Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23465 and CA265_RS23470 are separated by 92 nucleotides CA265_RS23470 and CA265_RS23475 are separated by 15 nucleotides
CA265_RS23465: CA265_RS23465 - threonine synthase, at 5,739,455 to 5,740,762
_RS23465
CA265_RS23470: CA265_RS23470 - homoserine kinase (EC 2.7.1.39) (from data) , at 5,740,855 to 5,741,787
_RS23470
CA265_RS23475: CA265_RS23475 - aspartate kinase; homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3) (from data) , at 5,741,803 to 5,744,256
_RS23475
Position (kb)
5740
5741
5742 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5739.932 kb on - strand, within CA265_RS23465 at 5739.940 kb on + strand, within CA265_RS23465 at 5739.941 kb on - strand, within CA265_RS23465 at 5739.965 kb on + strand, within CA265_RS23465 at 5740.001 kb on - strand, within CA265_RS23465 at 5740.068 kb on + strand, within CA265_RS23465 at 5740.144 kb on + strand, within CA265_RS23465 at 5740.292 kb on + strand, within CA265_RS23465 at 5740.292 kb on + strand, within CA265_RS23465 at 5740.314 kb on - strand, within CA265_RS23465 at 5740.404 kb on + strand, within CA265_RS23465 at 5740.483 kb on - strand, within CA265_RS23465 at 5740.631 kb on - strand, within CA265_RS23465 at 5740.672 kb on + strand at 5740.672 kb on + strand at 5740.672 kb on + strand at 5740.672 kb on + strand at 5740.673 kb on - strand at 5740.673 kb on - strand at 5740.673 kb on - strand at 5740.674 kb on + strand at 5740.674 kb on + strand at 5740.674 kb on + strand at 5740.674 kb on + strand at 5740.675 kb on - strand at 5740.675 kb on - strand at 5740.677 kb on - strand at 5740.699 kb on + strand at 5740.700 kb on - strand at 5740.754 kb on + strand at 5740.755 kb on - strand at 5740.831 kb on - strand at 5740.839 kb on + strand at 5740.853 kb on - strand at 5740.857 kb on - strand at 5741.092 kb on + strand, within CA265_RS23470 at 5741.139 kb on + strand, within CA265_RS23470 at 5741.140 kb on - strand, within CA265_RS23470 at 5741.239 kb on + strand, within CA265_RS23470 at 5741.270 kb on + strand, within CA265_RS23470 at 5741.290 kb on + strand, within CA265_RS23470 at 5741.299 kb on + strand, within CA265_RS23470 at 5741.300 kb on - strand, within CA265_RS23470 at 5741.316 kb on - strand, within CA265_RS23470 at 5741.341 kb on + strand, within CA265_RS23470 at 5741.435 kb on - strand, within CA265_RS23470 at 5741.435 kb on - strand, within CA265_RS23470 at 5741.546 kb on - strand, within CA265_RS23470 at 5741.546 kb on - strand, within CA265_RS23470 at 5741.546 kb on - strand, within CA265_RS23470 at 5741.552 kb on - strand, within CA265_RS23470 at 5741.569 kb on + strand, within CA265_RS23470 at 5741.569 kb on + strand, within CA265_RS23470 at 5741.582 kb on - strand, within CA265_RS23470 at 5741.594 kb on - strand, within CA265_RS23470 at 5741.722 kb on + strand at 5741.722 kb on + strand at 5741.741 kb on - strand at 5741.741 kb on - strand at 5741.778 kb on + strand at 5741.817 kb on + strand at 5741.821 kb on + strand at 5741.832 kb on + strand at 5741.905 kb on - strand at 5741.928 kb on + strand at 5741.929 kb on - strand at 5741.932 kb on - strand at 5741.932 kb on - strand at 5741.948 kb on - strand at 5741.953 