Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22625 and CA265_RS22630 are separated by 71 nucleotides CA265_RS22630 and CA265_RS22635 overlap by 1 nucleotides
CA265_RS22625: CA265_RS22625 - arginase, at 5,540,018 to 5,541,199
_RS22625
CA265_RS22630: CA265_RS22630 - nucleoside-diphosphate sugar epimerase, at 5,541,271 to 5,542,233
_RS22630
CA265_RS22635: CA265_RS22635 - phosphoserine phosphatase (EC 3.1.3.3) (from data) , at 5,542,233 to 5,543,534
_RS22635
Position (kb)
5541
5542
5543 Strain fitness (log2 ratio)
-2
-1
0
1 at 5540.379 kb on - strand, within CA265_RS22625 at 5541.071 kb on - strand, within CA265_RS22625 at 5541.314 kb on + strand at 5541.497 kb on + strand, within CA265_RS22630 at 5541.542 kb on + strand, within CA265_RS22630 at 5541.542 kb on + strand, within CA265_RS22630 at 5541.830 kb on + strand, within CA265_RS22630 at 5541.831 kb on - strand, within CA265_RS22630 at 5541.852 kb on + strand, within CA265_RS22630 at 5542.130 kb on + strand, within CA265_RS22630 at 5542.137 kb on - strand at 5542.144 kb on + strand at 5542.155 kb on + strand at 5542.231 kb on + strand at 5542.232 kb on - strand at 5542.370 kb on + strand, within CA265_RS22635 at 5542.546 kb on + strand, within CA265_RS22635 at 5542.616 kb on + strand, within CA265_RS22635 at 5542.724 kb on - strand, within CA265_RS22635 at 5543.079 kb on - strand, within CA265_RS22635 at 5543.128 kb on + strand, within CA265_RS22635
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 5,540,379 - CA265_RS22625 0.31 +0.5 5,541,071 - CA265_RS22625 0.89 +0.5 5,541,314 + -1.7 5,541,497 + CA265_RS22630 0.23 -0.6 5,541,542 + CA265_RS22630 0.28 -0.3 5,541,542 + CA265_RS22630 0.28 -0.1 5,541,830 + CA265_RS22630 0.58 -0.6 5,541,831 - CA265_RS22630 0.58 +0.5 5,541,852 + CA265_RS22630 0.60 -0.7 5,542,130 + CA265_RS22630 0.89 -0.3 5,542,137 - -1.9 5,542,144 + +0.3 5,542,155 + -0.4 5,542,231 + -0.4 5,542,232 - +1.5 5,542,370 + CA265_RS22635 0.11 +0.0 5,542,546 + CA265_RS22635 0.24 -0.9 5,542,616 + CA265_RS22635 0.29 +0.5 5,542,724 - CA265_RS22635 0.38 +0.6 5,543,079 - CA265_RS22635 0.65 +1.3 5,543,128 + CA265_RS22635 0.69 +0.8
Or see this region's nucleotide sequence