Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2961 and Shewana3_2962 are separated by 26 nucleotides Shewana3_2962 and hsdR are separated by 125 nucleotides
Shewana3_2961: Shewana3_2961 - N-6 DNA methylase (RefSeq), at 3,555,768 to 3,557,360
_2961
Shewana3_2962: Shewana3_2962 - filamentation induced by cAMP protein Fic (RefSeq), at 3,557,387 to 3,558,514
_2962
Shewana3_2963: hsdR - type I restriction enzyme EcoKI subunit R (RefSeq), at 3,558,640 to 3,562,143
hsdR
Position (kb)
3557
3558
3559 Strain fitness (log2 ratio)
-2
-1
0
1 at 3556.466 kb on - strand, within Shewana3_2961 at 3556.545 kb on + strand, within Shewana3_2961 at 3556.572 kb on + strand, within Shewana3_2961 at 3556.666 kb on - strand, within Shewana3_2961 at 3556.858 kb on - strand, within Shewana3_2961 at 3556.865 kb on - strand, within Shewana3_2961 at 3556.865 kb on - strand, within Shewana3_2961 at 3556.944 kb on + strand, within Shewana3_2961 at 3556.947 kb on - strand, within Shewana3_2961 at 3557.027 kb on - strand, within Shewana3_2961 at 3557.034 kb on + strand, within Shewana3_2961 at 3557.042 kb on - strand, within Shewana3_2961 at 3557.100 kb on - strand, within Shewana3_2961 at 3557.454 kb on - strand at 3557.550 kb on + strand at 3557.588 kb on - strand, within Shewana3_2962 at 3557.807 kb on - strand, within Shewana3_2962 at 3557.984 kb on - strand, within Shewana3_2962 at 3558.225 kb on - strand, within Shewana3_2962 at 3558.399 kb on - strand, within Shewana3_2962 at 3558.441 kb on - strand at 3558.671 kb on - strand at 3558.691 kb on + strand at 3558.819 kb on + strand at 3558.819 kb on + strand at 3558.827 kb on - strand at 3558.886 kb on + strand at 3558.943 kb on + strand at 3559.110 kb on + strand, within hsdR at 3559.170 kb on + strand, within hsdR at 3559.403 kb on + strand, within hsdR at 3559.411 kb on - strand, within hsdR at 3559.508 kb on + strand, within hsdR at 3559.514 kb on + strand, within hsdR
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,556,466 - Shewana3_2961 0.44 -1.6 3,556,545 + Shewana3_2961 0.49 +0.0 3,556,572 + Shewana3_2961 0.50 +0.0 3,556,666 - Shewana3_2961 0.56 +0.0 3,556,858 - Shewana3_2961 0.68 -2.3 3,556,865 - Shewana3_2961 0.69 +0.2 3,556,865 - Shewana3_2961 0.69 +0.3 3,556,944 + Shewana3_2961 0.74 -0.5 3,556,947 - Shewana3_2961 0.74 -0.2 3,557,027 - Shewana3_2961 0.79 -0.4 3,557,034 + Shewana3_2961 0.79 +0.0 3,557,042 - Shewana3_2961 0.80 +0.6 3,557,100 - Shewana3_2961 0.84 -0.2 3,557,454 - -0.8 3,557,550 + -1.1 3,557,588 - Shewana3_2962 0.18 +0.4 3,557,807 - Shewana3_2962 0.37 -1.7 3,557,984 - Shewana3_2962 0.53 -0.8 3,558,225 - Shewana3_2962 0.74 -2.3 3,558,399 - Shewana3_2962 0.90 +0.1 3,558,441 - -0.4 3,558,671 - -2.1 3,558,691 + -1.4 3,558,819 + +0.1 3,558,819 + +0.6 3,558,827 - +1.1 3,558,886 + +0.8 3,558,943 + -2.2 3,559,110 + hsdR Shewana3_2963 0.13 -1.7 3,559,170 + hsdR Shewana3_2963 0.15 +0.1 3,559,403 + hsdR Shewana3_2963 0.22 -0.3 3,559,411 - hsdR Shewana3_2963 0.22 -1.1 3,559,508 + hsdR Shewana3_2963 0.25 +0.7 3,559,514 + hsdR Shewana3_2963 0.25 +0.7
Or see this region's nucleotide sequence