Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3073 and Shewana3_3074 are separated by 63 nucleotides Shewana3_3074 and Shewana3_3075 overlap by 1 nucleotides
Shewana3_3073: Shewana3_3073 - citrate transporter (RefSeq), at 3,685,305 to 3,686,615
_3073
Shewana3_3074: Shewana3_3074 - general secretion pathway protein B (RefSeq), at 3,686,679 to 3,687,779
_3074
Shewana3_3075: Shewana3_3075 - peptidoglycan binding domain-containing protein (RefSeq), at 3,687,779 to 3,689,467
_3075
Position (kb)
3686
3687
3688 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3686.223 kb on + strand, within Shewana3_3073 at 3686.347 kb on + strand, within Shewana3_3073 at 3686.387 kb on + strand, within Shewana3_3073 at 3686.395 kb on - strand, within Shewana3_3073 at 3686.395 kb on - strand, within Shewana3_3073 at 3687.099 kb on - strand, within Shewana3_3074 at 3687.187 kb on - strand, within Shewana3_3074 at 3687.316 kb on + strand, within Shewana3_3074 at 3687.612 kb on - strand, within Shewana3_3074 at 3687.734 kb on - strand at 3687.811 kb on + strand at 3687.867 kb on + strand at 3688.010 kb on - strand, within Shewana3_3075 at 3688.291 kb on + strand, within Shewana3_3075 at 3688.292 kb on + strand, within Shewana3_3075 at 3688.324 kb on + strand, within Shewana3_3075 at 3688.492 kb on - strand, within Shewana3_3075 at 3688.684 kb on + strand, within Shewana3_3075
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,686,223 + Shewana3_3073 0.70 -2.0 3,686,347 + Shewana3_3073 0.79 -0.3 3,686,387 + Shewana3_3073 0.83 -3.0 3,686,395 - Shewana3_3073 0.83 +0.6 3,686,395 - Shewana3_3073 0.83 +0.0 3,687,099 - Shewana3_3074 0.38 +0.8 3,687,187 - Shewana3_3074 0.46 +0.8 3,687,316 + Shewana3_3074 0.58 -2.4 3,687,612 - Shewana3_3074 0.85 +0.8 3,687,734 - +0.7 3,687,811 + -1.2 3,687,867 + +0.8 3,688,010 - Shewana3_3075 0.14 +0.9 3,688,291 + Shewana3_3075 0.30 +1.8 3,688,292 + Shewana3_3075 0.30 -0.3 3,688,324 + Shewana3_3075 0.32 +1.4 3,688,492 - Shewana3_3075 0.42 +0.0 3,688,684 + Shewana3_3075 0.54 +1.5
Or see this region's nucleotide sequence