Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3225 and Shewana3_3226 are separated by 203 nucleotides Shewana3_3226 and Shewana3_3227 are separated by 511 nucleotides
Shewana3_3225: Shewana3_3225 - peptidase S10, serine carboxypeptidase (RefSeq), at 3,866,794 to 3,868,344
_3225
Shewana3_3226: Shewana3_3226 - thiol:disulfide interchange protein, putative (RefSeq), at 3,868,548 to 3,869,093
_3226
Shewana3_3227: Shewana3_3227 - Fis family transcriptional regulator (RefSeq), at 3,869,605 to 3,871,071
_3227
Position (kb)
3868
3869
3870 Strain fitness (log2 ratio)
-2
-1
0
1 at 3867.826 kb on - strand, within Shewana3_3225 at 3867.841 kb on + strand, within Shewana3_3225 at 3867.841 kb on + strand, within Shewana3_3225 at 3868.009 kb on + strand, within Shewana3_3225 at 3868.054 kb on + strand, within Shewana3_3225 at 3868.211 kb on + strand at 3868.267 kb on - strand at 3868.366 kb on - strand at 3868.563 kb on - strand at 3868.640 kb on - strand, within Shewana3_3226 at 3868.640 kb on - strand, within Shewana3_3226 at 3868.717 kb on - strand, within Shewana3_3226 at 3868.986 kb on - strand, within Shewana3_3226 at 3869.005 kb on - strand, within Shewana3_3226 at 3869.031 kb on - strand, within Shewana3_3226 at 3869.043 kb on - strand at 3869.254 kb on - strand at 3869.349 kb on - strand at 3869.495 kb on - strand at 3869.523 kb on + strand at 3869.617 kb on - strand at 3869.668 kb on + strand at 3869.678 kb on - strand at 3869.690 kb on + strand at 3869.712 kb on + strand at 3869.943 kb on - strand, within Shewana3_3227 at 3870.051 kb on + strand, within Shewana3_3227 at 3870.059 kb on - strand, within Shewana3_3227
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,867,826 - Shewana3_3225 0.67 +0.2 3,867,841 + Shewana3_3225 0.68 +0.7 3,867,841 + Shewana3_3225 0.68 -0.7 3,868,009 + Shewana3_3225 0.78 +0.1 3,868,054 + Shewana3_3225 0.81 -0.9 3,868,211 + -1.5 3,868,267 - -1.7 3,868,366 - -1.5 3,868,563 - +0.2 3,868,640 - Shewana3_3226 0.17 +0.9 3,868,640 - Shewana3_3226 0.17 -1.2 3,868,717 - Shewana3_3226 0.31 +0.6 3,868,986 - Shewana3_3226 0.80 -0.5 3,869,005 - Shewana3_3226 0.84 +1.3 3,869,031 - Shewana3_3226 0.88 -1.7 3,869,043 - -2.0 3,869,254 - +0.3 3,869,349 - +0.2 3,869,495 - +0.6 3,869,523 + -0.3 3,869,617 - +0.4 3,869,668 + +0.2 3,869,678 - +0.5 3,869,690 + +0.3 3,869,712 + +0.4 3,869,943 - Shewana3_3227 0.23 -0.4 3,870,051 + Shewana3_3227 0.30 +0.1 3,870,059 - Shewana3_3227 0.31 -0.0
Or see this region's nucleotide sequence