Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20255 and CA265_RS20260 are separated by 100 nucleotides CA265_RS20260 and CA265_RS20265 overlap by 9 nucleotides CA265_RS20265 and CA265_RS20270 are separated by 331 nucleotides CA265_RS20270 and CA265_RS20275 are separated by 40 nucleotides CA265_RS20275 and CA265_RS20280 are separated by 1 nucleotides
CA265_RS20255: CA265_RS20255 - trans-aconitate 2-methyltransferase, at 5,004,118 to 5,004,924
_RS20255
CA265_RS20260: CA265_RS20260 - tRNA-Arg, at 5,005,025 to 5,005,100
_RS20260
CA265_RS20265: CA265_RS20265 - excinuclease ABC subunit C, at 5,005,092 to 5,005,337
_RS20265
CA265_RS20270: CA265_RS20270 - methionine--tRNA ligase, at 5,005,669 to 5,007,777
_RS20270
CA265_RS20275: CA265_RS20275 - LD-carboxypeptidase, at 5,007,818 to 5,008,717
_RS20275
CA265_RS20280: CA265_RS20280 - DoxX family protein, at 5,008,719 to 5,009,126
_RS20280
Position (kb) 5005
5006
5007
5008 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5004.673 kb on - strand, within CA265_RS20255 at 5004.746 kb on + strand, within CA265_RS20255 at 5004.839 kb on - strand, within CA265_RS20255 at 5004.871 kb on - strand at 5004.894 kb on + strand at 5004.895 kb on - strand at 5004.943 kb on - strand at 5004.943 kb on - strand at 5004.987 kb on + strand at 5004.987 kb on + strand at 5005.014 kb on - strand at 5005.055 kb on - strand, within CA265_RS20260 at 5005.094 kb on - strand at 5005.213 kb on + strand, within CA265_RS20265 at 5005.213 kb on + strand, within CA265_RS20265 at 5005.254 kb on + strand, within CA265_RS20265 at 5005.317 kb on + strand at 5005.318 kb on - strand at 5005.322 kb on - strand at 5005.338 kb on - strand at 5005.338 kb on - strand at 5005.341 kb on + strand at 5005.341 kb on + strand at 5005.521 kb on - strand at 5005.521 kb on - strand at 5005.532 kb on - strand at 5005.541 kb on + strand at 5005.572 kb on - strand at 5005.583 kb on + strand at 5005.605 kb on + strand at 5005.648 kb on + strand at 5005.648 kb on + strand at 5005.662 kb on + strand at 5005.703 kb on - strand at 5005.892 kb on - strand, within CA265_RS20270 at 5005.940 kb on - strand, within CA265_RS20270 at 5005.942 kb on - strand, within CA265_RS20270 at 5005.942 kb on - strand, within CA265_RS20270 at 5005.960 kb on - strand, within CA265_RS20270 at 5007.823 kb on + strand at 5007.853 kb on + strand at 5007.909 kb on + strand, within CA265_RS20275 at 5007.958 kb on - strand, within CA265_RS20275 at 5007.960 kb on - strand, within CA265_RS20275 at 5008.037 kb on - strand, within CA265_RS20275 at 5008.049 kb on - strand, within CA265_RS20275 at 5008.084 kb on + strand, within CA265_RS20275 at 5008.085 kb on - strand, within CA265_RS20275 at 5008.171 kb on - strand, within CA265_RS20275 at 5008.171 kb on - strand, within CA265_RS20275 at 5008.198 kb on - strand, within CA265_RS20275 at 5008.287 kb on - strand, within CA265_RS20275 at 5008.287 kb on - strand, within CA265_RS20275 at 5008.287 kb on - strand, within CA265_RS20275 at 5008.349 kb on - strand, within CA265_RS20275 at 5008.443 kb on - strand, within CA265_RS20275 at 5008.449 kb on + strand, within CA265_RS20275 at 5008.449 kb on + strand, within CA265_RS20275 at 5008.652 kb on - strand at 5008.684 kb on - strand at 5008.713 kb on + strand at 5008.754 kb on + strand at 5008.771 kb on + strand, within CA265_RS20280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 5,004,673 - CA265_RS20255 0.69 +0.9 5,004,746 + CA265_RS20255 0.78 +0.2 5,004,839 - CA265_RS20255 0.89 -0.3 5,004,871 - -0.3 5,004,894 + -1.2 5,004,895 - -0.3 5,004,943 - +0.9 5,004,943 - +2.4 5,004,987 + -0.2 5,004,987 + +0.6 5,005,014 - -0.8 5,005,055 - CA265_RS20260 0.39 -0.9 5,005,094 - -1.1 5,005,213 + CA265_RS20265 0.49 -2.0 5,005,213 + CA265_RS20265 0.49 -0.9 5,005,254 + CA265_RS20265 0.66 -1.2 5,005,317 + -0.4 5,005,318 - +0.3 5,005,322 - -1.1 5,005,338 - +0.0 5,005,338 - -1.5 5,005,341 + -0.1 5,005,341 + +0.2 5,005,521 - +1.1 5,005,521 - +0.1 5,005,532 - -0.3 5,005,541 + -0.8 5,005,572 - -0.9 5,005,583 + +0.1 5,005,605 + -1.0 5,005,648 + +0.2 5,005,648 + +0.1 5,005,662 + +0.3 5,005,703 - -2.0 5,005,892 - CA265_RS20270 0.11 -1.6 5,005,940 - CA265_RS20270 0.13 +1.5 5,005,942 - CA265_RS20270 0.13 -0.1 5,005,942 - CA265_RS20270 0.13 +1.6 5,005,960 - CA265_RS20270 0.14 +1.9 5,007,823 + -0.5 5,007,853 + -0.9 5,007,909 + CA265_RS20275 0.10 -1.0 5,007,958 - CA265_RS20275 0.16 +0.3 5,007,960 - CA265_RS20275 0.16 +0.2 5,008,037 - CA265_RS20275 0.24 -1.2 5,008,049 - CA265_RS20275 0.26 -0.5 5,008,084 + CA265_RS20275 0.30 -0.8 5,008,085 - CA265_RS20275 0.30 -0.7 5,008,171 - CA265_RS20275 0.39 -0.4 5,008,171 - CA265_RS20275 0.39 -0.5 5,008,198 - CA265_RS20275 0.42 +0.4 5,008,287 - CA265_RS20275 0.52 +0.9 5,008,287 - CA265_RS20275 0.52 +0.3 5,008,287 - CA265_RS20275 0.52 -0.2 5,008,349 - CA265_RS20275 0.59 +0.2 5,008,443 - CA265_RS20275 0.69 -0.3 5,008,449 + CA265_RS20275 0.70 -0.4 5,008,449 + CA265_RS20275 0.70 +0.5 5,008,652 - +0.3 5,008,684 - +0.5 5,008,713 + +0.1 5,008,754 + -0.7 5,008,771 + CA265_RS20280 0.13 +0.6
Or see this region's nucleotide sequence