Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2414 and Shewana3_2415 are separated by 112 nucleotides Shewana3_2415 and Shewana3_2416 are separated by 9 nucleotides Shewana3_2416 and Shewana3_2417 are separated by 324 nucleotides
Shewana3_2414: Shewana3_2414 - cystathionine beta-lyase (RefSeq), at 2,895,136 to 2,896,335
_2414
Shewana3_2415: Shewana3_2415 - CreA family protein (RefSeq), at 2,896,448 to 2,896,963
_2415
Shewana3_2416: Shewana3_2416 - putative chaperone (RefSeq), at 2,896,973 to 2,898,427
_2416
Shewana3_2417: Shewana3_2417 - polyphosphate kinase (RefSeq), at 2,898,752 to 2,900,914
_2417
Position (kb)
2896
2897
2898
2899 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2896.302 kb on + strand at 2896.367 kb on + strand at 2896.489 kb on + strand at 2896.996 kb on - strand at 2897.086 kb on + strand at 2897.551 kb on + strand, within Shewana3_2416 at 2897.607 kb on + strand, within Shewana3_2416 at 2897.659 kb on + strand, within Shewana3_2416 at 2897.780 kb on - strand, within Shewana3_2416 at 2897.792 kb on - strand, within Shewana3_2416 at 2897.954 kb on - strand, within Shewana3_2416 at 2898.027 kb on - strand, within Shewana3_2416 at 2898.161 kb on - strand, within Shewana3_2416 at 2898.221 kb on - strand, within Shewana3_2416 at 2898.350 kb on + strand at 2898.358 kb on - strand at 2898.603 kb on - strand at 2898.716 kb on - strand at 2898.742 kb on - strand at 2898.933 kb on - strand at 2899.236 kb on - strand, within Shewana3_2417 at 2899.267 kb on - strand, within Shewana3_2417 at 2899.267 kb on - strand, within Shewana3_2417 at 2899.341 kb on + strand, within Shewana3_2417 at 2899.374 kb on + strand, within Shewana3_2417
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,896,302 + +0.5 2,896,367 + +1.2 2,896,489 + +0.3 2,896,996 - +0.5 2,897,086 + +0.6 2,897,551 + Shewana3_2416 0.40 -1.8 2,897,607 + Shewana3_2416 0.44 -3.2 2,897,659 + Shewana3_2416 0.47 -0.8 2,897,780 - Shewana3_2416 0.55 -0.3 2,897,792 - Shewana3_2416 0.56 +0.3 2,897,954 - Shewana3_2416 0.67 +0.4 2,898,027 - Shewana3_2416 0.72 +0.5 2,898,161 - Shewana3_2416 0.82 -2.3 2,898,221 - Shewana3_2416 0.86 -0.0 2,898,350 + -1.3 2,898,358 - -0.1 2,898,603 - +1.2 2,898,716 - +0.6 2,898,742 - -2.1 2,898,933 - +0.6 2,899,236 - Shewana3_2417 0.22 +0.5 2,899,267 - Shewana3_2417 0.24 -3.1 2,899,267 - Shewana3_2417 0.24 -2.1 2,899,341 + Shewana3_2417 0.27 -1.8 2,899,374 + Shewana3_2417 0.29 +0.6
Or see this region's nucleotide sequence