Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00005 and CA265_RS00010 are separated by 133 nucleotides CA265_RS00010 and CA265_RS00015 are separated by 39 nucleotides CA265_RS00015 and CA265_RS00020 are separated by 85 nucleotides CA265_RS00020 and CA265_RS00025 are separated by 3 nucleotides
CA265_RS00005: CA265_RS00005 - chromosomal replication initiation protein DnaA, at 1 to 1,431
_RS00005
CA265_RS00010: CA265_RS00010 - DNA mismatch repair protein MutT, at 1,565 to 2,113
_RS00010
CA265_RS00015: CA265_RS00015 - hypothetical protein, at 2,153 to 2,542
_RS00015
CA265_RS00020: CA265_RS00020 - methanol dehydrogenase, at 2,628 to 3,401
_RS00020
CA265_RS00025: CA265_RS00025 - hypothetical protein, at 3,405 to 3,848
_RS00025
Position (kb)
2
3 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1.454 kb on + strand at 1.496 kb on - strand at 1.503 kb on + strand at 1.504 kb on - strand at 1.522 kb on - strand at 1.538 kb on - strand at 1.538 kb on - strand at 1.593 kb on + strand at 1.594 kb on - strand at 1.595 kb on + strand at 1.596 kb on - strand at 1.630 kb on + strand, within CA265_RS00010 at 1.631 kb on - strand, within CA265_RS00010 at 1.640 kb on - strand, within CA265_RS00010 at 1.652 kb on + strand, within CA265_RS00010 at 1.666 kb on + strand, within CA265_RS00010 at 1.668 kb on + strand, within CA265_RS00010 at 1.668 kb on + strand, within CA265_RS00010 at 1.668 kb on + strand, within CA265_RS00010 at 1.686 kb on - strand, within CA265_RS00010 at 1.700 kb on + strand, within CA265_RS00010 at 1.701 kb on - strand, within CA265_RS00010 at 1.723 kb on + strand, within CA265_RS00010 at 1.741 kb on - strand, within CA265_RS00010 at 1.792 kb on + strand, within CA265_RS00010 at 1.877 kb on + strand, within CA265_RS00010 at 1.877 kb on + strand, within CA265_RS00010 at 1.958 kb on + strand, within CA265_RS00010 at 1.991 kb on + strand, within CA265_RS00010 at 1.998 kb on + strand, within CA265_RS00010 at 1.998 kb on + strand, within CA265_RS00010 at 1.999 kb on - strand, within CA265_RS00010 at 2.018 kb on - strand, within CA265_RS00010 at 2.032 kb on + strand, within CA265_RS00010 at 2.081 kb on - strand at 2.119 kb on + strand at 2.147 kb on - strand at 2.163 kb on + strand at 2.164 kb on - strand at 2.164 kb on - strand at 2.205 kb on - strand, within CA265_RS00015 at 2.229 kb on + strand, within CA265_RS00015 at 2.233 kb on + strand, within CA265_RS00015 at 2.233 kb on + strand, within CA265_RS00015 at 2.234 kb on - strand, within CA265_RS00015 at 2.282 kb on + strand, within CA265_RS00015 at 2.282 kb on + strand, within CA265_RS00015 at 2.296 kb on - strand, within CA265_RS00015 at 2.299 kb on - strand, within CA265_RS00015 at 2.458 kb on + strand, within CA265_RS00015 at 2.482 kb on + strand, within CA265_RS00015 at 2.482 kb on + strand, within CA265_RS00015 at 2.538 kb on + strand at 2.539 kb on - strand at 2.594 kb on - strand at 2.601 kb on - strand at 2.605 kb on + strand at 2.605 kb on + strand at 2.803 kb on - strand, within CA265_RS00020 at 2.829 kb on - strand, within CA265_RS00020 at 2.834 kb on + strand, within CA265_RS00020 at 2.864 kb on + strand, within CA265_RS00020 at 2.865 kb on - strand, within CA265_RS00020 at 2.876 kb on + strand, within CA265_RS00020 at 3.008 kb on + strand, within CA265_RS00020 at 3.009 kb on - strand, within CA265_RS00020 at 3.041 kb on + strand, within CA265_RS00020 at 3.042 kb on - strand, within CA265_RS00020 at 3.117 kb on - strand, within CA265_RS00020 at 3.175 kb on + strand, within CA265_RS00020 at 3.185 kb on + strand, within CA265_RS00020 at 3.185 kb on + strand, within CA265_RS00020 at 3.260 kb on + strand, within CA265_RS00020 at 3.260 kb on + strand, within CA265_RS00020 at 3.291 kb on + strand, within CA265_RS00020 at 3.294 kb on + strand, within CA265_RS00020 at 3.294 kb on + strand, within CA265_RS00020 at 3.299 kb on + strand, within CA265_RS00020 at 3.364 kb on - strand at 3.406 kb on + strand at 3.407 kb on - strand at 3.417 kb on + strand at 3.418 kb on - strand at 3.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 1,454 + -0.6 1,496 - +0.1 1,503 + +0.6 1,504 - +0.0 1,522 - +0.7 1,538 - -0.0 1,538 - -0.7 1,593 + -0.5 1,594 - -0.3 1,595 + -0.5 1,596 - -0.9 1,630 + CA265_RS00010 0.12 -0.9 1,631 - CA265_RS00010 0.12 +0.4 1,640 - CA265_RS00010 0.14 +0.4 1,652 + CA265_RS00010 0.16 -0.4 1,666 + CA265_RS00010 0.18 -0.9 1,668 + CA265_RS00010 0.19 +0.2 1,668 + CA265_RS00010 0.19 -0.4 1,668 + CA265_RS00010 0.19 +0.1 1,686 - CA265_RS00010 0.22 -0.1 1,700 + CA265_RS00010 0.25 -0.4 1,701 - CA265_RS00010 0.25 +0.7 1,723 + CA265_RS00010 0.29 +2.9 1,741 - CA265_RS00010 0.32 -0.8 1,792 + CA265_RS00010 0.41 +0.1 1,877 + CA265_RS00010 0.57 -1.0 1,877 + CA265_RS00010 0.57 +0.7 1,958 + CA265_RS00010 0.72 -0.3 1,991 + CA265_RS00010 0.78 +0.1 1,998 + CA265_RS00010 0.79 +0.2 1,998 + CA265_RS00010 0.79 +0.0 1,999 - CA265_RS00010 0.79 -0.5 2,018 - CA265_RS00010 0.83 +0.3 2,032 + CA265_RS00010 0.85 -1.0 2,081 - +0.1 2,119 + +0.5 2,147 - -0.2 2,163 + +0.2 2,164 - -1.1 2,164 - -0.6 2,205 - CA265_RS00015 0.13 +0.5 2,229 + CA265_RS00015 0.19 +0.3 2,233 + CA265_RS00015 0.21 +1.0 2,233 + CA265_RS00015 0.21 -0.4 2,234 - CA265_RS00015 0.21 -0.5 2,282 + CA265_RS00015 0.33 +0.5 2,282 + CA265_RS00015 0.33 -0.5 2,296 - CA265_RS00015 0.37 -1.2 2,299 - CA265_RS00015 0.37 +0.6 2,458 + CA265_RS00015 0.78 -0.1 2,482 + CA265_RS00015 0.84 +0.5 2,482 + CA265_RS00015 0.84 +1.5 2,538 + +0.4 2,539 - -0.3 2,594 - -1.0 2,601 - -1.3 2,605 + -0.3 2,605 + +0.1 2,803 - CA265_RS00020 0.23 -1.7 2,829 - CA265_RS00020 0.26 -0.4 2,834 + CA265_RS00020 0.27 +0.2 2,864 + CA265_RS00020 0.30 -0.3 2,865 - CA265_RS00020 0.31 -0.7 2,876 + CA265_RS00020 0.32 -0.6 3,008 + CA265_RS00020 0.49 -1.0 3,009 - CA265_RS00020 0.49 -0.9 3,041 + CA265_RS00020 0.53 -0.4 3,042 - CA265_RS00020 0.53 -0.1 3,117 - CA265_RS00020 0.63 +0.9 3,175 + CA265_RS00020 0.71 +0.9 3,185 + CA265_RS00020 0.72 -2.1 3,185 + CA265_RS00020 0.72 -0.2 3,260 + CA265_RS00020 0.82 +0.8 3,260 + CA265_RS00020 0.82 +0.1 3,291 + CA265_RS00020 0.86 -0.3 3,294 + CA265_RS00020 0.86 -0.5 3,294 + CA265_RS00020 0.86 -0.4 3,299 + CA265_RS00020 0.87 +0.4 3,364 - +0.1 3,406 + +1.1 3,407 - -0.1 3,417 + +0.4 3,418 - +0.8 3,418 - +0.5
Or see this region's nucleotide sequence