Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2573 and Shewana3_2574 are separated by 83 nucleotides Shewana3_2574 and Shewana3_2575 overlap by 20 nucleotides Shewana3_2575 and Shewana3_2576 are separated by 73 nucleotides Shewana3_2576 and Shewana3_2577 are separated by 350 nucleotides Shewana3_2577 and Shewana3_2578 are separated by 230 nucleotides
Shewana3_2573: Shewana3_2573 - Cytidine deaminase (EC 3.5.4.5) (from data) , at 3,077,679 to 3,078,569
_2573
Shewana3_2574: Shewana3_2574 - hypothetical protein (RefSeq), at 3,078,653 to 3,078,868
_2574
Shewana3_2575: Shewana3_2575 - glyoxalase/bleomycin resistance protein/dioxygenase (RefSeq), at 3,078,849 to 3,079,196
_2575
Shewana3_2576: Shewana3_2576 - hypothetical protein (RefSeq), at 3,079,270 to 3,079,974
_2576
Shewana3_2577: Shewana3_2577 - glyoxalase family protein (RefSeq), at 3,080,325 to 3,080,705
_2577
Shewana3_2578: Shewana3_2578 - hypothetical protein (RefSeq), at 3,080,936 to 3,084,166
_2578
Position (kb)
3079
3080 Strain fitness (log2 ratio)
-2
-1
0
1 at 3078.276 kb on + strand, within Shewana3_2573 at 3078.284 kb on - strand, within Shewana3_2573 at 3078.374 kb on + strand, within Shewana3_2573 at 3078.469 kb on + strand, within Shewana3_2573 at 3078.584 kb on + strand at 3078.653 kb on - strand at 3078.851 kb on + strand at 3079.281 kb on - strand at 3079.731 kb on - strand, within Shewana3_2576 at 3079.818 kb on - strand, within Shewana3_2576 at 3079.842 kb on - strand, within Shewana3_2576 at 3079.845 kb on - strand, within Shewana3_2576 at 3079.889 kb on + strand, within Shewana3_2576 at 3079.912 kb on - strand at 3079.964 kb on + strand at 3079.985 kb on + strand at 3080.019 kb on + strand at 3080.138 kb on - strand at 3080.761 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,078,276 + Shewana3_2573 0.67 +1.5 3,078,284 - Shewana3_2573 0.68 -0.3 3,078,374 + Shewana3_2573 0.78 +0.2 3,078,469 + Shewana3_2573 0.89 -0.9 3,078,584 + -1.8 3,078,653 - +1.1 3,078,851 + -1.0 3,079,281 - +0.8 3,079,731 - Shewana3_2576 0.65 -2.3 3,079,818 - Shewana3_2576 0.78 -1.5 3,079,842 - Shewana3_2576 0.81 +0.1 3,079,845 - Shewana3_2576 0.82 -0.0 3,079,889 + Shewana3_2576 0.88 -1.7 3,079,912 - +0.2 3,079,964 + +0.7 3,079,985 + -2.0 3,080,019 + +0.2 3,080,138 - -0.7 3,080,761 - -1.9
Or see this region's nucleotide sequence