kb on - strand at 5741.996 kb on - strand at 5742.001 kb on - strand at 5742.085 kb on - strand, within CA265_RS23475 at 5742.214 kb on - strand, within CA265_RS23475 at 5742.220 kb on - strand, within CA265_RS23475 at 5742.261 kb on + strand, within CA265_RS23475 at 5742.262 kb on - strand, within CA265_RS23475 at 5742.370 kb on + strand, within CA265_RS23475 at 5742.376 kb on - strand, within CA265_RS23475 at 5742.430 kb on + strand, within CA265_RS23475 at 5742.431 kb on - strand, within CA265_RS23475 at 5742.443 kb on + strand, within CA265_RS23475 at 5742.444 kb on - strand, within CA265_RS23475 at 5742.489 kb on + strand, within CA265_RS23475 at 5742.678 kb on + strand, within CA265_RS23475 at 5742.741 kb on + strand, within CA265_RS23475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 5,739,932 - CA265_RS23465 0.36 +0.5 5,739,940 + CA265_RS23465 0.37 +0.4 5,739,941 - CA265_RS23465 0.37 -1.1 5,739,965 + CA265_RS23465 0.39 -0.7 5,740,001 - CA265_RS23465 0.42 -0.0 5,740,068 + CA265_RS23465 0.47 +1.0 5,740,144 + CA265_RS23465 0.53 +0.4 5,740,292 + CA265_RS23465 0.64 +1.2 5,740,292 + CA265_RS23465 0.64 +0.7 5,740,314 - CA265_RS23465 0.66 -0.3 5,740,404 + CA265_RS23465 0.73 +0.4 5,740,483 - CA265_RS23465 0.79 -3.4 5,740,631 - CA265_RS23465 0.90 +1.0 5,740,672 + -0.1 5,740,672 + -1.2 5,740,672 + -0.0 5,740,672 + +1.3 5,740,673 - -1.2 5,740,673 - +0.4 5,740,673 - +0.3 5,740,674 + +1.2 5,740,674 + +0.8 5,740,674 + +2.0 5,740,674 + -0.3 5,740,675 - -0.0 5,740,675 - -0.1 5,740,677 - +0.2 5,740,699 + +0.3 5,740,700 - +0.6 5,740,754 + -0.8 5,740,755 - +0.2 5,740,831 - -0.2 5,740,839 + +0.3 5,740,853 - -1.3 5,740,857 - -0.8 5,741,092 + CA265_RS23470 0.25 +0.6 5,741,139 + CA265_RS23470 0.30 -0.9 5,741,140 - CA265_RS23470 0.31 +0.4 5,741,239 + CA265_RS23470 0.41 +0.6 5,741,270 + CA265_RS23470 0.44 -0.9 5,741,290 + CA265_RS23470 0.47 +0.5 5,741,299 + CA265_RS23470 0.48 +0.8 5,741,300 - CA265_RS23470 0.48 +0.8 5,741,316 - CA265_RS23470 0.49 -0.5 5,741,341 + CA265_RS23470 0.52 +0.6 5,741,435 - CA265_RS23470 0.62 -0.3 5,741,435 - CA265_RS23470 0.62 -0.2 5,741,546 - CA265_RS23470 0.74 -0.1 5,741,546 - CA265_RS23470 0.74 -0.6 5,741,546 - CA265_RS23470 0.74 +0.1 5,741,552 - CA265_RS23470 0.75 +0.3 5,741,569 + CA265_RS23470 0.77 +0.8 5,741,569 + CA265_RS23470 0.77 -2.0 5,741,582 - CA265_RS23470 0.78 +1.1 5,741,594 - CA265_RS23470 0.79 -0.2 5,741,722 + +0.1 5,741,722 + -0.2 5,741,741 - -0.0 5,741,741 - -1.5 5,741,778 + +0.4 5,741,817 + -0.4 5,741,821 + -0.4 5,741,832 + -0.2 5,741,905 - -1.8 5,741,928 + -0.3 5,741,929 - +0.3 5,741,932 - -1.0 5,741,932 - +0.9 5,741,948 - -0.3 5,741,953 - +0.5 5,741,996 - +0.4 5,742,001 - -0.9 5,742,085 - CA265_RS23475 0.11 -1.5 5,742,214 - CA265_RS23475 0.17 -0.1 5,742,220 - CA265_RS23475 0.17 -1.0 5,742,261 + CA265_RS23475 0.19 -0.6 5,742,262 - CA265_RS23475 0.19 -1.5 5,742,370 + CA265_RS23475 0.23 +0.8 5,742,376 - CA265_RS23475 0.23 -0.6 5,742,430 + CA265_RS23475 0.26 -0.5 5,742,431 - CA265_RS23475 0.26 -0.2 5,742,443 + CA265_RS23475 0.26 +0.2 5,742,444 - CA265_RS23475 0.26 -1.4 5,742,489 + CA265_RS23475 0.28 +0.2 5,742,678 + CA265_RS23475 0.36 -1.1 5,742,741 + CA265_RS23475 0.38 +1.0
Or see this region's nucleotide sequence