Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3605 and Shewana3_3606 are separated by 835 nucleotides Shewana3_3606 and Shewana3_3607 are separated by 10 nucleotides Shewana3_3607 and Shewana3_3608 are separated by 189 nucleotides
Shewana3_3605: Shewana3_3605 - hypothetical protein (RefSeq), at 4,329,321 to 4,329,695
_3605
Shewana3_3606: Shewana3_3606 - cytochrome c family protein (RefSeq), at 4,330,531 to 4,331,589
_3606
Shewana3_3607: Shewana3_3607 - cytochrome c family protein (RefSeq), at 4,331,600 to 4,332,226
_3607
Shewana3_3608: Shewana3_3608 - response regulator receiver protein (RefSeq), at 4,332,416 to 4,333,444
_3608
Position (kb) 4330
4331
4332 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4329.533 kb on + strand, within Shewana3_3605 at 4329.563 kb on + strand, within Shewana3_3605 at 4329.563 kb on + strand, within Shewana3_3605 at 4329.563 kb on + strand, within Shewana3_3605 at 4329.563 kb on + strand, within Shewana3_3605 at 4329.572 kb on - strand, within Shewana3_3605 at 4329.572 kb on - strand, within Shewana3_3605 at 4329.681 kb on - strand at 4329.700 kb on - strand at 4329.702 kb on + strand at 4329.709 kb on + strand at 4329.710 kb on - strand at 4329.710 kb on - strand at 4329.710 kb on - strand at 4329.717 kb on - strand at 4329.758 kb on + strand at 4329.758 kb on + strand at 4329.766 kb on - strand at 4329.766 kb on - strand at 4330.013 kb on + strand at 4330.013 kb on + strand at 4330.014 kb on - strand at 4330.021 kb on - strand at 4330.022 kb on + strand at 4330.606 kb on - strand at 4330.657 kb on - strand, within Shewana3_3606 at 4330.657 kb on - strand, within Shewana3_3606 at 4330.733 kb on - strand, within Shewana3_3606 at 4330.783 kb on - strand, within Shewana3_3606 at 4330.786 kb on + strand, within Shewana3_3606 at 4330.803 kb on + strand, within Shewana3_3606 at 4330.811 kb on - strand, within Shewana3_3606 at 4330.812 kb on - strand, within Shewana3_3606 at 4330.861 kb on + strand, within Shewana3_3606 at 4330.861 kb on + strand, within Shewana3_3606 at 4330.869 kb on - strand, within Shewana3_3606 at 4330.894 kb on - strand, within Shewana3_3606 at 4330.903 kb on + strand, within Shewana3_3606 at 4330.918 kb on - strand, within Shewana3_3606 at 4330.997 kb on - strand, within Shewana3_3606 at 4331.011 kb on - strand, within Shewana3_3606 at 4331.056 kb on - strand, within Shewana3_3606 at 4331.087 kb on + strand, within Shewana3_3606 at 4331.092 kb on - strand, within Shewana3_3606 at 4331.129 kb on - strand, within Shewana3_3606 at 4331.129 kb on - strand, within Shewana3_3606 at 4331.129 kb on - strand, within Shewana3_3606 at 4331.164 kb on - strand, within Shewana3_3606 at 4331.165 kb on - strand, within Shewana3_3606 at 4331.165 kb on - strand, within Shewana3_3606 at 4331.211 kb on + strand, within Shewana3_3606 at 4331.219 kb on - strand, within Shewana3_3606 at 4331.338 kb on + strand, within Shewana3_3606 at 4331.346 kb on - strand, within Shewana3_3606 at 4331.492 kb on - strand at 4331.689 kb on - strand, within Shewana3_3607 at 4331.818 kb on - strand, within Shewana3_3607 at 4331.828 kb on - strand, within Shewana3_3607 at 4331.907 kb on + strand, within Shewana3_3607 at 4332.035 kb on + strand, within Shewana3_3607 at 4332.164 kb on + strand at 4332.164 kb on + strand at 4332.172 kb on - strand at 4332.172 kb on - strand at 4332.291 kb on - strand at 4332.381 kb on - strand at 4332.455 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,329,533 + Shewana3_3605 0.57 +0.6 4,329,563 + Shewana3_3605 0.65 -1.7 4,329,563 + Shewana3_3605 0.65 +0.3 4,329,563 + Shewana3_3605 0.65 -1.9 4,329,563 + Shewana3_3605 0.65 -2.4 4,329,572 - Shewana3_3605 0.67 +0.5 4,329,572 - Shewana3_3605 0.67 +0.9 4,329,681 - -0.5 4,329,700 - +0.3 4,329,702 + -0.1 4,329,709 + +0.7 4,329,710 - -1.1 4,329,710 - -1.2 4,329,710 - -1.6 4,329,717 - +0.4 4,329,758 + -1.4 4,329,758 + +1.0 4,329,766 - +0.5 4,329,766 - -1.4 4,330,013 + +0.6 4,330,013 + -1.7 4,330,014 - +0.4 4,330,021 - -1.0 4,330,022 + -0.5 4,330,606 - +1.0 4,330,657 - Shewana3_3606 0.12 +0.5 4,330,657 - Shewana3_3606 0.12 +0.9 4,330,733 - Shewana3_3606 0.19 +0.4 4,330,783 - Shewana3_3606 0.24 +0.8 4,330,786 + Shewana3_3606 0.24 +0.2 4,330,803 + Shewana3_3606 0.26 +1.8 4,330,811 - Shewana3_3606 0.26 +1.4 4,330,812 - Shewana3_3606 0.27 +0.1 4,330,861 + Shewana3_3606 0.31 -1.4 4,330,861 + Shewana3_3606 0.31 +0.7 4,330,869 - Shewana3_3606 0.32 -0.3 4,330,894 - Shewana3_3606 0.34 -0.4 4,330,903 + Shewana3_3606 0.35 -0.6 4,330,918 - Shewana3_3606 0.37 +0.6 4,330,997 - Shewana3_3606 0.44 -2.3 4,331,011 - Shewana3_3606 0.45 +0.4 4,331,056 - Shewana3_3606 0.50 -1.9 4,331,087 + Shewana3_3606 0.53 -1.4 4,331,092 - Shewana3_3606 0.53 +0.9 4,331,129 - Shewana3_3606 0.56 -0.1 4,331,129 - Shewana3_3606 0.56 +0.5 4,331,129 - Shewana3_3606 0.56 +0.1 4,331,164 - Shewana3_3606 0.60 -0.3 4,331,165 - Shewana3_3606 0.60 +0.3 4,331,165 - Shewana3_3606 0.60 +0.3 4,331,211 + Shewana3_3606 0.64 -2.2 4,331,219 - Shewana3_3606 0.65 +0.4 4,331,338 + Shewana3_3606 0.76 +0.6 4,331,346 - Shewana3_3606 0.77 +0.4 4,331,492 - +0.4 4,331,689 - Shewana3_3607 0.14 +0.8 4,331,818 - Shewana3_3607 0.35 -2.2 4,331,828 - Shewana3_3607 0.36 -2.4 4,331,907 + Shewana3_3607 0.49 -0.9 4,332,035 + Shewana3_3607 0.69 +0.3 4,332,164 + +0.2 4,332,164 + -2.5 4,332,172 - -2.8 4,332,172 - +1.3 4,332,291 - +0.4 4,332,381 - -0.0 4,332,455 + -2.0
Or see this region's nucleotide sequence