Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2608 and Shewana3_2609 are separated by 125 nucleotides Shewana3_2609 and Shewana3_2610 are separated by 351 nucleotides Shewana3_2610 and Shewana3_2611 are separated by 266 nucleotides
Shewana3_2608: Shewana3_2608 - hypothetical protein (RefSeq), at 3,112,562 to 3,113,071
_2608
Shewana3_2609: Shewana3_2609 - hypothetical protein (RefSeq), at 3,113,197 to 3,113,655
_2609
Shewana3_2610: Shewana3_2610 - peptidase M23B (RefSeq), at 3,114,007 to 3,115,302
_2610
Shewana3_2611: Shewana3_2611 - exonuclease SbcC, putative (RefSeq), at 3,115,569 to 3,118,625
_2611
Position (kb)
3114
3115
3116 Strain fitness (log2 ratio)
-2
-1
0
1 at 3113.371 kb on + strand, within Shewana3_2609 at 3113.583 kb on + strand, within Shewana3_2609 at 3113.760 kb on - strand at 3113.840 kb on - strand at 3113.911 kb on - strand at 3113.921 kb on + strand at 3113.931 kb on - strand at 3114.501 kb on - strand, within Shewana3_2610 at 3115.490 kb on - strand at 3115.726 kb on - strand at 3115.815 kb on + strand at 3115.815 kb on + strand at 3115.887 kb on + strand, within Shewana3_2611 at 3116.218 kb on + strand, within Shewana3_2611 at 3116.218 kb on + strand, within Shewana3_2611 at 3116.226 kb on - strand, within Shewana3_2611 at 3116.291 kb on + strand, within Shewana3_2611
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,113,371 + Shewana3_2609 0.38 +0.3 3,113,583 + Shewana3_2609 0.84 -0.4 3,113,760 - -2.0 3,113,840 - +0.3 3,113,911 - -1.5 3,113,921 + -1.2 3,113,931 - -0.5 3,114,501 - Shewana3_2610 0.38 +1.1 3,115,490 - -0.7 3,115,726 - +0.5 3,115,815 + -1.8 3,115,815 + -0.2 3,115,887 + Shewana3_2611 0.10 +0.1 3,116,218 + Shewana3_2611 0.21 +0.3 3,116,218 + Shewana3_2611 0.21 +0.3 3,116,226 - Shewana3_2611 0.21 +0.3 3,116,291 + Shewana3_2611 0.24 -1.8
Or see this region's nucleotide sequence