotide sequence