Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1604 and Shewana3_1605 are separated by 187 nucleotides Shewana3_1605 and Shewana3_1606 are separated by 107 nucleotides Shewana3_1606 and Shewana3_1607 are separated by 74 nucleotides
Shewana3_1604: Shewana3_1604 - hypothetical protein (RefSeq), at 1,882,924 to 1,884,747
_1604
Shewana3_1605: Shewana3_1605 - ribosome modulation factor (RefSeq), at 1,884,935 to 1,885,111
_1605
Shewana3_1606: Shewana3_1606 - 3-hydroxydecanoyl-(acyl carrier protein) dehydratase (RefSeq), at 1,885,219 to 1,885,734
_1606
Shewana3_1607: Shewana3_1607 - ATP-dependent protease (RefSeq), at 1,885,809 to 1,887,557
_1607
Position (kb)
1884
1885
1886 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1884.392 kb on + strand, within Shewana3_1604 at 1884.587 kb on + strand at 1884.643 kb on + strand at 1884.918 kb on + strand at 1885.013 kb on - strand, within Shewana3_1605 at 1885.051 kb on + strand, within Shewana3_1605 at 1885.150 kb on + strand at 1885.215 kb on - strand at 1885.320 kb on - strand, within Shewana3_1606 at 1885.386 kb on - strand, within Shewana3_1606 at 1885.417 kb on - strand, within Shewana3_1606 at 1885.417 kb on - strand, within Shewana3_1606 at 1885.434 kb on - strand, within Shewana3_1606
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,884,392 + Shewana3_1604 0.80 +1.8 1,884,587 + +2.5 1,884,643 + +1.6 1,884,918 + +0.4 1,885,013 - Shewana3_1605 0.44 -2.2 1,885,051 + Shewana3_1605 0.66 -1.3 1,885,150 + +0.0 1,885,215 - -0.6 1,885,320 - Shewana3_1606 0.20 +0.1 1,885,386 - Shewana3_1606 0.32 -3.0 1,885,417 - Shewana3_1606 0.38 +0.3 1,885,417 - Shewana3_1606 0.38 +0.3 1,885,434 - Shewana3_1606 0.42 -1.3
Or see this region's nucleotide sequence