Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3043 and Shewana3_3044 are separated by 691 nucleotides Shewana3_3044 and Shewana3_3045 are separated by 183 nucleotides
Shewana3_3043: Shewana3_3043 - dinucleoside polyphosphate hydrolase (RefSeq), at 3,647,913 to 3,648,437
_3043
Shewana3_3044: Shewana3_3044 - DNA mismatch repair protein (RefSeq), at 3,649,129 to 3,649,800
_3044
Shewana3_3045: Shewana3_3045 - adenylate cyclase-related protein (RefSeq), at 3,649,984 to 3,650,991
_3045
Position (kb)
3649
3650 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3648.332 kb on - strand, within Shewana3_3043 at 3648.367 kb on - strand, within Shewana3_3043 at 3648.441 kb on + strand at 3648.818 kb on - strand at 3649.051 kb on + strand at 3649.059 kb on - strand at 3649.064 kb on - strand at 3649.167 kb on + strand at 3649.219 kb on + strand, within Shewana3_3044 at 3649.244 kb on - strand, within Shewana3_3044 at 3649.348 kb on - strand, within Shewana3_3044 at 3649.471 kb on + strand, within Shewana3_3044 at 3649.529 kb on - strand, within Shewana3_3044 at 3649.572 kb on - strand, within Shewana3_3044 at 3649.657 kb on - strand, within Shewana3_3044 at 3649.676 kb on - strand, within Shewana3_3044 at 3649.982 kb on - strand at 3650.223 kb on + strand, within Shewana3_3045 at 3650.344 kb on + strand, within Shewana3_3045 at 3650.345 kb on + strand, within Shewana3_3045 at 3650.441 kb on + strand, within Shewana3_3045 at 3650.761 kb on + strand, within Shewana3_3045
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,648,332 - Shewana3_3043 0.80 -1.4 3,648,367 - Shewana3_3043 0.86 -3.2 3,648,441 + +0.3 3,648,818 - +0.0 3,649,051 + +0.6 3,649,059 - -2.1 3,649,064 - -0.3 3,649,167 + -1.1 3,649,219 + Shewana3_3044 0.13 -0.7 3,649,244 - Shewana3_3044 0.17 -0.0 3,649,348 - Shewana3_3044 0.33 +0.1 3,649,471 + Shewana3_3044 0.51 -2.2 3,649,529 - Shewana3_3044 0.60 -2.2 3,649,572 - Shewana3_3044 0.66 +0.0 3,649,657 - Shewana3_3044 0.79 -1.4 3,649,676 - Shewana3_3044 0.81 +0.3 3,649,982 - +0.9 3,650,223 + Shewana3_3045 0.24 -0.8 3,650,344 + Shewana3_3045 0.36 +0.6 3,650,345 + Shewana3_3045 0.36 -0.6 3,650,441 + Shewana3_3045 0.45 +0.4 3,650,761 + Shewana3_3045 0.77 -1.1
Or see this region's nucleotide sequence