Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2775 and Shewana3_2776 are separated by 458 nucleotides Shewana3_2776 and Shewana3_2777 are separated by 98 nucleotides Shewana3_2777 and Shewana3_2778 are separated by 84 nucleotides
Shewana3_2775: Shewana3_2775 - outer membrane protein W (RefSeq), at 3,314,270 to 3,314,926
_2775
Shewana3_2776: Shewana3_2776 - hypothetical protein (RefSeq), at 3,315,385 to 3,315,972
_2776
Shewana3_2777: Shewana3_2777 - maleylacetoacetate isomerase (RefSeq), at 3,316,071 to 3,316,721
_2777
Shewana3_2778: Shewana3_2778 - fumarylacetoacetate (FAA) hydrolase (RefSeq), at 3,316,806 to 3,317,792
_2778
Position (kb)
3315
3316 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3314.447 kb on + strand, within Shewana3_2775 at 3314.447 kb on + strand, within Shewana3_2775 at 3314.465 kb on - strand, within Shewana3_2775 at 3314.600 kb on + strand, within Shewana3_2775 at 3314.600 kb on + strand, within Shewana3_2775 at 3314.659 kb on - strand, within Shewana3_2775 at 3315.074 kb on + strand at 3315.144 kb on + strand at 3315.345 kb on + strand at 3315.374 kb on + strand at 3315.796 kb on + strand, within Shewana3_2776 at 3315.798 kb on + strand, within Shewana3_2776 at 3315.806 kb on - strand, within Shewana3_2776 at 3315.806 kb on - strand, within Shewana3_2776 at 3315.913 kb on - strand, within Shewana3_2776 at 3315.924 kb on - strand at 3315.929 kb on - strand at 3315.946 kb on - strand at 3316.068 kb on + strand at 3316.135 kb on + strand at 3316.135 kb on + strand at 3316.200 kb on - strand, within Shewana3_2777 at 3316.324 kb on + strand, within Shewana3_2777 at 3316.357 kb on + strand, within Shewana3_2777 at 3316.517 kb on - strand, within Shewana3_2777 at 3316.732 kb on + strand at 3316.803 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,314,447 + Shewana3_2775 0.27 +1.3 3,314,447 + Shewana3_2775 0.27 +0.9 3,314,465 - Shewana3_2775 0.30 +1.5 3,314,600 + Shewana3_2775 0.50 +1.2 3,314,600 + Shewana3_2775 0.50 +0.3 3,314,659 - Shewana3_2775 0.59 -1.1 3,315,074 + +0.7 3,315,144 + +0.3 3,315,345 + -3.0 3,315,374 + -1.9 3,315,796 + Shewana3_2776 0.70 +0.6 3,315,798 + Shewana3_2776 0.70 +0.1 3,315,806 - Shewana3_2776 0.72 +0.3 3,315,806 - Shewana3_2776 0.72 +0.4 3,315,913 - Shewana3_2776 0.90 +1.0 3,315,924 - +0.7 3,315,929 - -1.2 3,315,946 - +1.0 3,316,068 + -2.8 3,316,135 + -1.4 3,316,135 + +0.5 3,316,200 - Shewana3_2777 0.20 +1.0 3,316,324 + Shewana3_2777 0.39 -1.1 3,316,357 + Shewana3_2777 0.44 -0.3 3,316,517 - Shewana3_2777 0.69 +0.6 3,316,732 + -1.3 3,316,803 + +1.0
Or see this region's nucleotide sequence