Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2295 and Shewana3_2296 are separated by 111 nucleotides Shewana3_2296 and Shewana3_2297 are separated by 399 nucleotides Shewana3_2297 and Shewana3_2298 are separated by 101 nucleotides
Shewana3_2295: Shewana3_2295 - L-serine ammonia-lyase (RefSeq), at 2,758,463 to 2,759,839
_2295
Shewana3_2296: Shewana3_2296 - hypothetical protein (RefSeq), at 2,759,951 to 2,760,688
_2296
Shewana3_2297: Shewana3_2297 - ISPsy11, transposase OrfA (RefSeq), at 2,761,088 to 2,761,480
_2297
Shewana3_2298: Shewana3_2298 - integrase catalytic subunit (RefSeq), at 2,761,582 to 2,762,304
_2298
Position (kb)
2759
2760
2761 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2759.061 kb on + strand, within Shewana3_2295 at 2759.164 kb on + strand, within Shewana3_2295 at 2759.373 kb on - strand, within Shewana3_2295 at 2759.606 kb on + strand, within Shewana3_2295 at 2759.702 kb on + strand at 2759.833 kb on + strand at 2760.051 kb on - strand, within Shewana3_2296 at 2760.076 kb on + strand, within Shewana3_2296 at 2760.084 kb on - strand, within Shewana3_2296 at 2760.084 kb on - strand, within Shewana3_2296 at 2760.094 kb on - strand, within Shewana3_2296 at 2760.110 kb on + strand, within Shewana3_2296 at 2760.165 kb on - strand, within Shewana3_2296 at 2760.464 kb on - strand, within Shewana3_2296 at 2760.466 kb on - strand, within Shewana3_2296 at 2760.559 kb on - strand, within Shewana3_2296 at 2760.860 kb on + strand at 2760.964 kb on + strand at 2760.964 kb on + strand at 2760.972 kb on - strand at 2761.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,759,061 + Shewana3_2295 0.43 +2.3 2,759,164 + Shewana3_2295 0.51 -0.7 2,759,373 - Shewana3_2295 0.66 +1.9 2,759,606 + Shewana3_2295 0.83 +2.0 2,759,702 + -0.8 2,759,833 + +0.5 2,760,051 - Shewana3_2296 0.14 +0.8 2,760,076 + Shewana3_2296 0.17 +0.4 2,760,084 - Shewana3_2296 0.18 -1.4 2,760,084 - Shewana3_2296 0.18 +0.2 2,760,094 - Shewana3_2296 0.19 +0.4 2,760,110 + Shewana3_2296 0.22 -0.8 2,760,165 - Shewana3_2296 0.29 +0.3 2,760,464 - Shewana3_2296 0.70 -2.6 2,760,466 - Shewana3_2296 0.70 +0.8 2,760,559 - Shewana3_2296 0.82 -0.9 2,760,860 + -0.1 2,760,964 + +0.3 2,760,964 + -1.9 2,760,972 - +0.5 2,761,050 - +0.2
Or see this region's nucleotide sequence