Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1884 and Shewana3_1885 are separated by 10 nucleotides Shewana3_1885 and Shewana3_1886 are separated by 113 nucleotides Shewana3_1886 and mdoG are separated by 444 nucleotides
Shewana3_1884: Shewana3_1884 - ApbE family lipoprotein (RefSeq), at 2,231,229 to 2,232,131
_1884
Shewana3_1885: Shewana3_1885 - two component transcriptional regulator (RefSeq), at 2,232,142 to 2,232,801
_1885
Shewana3_1886: Shewana3_1886 - ATPase domain-containing protein (RefSeq), at 2,232,915 to 2,234,309
_1886
Shewana3_1887: mdoG - glucan biosynthesis protein G (RefSeq), at 2,234,754 to 2,236,391
mdoG
Position (kb)
2232
2233
2234
2235 Strain fitness (log2 ratio)
-2
-1
0
1 at 2232.065 kb on + strand at 2232.132 kb on + strand at 2232.192 kb on - strand at 2232.231 kb on + strand, within Shewana3_1885 at 2232.236 kb on - strand, within Shewana3_1885 at 2232.350 kb on - strand, within Shewana3_1885 at 2232.658 kb on + strand, within Shewana3_1885 at 2232.744 kb on - strand at 2232.761 kb on - strand at 2232.845 kb on + strand at 2232.930 kb on + strand at 2233.195 kb on + strand, within Shewana3_1886 at 2233.470 kb on + strand, within Shewana3_1886 at 2233.583 kb on + strand, within Shewana3_1886 at 2233.587 kb on - strand, within Shewana3_1886 at 2233.598 kb on + strand, within Shewana3_1886 at 2233.606 kb on - strand, within Shewana3_1886 at 2233.670 kb on + strand, within Shewana3_1886 at 2233.751 kb on + strand, within Shewana3_1886 at 2233.771 kb on + strand, within Shewana3_1886 at 2233.880 kb on - strand, within Shewana3_1886 at 2234.101 kb on - strand, within Shewana3_1886 at 2234.123 kb on + strand, within Shewana3_1886 at 2234.254 kb on + strand at 2234.285 kb on - strand at 2234.298 kb on - strand at 2234.892 kb on + strand at 2234.913 kb on - strand at 2235.075 kb on - strand, within mdoG at 2235.233 kb on + strand, within mdoG
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,232,065 + -0.7 2,232,132 + +0.1 2,232,192 - +0.2 2,232,231 + Shewana3_1885 0.13 -2.0 2,232,236 - Shewana3_1885 0.14 -0.3 2,232,350 - Shewana3_1885 0.32 +0.3 2,232,658 + Shewana3_1885 0.78 -1.6 2,232,744 - +0.5 2,232,761 - -0.8 2,232,845 + -1.1 2,232,930 + +0.3 2,233,195 + Shewana3_1886 0.20 +0.9 2,233,470 + Shewana3_1886 0.40 -1.7 2,233,583 + Shewana3_1886 0.48 -1.4 2,233,587 - Shewana3_1886 0.48 +0.4 2,233,598 + Shewana3_1886 0.49 +0.2 2,233,606 - Shewana3_1886 0.50 +0.6 2,233,670 + Shewana3_1886 0.54 -1.5 2,233,751 + Shewana3_1886 0.60 -2.0 2,233,771 + Shewana3_1886 0.61 -1.4 2,233,880 - Shewana3_1886 0.69 +0.5 2,234,101 - Shewana3_1886 0.85 -0.9 2,234,123 + Shewana3_1886 0.87 +0.2 2,234,254 + -1.2 2,234,285 - +0.3 2,234,298 - +0.7 2,234,892 + -1.5 2,234,913 - +0.3 2,235,075 - mdoG Shewana3_1887 0.20 -2.0 2,235,233 + mdoG Shewana3_1887 0.29 +0.2
Or see this region's nucleotide sequence