Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2400 and Shewana3_2401 are separated by 101 nucleotides Shewana3_2401 and Shewana3_2402 are separated by 252 nucleotides Shewana3_2402 and Shewana3_2403 are separated by 151 nucleotides
Shewana3_2400: Shewana3_2400 - hypothetical protein (RefSeq), at 2,878,568 to 2,879,590
_2400
Shewana3_2401: Shewana3_2401 - DNA ligase (RefSeq), at 2,879,692 to 2,880,606
_2401
Shewana3_2402: Shewana3_2402 - diacylglycerol kinase (RefSeq), at 2,880,859 to 2,881,230
_2402
Shewana3_2403: Shewana3_2403 - LysR family transcriptional regulator (RefSeq), at 2,881,382 to 2,882,239
_2403
Position (kb)
2879
2880
2881 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2879.019 kb on - strand, within Shewana3_2400 at 2879.157 kb on - strand, within Shewana3_2400 at 2879.340 kb on + strand, within Shewana3_2400 at 2879.452 kb on + strand, within Shewana3_2400 at 2879.470 kb on + strand, within Shewana3_2400 at 2880.003 kb on - strand, within Shewana3_2401 at 2880.026 kb on - strand, within Shewana3_2401 at 2880.034 kb on + strand, within Shewana3_2401 at 2880.042 kb on - strand, within Shewana3_2401 at 2880.189 kb on - strand, within Shewana3_2401 at 2880.355 kb on - strand, within Shewana3_2401 at 2880.816 kb on + strand at 2881.183 kb on - strand, within Shewana3_2402 at 2881.190 kb on - strand, within Shewana3_2402 at 2881.194 kb on + strand at 2881.388 kb on + strand at 2881.388 kb on - strand at 2881.450 kb on + strand at 2881.476 kb on - strand, within Shewana3_2403 at 2881.476 kb on - strand, within Shewana3_2403
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,879,019 - Shewana3_2400 0.44 +0.4 2,879,157 - Shewana3_2400 0.58 -2.0 2,879,340 + Shewana3_2400 0.75 -0.8 2,879,452 + Shewana3_2400 0.86 -2.0 2,879,470 + Shewana3_2400 0.88 +0.4 2,880,003 - Shewana3_2401 0.34 +0.2 2,880,026 - Shewana3_2401 0.37 +0.6 2,880,034 + Shewana3_2401 0.37 -2.7 2,880,042 - Shewana3_2401 0.38 +0.4 2,880,189 - Shewana3_2401 0.54 -1.8 2,880,355 - Shewana3_2401 0.72 -1.3 2,880,816 + -2.9 2,881,183 - Shewana3_2402 0.87 -1.1 2,881,190 - Shewana3_2402 0.89 -0.6 2,881,194 + -1.0 2,881,388 + +0.9 2,881,388 - +0.3 2,881,450 + -0.8 2,881,476 - Shewana3_2403 0.11 -0.5 2,881,476 - Shewana3_2403 0.11 +0.3
Or see this region's nucleotide sequence