Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1723 and Shewana3_1724 overlap by 10 nucleotides Shewana3_1724 and Shewana3_1725 are separated by 164 nucleotides Shewana3_1725 and Shewana3_1726 are separated by 156 nucleotides
Shewana3_1723: Shewana3_1723 - ATPase domain-containing protein (RefSeq), at 2,026,085 to 2,027,434
_1723
Shewana3_1724: Shewana3_1724 - two component transcriptional regulator (RefSeq), at 2,027,425 to 2,028,108
_1724
Shewana3_1725: Shewana3_1725 - hypothetical protein (RefSeq), at 2,028,273 to 2,028,620
_1725
Shewana3_1726: Shewana3_1726 - sodium:dicarboxylate symporter (RefSeq), at 2,028,777 to 2,030,090
_1726
Position (kb)
2027
2028
2029 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2026.538 kb on + strand, within Shewana3_1723 at 2026.875 kb on + strand, within Shewana3_1723 at 2027.063 kb on + strand, within Shewana3_1723 at 2027.472 kb on + strand at 2027.480 kb on - strand at 2027.480 kb on - strand at 2027.531 kb on - strand, within Shewana3_1724 at 2027.724 kb on - strand, within Shewana3_1724 at 2028.118 kb on - strand at 2028.212 kb on - strand at 2028.421 kb on - strand, within Shewana3_1725 at 2028.491 kb on - strand, within Shewana3_1725 at 2029.073 kb on - strand, within Shewana3_1726
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,026,538 + Shewana3_1723 0.34 -0.4 2,026,875 + Shewana3_1723 0.59 -4.6 2,027,063 + Shewana3_1723 0.72 -1.6 2,027,472 + -3.9 2,027,480 - -1.8 2,027,480 - -2.6 2,027,531 - Shewana3_1724 0.15 -2.6 2,027,724 - Shewana3_1724 0.44 -4.1 2,028,118 - +1.0 2,028,212 - -1.0 2,028,421 - Shewana3_1725 0.43 -2.0 2,028,491 - Shewana3_1725 0.63 -2.0 2,029,073 - Shewana3_1726 0.23 +0.2
Or see this region's nucleotide sequence