Experiment: CDM1 without Glutathione
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17720 and BBR_RS21005 are separated by 681 nucleotides BBR_RS21005 and BBR_RS21010 are separated by 19 nucleotides BBR_RS21010 and BBR_RS17730 are separated by 91 nucleotides
BBR_RS17720: BBR_RS17720 - CRISPR-associated helicase/endonuclease Cas3, at 1,754,432 to 1,756,870
_RS17720
BBR_RS21005: BBR_RS21005 - hypothetical protein, at 1,757,552 to 1,757,773
_RS21005
BBR_RS21010: BBR_RS21010 - hypothetical protein, at 1,757,793 to 1,757,990
_RS21010
BBR_RS17730: BBR_RS17730 - ATPase AAA, at 1,758,082 to 1,758,867
_RS17730
Position (kb)
1757
1758 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1756.585 kb on - strand, within BBR_RS17720 at 1756.685 kb on + strand at 1756.793 kb on + strand at 1756.801 kb on - strand at 1756.875 kb on - strand at 1756.935 kb on - strand at 1756.935 kb on - strand at 1756.993 kb on + strand at 1757.080 kb on - strand at 1757.134 kb on - strand at 1757.141 kb on + strand at 1757.141 kb on + strand at 1757.146 kb on - strand at 1757.149 kb on - strand at 1757.155 kb on - strand at 1757.231 kb on - strand at 1757.232 kb on - strand at 1757.296 kb on + strand at 1757.327 kb on + strand at 1757.327 kb on + strand at 1757.327 kb on - strand at 1757.327 kb on - strand at 1757.332 kb on - strand at 1757.413 kb on + strand at 1757.426 kb on - strand at 1757.459 kb on + strand at 1757.465 kb on - strand at 1757.537 kb on + strand at 1757.550 kb on + strand at 1757.558 kb on - strand at 1757.608 kb on - strand, within BBR_RS21005 at 1757.615 kb on - strand, within BBR_RS21005 at 1757.678 kb on + strand, within BBR_RS21005 at 1757.678 kb on + strand, within BBR_RS21005 at 1757.688 kb on + strand, within BBR_RS21005 at 1757.688 kb on + strand, within BBR_RS21005 at 1757.688 kb on + strand, within BBR_RS21005 at 1757.693 kb on + strand, within BBR_RS21005 at 1757.696 kb on - strand, within BBR_RS21005 at 1757.698 kb on + strand, within BBR_RS21005 at 1757.706 kb on - strand, within BBR_RS21005 at 1757.712 kb on + strand, within BBR_RS21005 at 1757.729 kb on - strand, within BBR_RS21005 at 1757.748 kb on + strand, within BBR_RS21005 at 1757.767 kb on + strand at 1757.772 kb on + strand at 1757.772 kb on - strand at 1757.775 kb on - strand at 1757.783 kb on + strand at 1757.787 kb on - strand at 1757.796 kb on - strand at 1757.796 kb on - strand at 1757.832 kb on - strand, within BBR_RS21010 at 1757.834 kb on - strand, within BBR_RS21010 at 1757.905 kb on + strand, within BBR_RS21010 at 1757.931 kb on - strand, within BBR_RS21010 at 1757.969 kb on - strand, within BBR_RS21010 at 1757.969 kb on - strand, within BBR_RS21010
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Glutathione remove 1,756,585 - BBR_RS17720 0.88 -2.3 1,756,685 + -2.5 1,756,793 + +1.0 1,756,801 - +0.2 1,756,875 - -0.9 1,756,935 - -4.0 1,756,935 - -3.6 1,756,993 + +0.4 1,757,080 - -0.7 1,757,134 - -0.3 1,757,141 + +0.0 1,757,141 + +0.8 1,757,146 - +0.5 1,757,149 - +0.9 1,757,155 - +0.5 1,757,231 - -0.4 1,757,232 - -1.6 1,757,296 + -1.6 1,757,327 + -2.3 1,757,327 + -1.9 1,757,327 - -0.7 1,757,327 - +0.3 1,757,332 - -1.0 1,757,413 + -2.7 1,757,426 - -0.8 1,757,459 + +0.4 1,757,465 - -0.8 1,757,537 + +0.1 1,757,550 + -0.8 1,757,558 - -0.9 1,757,608 - BBR_RS21005 0.25 -1.3 1,757,615 - BBR_RS21005 0.28 -0.9 1,757,678 + BBR_RS21005 0.57 -0.3 1,757,678 + BBR_RS21005 0.57 +0.3 1,757,688 + BBR_RS21005 0.61 -2.1 1,757,688 + BBR_RS21005 0.61 -1.2 1,757,688 + BBR_RS21005 0.61 -0.8 1,757,693 + BBR_RS21005 0.64 +0.9 1,757,696 - BBR_RS21005 0.65 -0.6 1,757,698 + BBR_RS21005 0.66 -2.0 1,757,706 - BBR_RS21005 0.69 -0.8 1,757,712 + BBR_RS21005 0.72 -0.9 1,757,729 - BBR_RS21005 0.80 -0.7 1,757,748 + BBR_RS21005 0.88 -0.3 1,757,767 + +0.4 1,757,772 + +0.7 1,757,772 - -1.3 1,757,775 - -3.8 1,757,783 + +0.5 1,757,787 - -0.4 1,757,796 - -1.4 1,757,796 - +0.4 1,757,832 - BBR_RS21010 0.20 -0.7 1,757,834 - BBR_RS21010 0.21 -0.7 1,757,905 + BBR_RS21010 0.57 -2.9 1,757,931 - BBR_RS21010 0.70 -0.2 1,757,969 - BBR_RS21010 0.89 -1.3 1,757,969 - BBR_RS21010 0.89 -0.2
Or see this region's nucleotide sequence