Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20640 and CA265_RS20645 are separated by 212 nucleotides CA265_RS20645 and CA265_RS20650 are separated by 150 nucleotides CA265_RS20650 and CA265_RS20655 are separated by 75 nucleotides CA265_RS20655 and CA265_RS20660 are separated by 91 nucleotides
CA265_RS20640: CA265_RS20640 - hypothetical protein, at 5,109,208 to 5,110,335
_RS20640
CA265_RS20645: CA265_RS20645 - RNA polymerase subunit sigma-70, at 5,110,548 to 5,111,126
_RS20645
CA265_RS20650: CA265_RS20650 - hypothetical protein, at 5,111,277 to 5,111,528
_RS20650
CA265_RS20655: CA265_RS20655 - transcriptional regulator, at 5,111,604 to 5,111,873
_RS20655
CA265_RS20660: CA265_RS20660 - hypothetical protein, at 5,111,965 to 5,112,837
_RS20660
Position (kb)
5111
5112 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5110.337 kb on + strand at 5110.352 kb on + strand at 5110.374 kb on - strand at 5110.378 kb on - strand at 5110.414 kb on + strand at 5110.519 kb on + strand at 5110.561 kb on - strand at 5110.565 kb on + strand at 5110.565 kb on + strand at 5110.565 kb on + strand at 5110.570 kb on - strand at 5110.575 kb on + strand at 5110.619 kb on + strand, within CA265_RS20645 at 5110.623 kb on - strand, within CA265_RS20645 at 5110.633 kb on - strand, within CA265_RS20645 at 5110.649 kb on + strand, within CA265_RS20645 at 5110.708 kb on - strand, within CA265_RS20645 at 5110.708 kb on - strand, within CA265_RS20645 at 5110.731 kb on + strand, within CA265_RS20645 at 5110.737 kb on + strand, within CA265_RS20645 at 5110.737 kb on + strand, within CA265_RS20645 at 5110.880 kb on - strand, within CA265_RS20645 at 5110.900 kb on + strand, within CA265_RS20645 at 5110.923 kb on - strand, within CA265_RS20645 at 5110.954 kb on - strand, within CA265_RS20645 at 5110.958 kb on - strand, within CA265_RS20645 at 5110.958 kb on - strand, within CA265_RS20645 at 5111.031 kb on - strand, within CA265_RS20645 at 5111.035 kb on + strand, within CA265_RS20645 at 5111.035 kb on + strand, within CA265_RS20645 at 5111.089 kb on + strand at 5111.112 kb on - strand at 5111.112 kb on - strand at 5111.114 kb on + strand at 5111.116 kb on + strand at 5111.116 kb on + strand at 5111.191 kb on + strand at 5111.218 kb on - strand at 5111.234 kb on - strand at 5111.237 kb on + strand at 5111.352 kb on + strand, within CA265_RS20650 at 5111.426 kb on + strand, within CA265_RS20650 at 5111.504 kb on - strand at 5111.506 kb on - strand at 5111.548 kb on - strand at 5111.558 kb on - strand at 5111.579 kb on + strand at 5111.580 kb on - strand at 5111.603 kb on + strand at 5111.610 kb on - strand at 5111.718 kb on + strand, within CA265_RS20655 at 5111.724 kb on - strand, within CA265_RS20655 at 5111.724 kb on - strand, within CA265_RS20655 at 5111.733 kb on + strand, within CA265_RS20655 at 5111.769 kb on + strand, within CA265_RS20655 at 5111.769 kb on + strand, within CA265_RS20655 at 5111.770 kb on - strand, within CA265_RS20655 at 5111.770 kb on - strand, within CA265_RS20655 at 5111.770 kb on - strand, within CA265_RS20655 at 5111.770 kb on - strand, within CA265_RS20655 at 5111.789 kb on - strand, within CA265_RS20655 at 5111.801 kb on + strand, within CA265_RS20655 at 5111.801 kb on + strand, within CA265_RS20655 at 5111.868 kb on - strand at 5111.959 kb on + strand at 5111.963 kb on + strand at 5111.964 kb on - strand at 5111.978 kb on - strand at 5111.978 kb on - strand at 5111.992 kb on + strand at 5112.025 kb on + strand at 5112.025 kb on + strand at 5112.025 kb on + strand at 5112.026 kb on - strand at 5112.245 kb on - strand, within CA265_RS20660 at 5112.248 kb on - strand, within CA265_RS20660 at 5112.307 kb on + strand, within CA265_RS20660 at 5112.387 kb on - strand, within CA265_RS20660 at 5112.421 kb on + strand, within CA265_RS20660 at 5112.422 kb on - strand, within CA265_RS20660 at 5112.490 kb on + strand, within CA265_RS20660 at 5112.491 kb on - strand, within CA265_RS20660 at 5112.491 kb on - strand, within CA265_RS20660 at 5112.494 kb on + strand, within CA265_RS20660 at 5112.495 kb on - strand, within CA265_RS20660
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 5,110,337 + +0.5 5,110,352 + -0.5 5,110,374 - -1.3 5,110,378 - +0.9 5,110,414 + -0.6 5,110,519 + -0.2 5,110,561 - +0.3 5,110,565 + +0.9 5,110,565 + -1.7 5,110,565 + -0.7 5,110,570 - +0.1 5,110,575 + -0.0 5,110,619 + CA265_RS20645 0.12 -0.1 5,110,623 - CA265_RS20645 0.13 -0.1 5,110,633 - CA265_RS20645 0.15 -1.2 5,110,649 + CA265_RS20645 0.17 +0.1 5,110,708 - CA265_RS20645 0.28 -1.0 5,110,708 - CA265_RS20645 0.28 +0.7 5,110,731 + CA265_RS20645 0.32 +0.2 5,110,737 + CA265_RS20645 0.33 -0.2 5,110,737 + CA265_RS20645 0.33 +0.9 5,110,880 - CA265_RS20645 0.57 +1.8 5,110,900 + CA265_RS20645 0.61 +1.1 5,110,923 - CA265_RS20645 0.65 -0.6 5,110,954 - CA265_RS20645 0.70 +0.2 5,110,958 - CA265_RS20645 0.71 +0.2 5,110,958 - CA265_RS20645 0.71 -0.6 5,111,031 - CA265_RS20645 0.83 +0.1 5,111,035 + CA265_RS20645 0.84 +0.4 5,111,035 + CA265_RS20645 0.84 +0.4 5,111,089 + +1.2 5,111,112 - +0.1 5,111,112 - +1.5 5,111,114 + +1.5 5,111,116 + -0.6 5,111,116 + -0.2 5,111,191 + +0.1 5,111,218 - +0.3 5,111,234 - +0.5 5,111,237 + +1.5 5,111,352 + CA265_RS20650 0.30 +0.2 5,111,426 + CA265_RS20650 0.59 +1.1 5,111,504 - +2.7 5,111,506 - +0.2 5,111,548 - +0.9 5,111,558 - -2.9 5,111,579 + +0.2 5,111,580 - +0.7 5,111,603 + +0.4 5,111,610 - +0.1 5,111,718 + CA265_RS20655 0.42 -0.4 5,111,724 - CA265_RS20655 0.44 +0.6 5,111,724 - CA265_RS20655 0.44 -1.7 5,111,733 + CA265_RS20655 0.48 -0.3 5,111,769 + CA265_RS20655 0.61 +0.4 5,111,769 + CA265_RS20655 0.61 -0.5 5,111,770 - CA265_RS20655 0.61 -0.2 5,111,770 - CA265_RS20655 0.61 -0.0 5,111,770 - CA265_RS20655 0.61 -0.6 5,111,770 - CA265_RS20655 0.61 +0.3 5,111,789 - CA265_RS20655 0.69 +0.5 5,111,801 + CA265_RS20655 0.73 +0.1 5,111,801 + CA265_RS20655 0.73 +0.4 5,111,868 - +0.1 5,111,959 + -1.3 5,111,963 + +2.7 5,111,964 - +0.1 5,111,978 - +0.1 5,111,978 - +1.2 5,111,992 + -0.1 5,112,025 + +0.7 5,112,025 + -0.7 5,112,025 + +0.3 5,112,026 - -0.1 5,112,245 - CA265_RS20660 0.32 -0.0 5,112,248 - CA265_RS20660 0.32 +0.1 5,112,307 + CA265_RS20660 0.39 -0.9 5,112,387 - CA265_RS20660 0.48 -0.0 5,112,421 + CA265_RS20660 0.52 +0.1 5,112,422 - CA265_RS20660 0.52 -0.2 5,112,490 + CA265_RS20660 0.60 +0.7 5,112,491 - CA265_RS20660 0.60 +1.3 5,112,491 - CA265_RS20660 0.60 +0.9 5,112,494 + CA265_RS20660 0.61 -0.4 5,112,495 - CA265_RS20660 0.61 -0.0
Or see this region's nucleotide sequence