Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2948 and Shewana3_2949 are separated by 370 nucleotides Shewana3_2949 and Shewana3_2950 are separated by 75 nucleotides Shewana3_2950 and Shewana3_2951 overlap by 11 nucleotides Shewana3_2951 and smpB are separated by 253 nucleotides
Shewana3_2948: Shewana3_2948 - uroporphyrin-III C/tetrapyrrole methyltransferase (RefSeq), at 3,542,442 to 3,543,233
_2948
Shewana3_2949: Shewana3_2949 - SmpA/OmlA domain-containing protein (RefSeq), at 3,543,604 to 3,544,128
_2949
Shewana3_2950: Shewana3_2950 - hypothetical protein (RefSeq), at 3,544,204 to 3,544,539
_2950
Shewana3_2951: Shewana3_2951 - cyclase/dehydrase (RefSeq), at 3,544,529 to 3,544,966
_2951
Shewana3_2952: smpB - SsrA-binding protein (RefSeq), at 3,545,220 to 3,545,711
smpB
Position (kb) 3544
3545 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 3543.310 kb on - strand at 3543.995 kb on + strand, within Shewana3_2949 at 3544.136 kb on + strand at 3544.202 kb on + strand at 3544.210 kb on - strand at 3544.210 kb on - strand at 3544.220 kb on - strand at 3544.247 kb on + strand, within Shewana3_2950 at 3544.255 kb on - strand, within Shewana3_2950 at 3544.255 kb on - strand, within Shewana3_2950
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,543,310 - +1.0 3,543,995 + Shewana3_2949 0.74 -4.4 3,544,136 + +0.6 3,544,202 + +3.2 3,544,210 - +0.3 3,544,210 - -2.4 3,544,220 - -0.8 3,544,247 + Shewana3_2950 0.13 +1.3 3,544,255 - Shewana3_2950 0.15 -0.0 3,544,255 - Shewana3_2950 0.15 +0.3
Or see this region's nucleotide sequence