Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgi and Shewana3_3149 are separated by 216 nucleotides Shewana3_3149 and Shewana3_3150 are separated by 67 nucleotides Shewana3_3150 and Shewana3_3151 are separated by 222 nucleotides Shewana3_3151 and Shewana3_3152 overlap by 8 nucleotides
Shewana3_3148: pgi - glucose-6-phosphate isomerase (RefSeq), at 3,776,328 to 3,777,965
pgi
Shewana3_3149: Shewana3_3149 - hypothetical protein (RefSeq), at 3,778,182 to 3,778,367
_3149
Shewana3_3150: Shewana3_3150 - hemerythrin HHE cation binding domain-containing protein (RefSeq), at 3,778,435 to 3,778,971
_3150
Shewana3_3151: Shewana3_3151 - hypothetical protein (RefSeq), at 3,779,194 to 3,779,901
_3151
Shewana3_3152: Shewana3_3152 - ECF subfamily RNA polymerase sigma-24 factor (RefSeq), at 3,779,894 to 3,780,496
_3152
Position (kb) 3778
3779 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3778.218 kb on + strand, within Shewana3_3149 at 3778.448 kb on - strand at 3778.453 kb on - strand at 3778.727 kb on - strand, within Shewana3_3150 at 3778.851 kb on - strand, within Shewana3_3150 at 3779.411 kb on - strand, within Shewana3_3151 at 3779.884 kb on - strand at 3779.950 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,778,218 + Shewana3_3149 0.19 +0.6 3,778,448 - -0.5 3,778,453 - -0.2 3,778,727 - Shewana3_3150 0.54 +0.5 3,778,851 - Shewana3_3150 0.77 +0.5 3,779,411 - Shewana3_3151 0.31 -2.8 3,779,884 - -0.2 3,779,950 - +0.5
Or see this region's nucleotide sequence