Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2996 and Shewana3_2997 are separated by 33 nucleotides Shewana3_2997 and Shewana3_2998 are separated by 122 nucleotides Shewana3_2998 and Shewana3_2999 are separated by 69 nucleotides
Shewana3_2996: Shewana3_2996 - band 7 protein (RefSeq), at 3,601,129 to 3,602,907
_2996
Shewana3_2997: Shewana3_2997 - hypothetical protein (RefSeq), at 3,602,941 to 3,603,576
_2997
Shewana3_2998: Shewana3_2998 - hypothetical protein (RefSeq), at 3,603,699 to 3,604,265
_2998
Shewana3_2999: Shewana3_2999 - peptidase M32, carboxypeptidase Taq metallopeptidase (RefSeq), at 3,604,335 to 3,605,840
_2999
Position (kb)
3603
3604
3605 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3602.763 kb on - strand at 3602.825 kb on + strand at 3603.084 kb on - strand, within Shewana3_2997 at 3603.092 kb on + strand, within Shewana3_2997 at 3603.471 kb on - strand, within Shewana3_2997 at 3603.477 kb on - strand, within Shewana3_2997 at 3603.495 kb on - strand, within Shewana3_2997 at 3603.523 kb on + strand at 3603.645 kb on - strand at 3603.892 kb on + strand, within Shewana3_2998 at 3603.945 kb on + strand, within Shewana3_2998 at 3604.068 kb on - strand, within Shewana3_2998 at 3604.090 kb on - strand, within Shewana3_2998 at 3604.114 kb on - strand, within Shewana3_2998 at 3604.117 kb on + strand, within Shewana3_2998 at 3604.229 kb on - strand at 3604.523 kb on + strand, within Shewana3_2999 at 3604.590 kb on - strand, within Shewana3_2999 at 3604.590 kb on - strand, within Shewana3_2999 at 3604.787 kb on - strand, within Shewana3_2999 at 3604.816 kb on + strand, within Shewana3_2999 at 3605.011 kb on + strand, within Shewana3_2999 at 3605.083 kb on - strand, within Shewana3_2999 at 3605.169 kb on + strand, within Shewana3_2999
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,602,763 - -1.3 3,602,825 + -1.4 3,603,084 - Shewana3_2997 0.22 -0.1 3,603,092 + Shewana3_2997 0.24 -0.1 3,603,471 - Shewana3_2997 0.83 -0.5 3,603,477 - Shewana3_2997 0.84 -1.1 3,603,495 - Shewana3_2997 0.87 -0.8 3,603,523 + -0.3 3,603,645 - +0.3 3,603,892 + Shewana3_2998 0.34 -0.0 3,603,945 + Shewana3_2998 0.43 -0.5 3,604,068 - Shewana3_2998 0.65 +0.5 3,604,090 - Shewana3_2998 0.69 +0.2 3,604,114 - Shewana3_2998 0.73 +2.0 3,604,117 + Shewana3_2998 0.74 +1.0 3,604,229 - -0.7 3,604,523 + Shewana3_2999 0.12 +0.3 3,604,590 - Shewana3_2999 0.17 +1.2 3,604,590 - Shewana3_2999 0.17 -1.4 3,604,787 - Shewana3_2999 0.30 +0.6 3,604,816 + Shewana3_2999 0.32 +0.7 3,605,011 + Shewana3_2999 0.45 +0.5 3,605,083 - Shewana3_2999 0.50 -4.8 3,605,169 + Shewana3_2999 0.55 -1.0
Or see this region's nucleotide sequence