Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3706 and Shewana3_3707 are separated by 31 nucleotides Shewana3_3707 and Shewana3_3708 overlap by 4 nucleotides Shewana3_3708 and Shewana3_3709 overlap by 8 nucleotides
Shewana3_3706: Shewana3_3706 - hypothetical protein (RefSeq), at 4,448,280 to 4,448,756
_3706
Shewana3_3707: Shewana3_3707 - response regulator receiver protein (RefSeq), at 4,448,788 to 4,449,339
_3707
Shewana3_3708: Shewana3_3708 - ATPase domain-containing protein (RefSeq), at 4,449,336 to 4,450,625
_3708
Shewana3_3709: Shewana3_3709 - dTDP-4-dehydrorhamnose reductase (RefSeq), at 4,450,618 to 4,451,547
_3709
Position (kb) 4449
4450
4451 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4448.567 kb on - strand, within Shewana3_3706 at 4448.732 kb on + strand at 4448.758 kb on + strand at 4448.934 kb on + strand, within Shewana3_3707 at 4448.942 kb on - strand, within Shewana3_3707 at 4448.942 kb on - strand, within Shewana3_3707 at 4449.212 kb on - strand, within Shewana3_3707 at 4449.429 kb on + strand at 4449.491 kb on - strand, within Shewana3_3708 at 4449.491 kb on - strand, within Shewana3_3708 at 4449.516 kb on + strand, within Shewana3_3708 at 4449.617 kb on - strand, within Shewana3_3708 at 4449.678 kb on + strand, within Shewana3_3708 at 4449.715 kb on + strand, within Shewana3_3708 at 4449.753 kb on - strand, within Shewana3_3708 at 4449.891 kb on + strand, within Shewana3_3708 at 4449.899 kb on - strand, within Shewana3_3708 at 4450.055 kb on - strand, within Shewana3_3708 at 4450.112 kb on + strand, within Shewana3_3708 at 4450.125 kb on - strand, within Shewana3_3708 at 4450.232 kb on + strand, within Shewana3_3708 at 4450.240 kb on - strand, within Shewana3_3708 at 4450.394 kb on + strand, within Shewana3_3708 at 4450.404 kb on - strand, within Shewana3_3708 at 4450.429 kb on + strand, within Shewana3_3708 at 4450.519 kb on - strand at 4450.567 kb on + strand at 4450.567 kb on + strand at 4450.567 kb on - strand at 4450.575 kb on - strand at 4450.788 kb on - strand, within Shewana3_3709 at 4450.797 kb on + strand, within Shewana3_3709 at 4450.822 kb on - strand, within Shewana3_3709 at 4450.833 kb on + strand, within Shewana3_3709 at 4451.118 kb on + strand, within Shewana3_3709 at 4451.121 kb on - strand, within Shewana3_3709 at 4451.126 kb on - strand, within Shewana3_3709 at 4451.174 kb on - strand, within Shewana3_3709 at 4451.199 kb on - strand, within Shewana3_3709 at 4451.303 kb on - strand, within Shewana3_3709 at 4451.328 kb on + strand, within Shewana3_3709 at 4451.328 kb on + strand, within Shewana3_3709 at 4451.381 kb on - strand, within Shewana3_3709 at 4451.623 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,448,567 - Shewana3_3706 0.60 +0.6 4,448,732 + +0.3 4,448,758 + +0.8 4,448,934 + Shewana3_3707 0.26 -0.3 4,448,942 - Shewana3_3707 0.28 -1.4 4,448,942 - Shewana3_3707 0.28 +0.3 4,449,212 - Shewana3_3707 0.77 -3.2 4,449,429 + +1.1 4,449,491 - Shewana3_3708 0.12 +0.3 4,449,491 - Shewana3_3708 0.12 -1.6 4,449,516 + Shewana3_3708 0.14 -2.4 4,449,617 - Shewana3_3708 0.22 -0.1 4,449,678 + Shewana3_3708 0.27 +0.8 4,449,715 + Shewana3_3708 0.29 +0.7 4,449,753 - Shewana3_3708 0.32 -1.5 4,449,891 + Shewana3_3708 0.43 -3.3 4,449,899 - Shewana3_3708 0.44 -2.0 4,450,055 - Shewana3_3708 0.56 -1.5 4,450,112 + Shewana3_3708 0.60 +0.1 4,450,125 - Shewana3_3708 0.61 -1.9 4,450,232 + Shewana3_3708 0.69 -0.3 4,450,240 - Shewana3_3708 0.70 +0.3 4,450,394 + Shewana3_3708 0.82 +0.1 4,450,404 - Shewana3_3708 0.83 +0.5 4,450,429 + Shewana3_3708 0.85 -4.0 4,450,519 - +0.8 4,450,567 + -0.9 4,450,567 + +0.3 4,450,567 - +1.4 4,450,575 - +0.5 4,450,788 - Shewana3_3709 0.18 +0.6 4,450,797 + Shewana3_3709 0.19 -1.6 4,450,822 - Shewana3_3709 0.22 -1.9 4,450,833 + Shewana3_3709 0.23 -1.3 4,451,118 + Shewana3_3709 0.54 +0.9 4,451,121 - Shewana3_3709 0.54 +0.0 4,451,126 - Shewana3_3709 0.55 +1.4 4,451,174 - Shewana3_3709 0.60 +0.2 4,451,199 - Shewana3_3709 0.62 -0.4 4,451,303 - Shewana3_3709 0.74 -0.1 4,451,328 + Shewana3_3709 0.76 +0.3 4,451,328 + Shewana3_3709 0.76 +0.2 4,451,381 - Shewana3_3709 0.82 -0.2 4,451,623 - -1.3
Or see this region's nucleotide sequence