Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3053 and Shewana3_3054 are separated by 55 nucleotides Shewana3_3054 and Shewana3_3055 overlap by 4 nucleotides Shewana3_3055 and Shewana3_3056 are separated by 1 nucleotides Shewana3_3056 and Shewana3_3057 are separated by 122 nucleotides
Shewana3_3053: Shewana3_3053 - cobalamin biosynthesis protein CbiB (RefSeq), at 3,661,068 to 3,662,057
_3053
Shewana3_3054: Shewana3_3054 - hypothetical protein (RefSeq), at 3,662,113 to 3,662,490
_3054
Shewana3_3055: Shewana3_3055 - hypothetical protein (RefSeq), at 3,662,487 to 3,663,113
_3055
Shewana3_3056: Shewana3_3056 - hypothetical protein (RefSeq), at 3,663,115 to 3,663,606
_3056
Shewana3_3057: Shewana3_3057 - hypothetical protein (RefSeq), at 3,663,729 to 3,664,157
_3057
Position (kb)
3662
3663
3664 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3661.611 kb on - strand, within Shewana3_3053 at 3661.878 kb on + strand, within Shewana3_3053 at 3661.908 kb on - strand, within Shewana3_3053 at 3661.918 kb on - strand, within Shewana3_3053 at 3662.042 kb on + strand at 3662.211 kb on - strand, within Shewana3_3054 at 3662.220 kb on + strand, within Shewana3_3054 at 3662.313 kb on - strand, within Shewana3_3054 at 3662.315 kb on + strand, within Shewana3_3054 at 3662.714 kb on - strand, within Shewana3_3055 at 3662.770 kb on - strand, within Shewana3_3055 at 3662.872 kb on - strand, within Shewana3_3055 at 3662.889 kb on + strand, within Shewana3_3055 at 3662.922 kb on + strand, within Shewana3_3055 at 3663.140 kb on + strand at 3663.188 kb on - strand, within Shewana3_3056 at 3663.254 kb on + strand, within Shewana3_3056 at 3663.444 kb on + strand, within Shewana3_3056 at 3663.500 kb on + strand, within Shewana3_3056 at 3663.515 kb on + strand, within Shewana3_3056 at 3663.589 kb on - strand at 3663.769 kb on + strand at 3663.931 kb on + strand, within Shewana3_3057 at 3664.052 kb on - strand, within Shewana3_3057
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,661,611 - Shewana3_3053 0.55 -1.5 3,661,878 + Shewana3_3053 0.82 -2.5 3,661,908 - Shewana3_3053 0.85 -3.3 3,661,918 - Shewana3_3053 0.86 -2.1 3,662,042 + -1.5 3,662,211 - Shewana3_3054 0.26 +0.8 3,662,220 + Shewana3_3054 0.28 +0.5 3,662,313 - Shewana3_3054 0.53 -0.9 3,662,315 + Shewana3_3054 0.53 -2.0 3,662,714 - Shewana3_3055 0.36 +0.2 3,662,770 - Shewana3_3055 0.45 +0.3 3,662,872 - Shewana3_3055 0.61 -1.7 3,662,889 + Shewana3_3055 0.64 +0.4 3,662,922 + Shewana3_3055 0.69 +0.1 3,663,140 + +0.4 3,663,188 - Shewana3_3056 0.15 -2.0 3,663,254 + Shewana3_3056 0.28 -1.2 3,663,444 + Shewana3_3056 0.67 +0.8 3,663,500 + Shewana3_3056 0.78 -0.1 3,663,515 + Shewana3_3056 0.81 -1.3 3,663,589 - +0.3 3,663,769 + -1.1 3,663,931 + Shewana3_3057 0.47 -0.0 3,664,052 - Shewana3_3057 0.75 +0.9
Or see this region's nucleotide sequence