Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1465 and Shewana3_1466 are separated by 169 nucleotides Shewana3_1466 and Shewana3_1467 are separated by 65 nucleotides Shewana3_1467 and Shewana3_1468 are separated by 80 nucleotides
Shewana3_1465: Shewana3_1465 - peptidase M16 domain-containing protein (RefSeq), at 1,702,658 to 1,705,447
_1465
Shewana3_1466: Shewana3_1466 - phosphohistidine phosphatase, SixA (RefSeq), at 1,705,617 to 1,706,087
_1466
Shewana3_1467: Shewana3_1467 - hypothetical protein (RefSeq), at 1,706,153 to 1,706,683
_1467
Shewana3_1468: Shewana3_1468 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase (RefSeq), at 1,706,764 to 1,707,708
_1468
Position (kb)
1705
1706
1707 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1704.654 kb on - strand, within Shewana3_1465 at 1704.743 kb on - strand, within Shewana3_1465 at 1704.762 kb on - strand, within Shewana3_1465 at 1704.854 kb on + strand, within Shewana3_1465 at 1704.885 kb on + strand, within Shewana3_1465 at 1704.893 kb on - strand, within Shewana3_1465 at 1704.893 kb on - strand, within Shewana3_1465 at 1704.979 kb on + strand, within Shewana3_1465 at 1705.057 kb on + strand, within Shewana3_1465 at 1705.158 kb on + strand, within Shewana3_1465 at 1705.432 kb on + strand at 1705.673 kb on + strand, within Shewana3_1466 at 1705.710 kb on + strand, within Shewana3_1466 at 1705.718 kb on - strand, within Shewana3_1466 at 1705.758 kb on + strand, within Shewana3_1466 at 1705.910 kb on + strand, within Shewana3_1466 at 1706.233 kb on - strand, within Shewana3_1467 at 1706.238 kb on + strand, within Shewana3_1467 at 1706.343 kb on - strand, within Shewana3_1467 at 1706.345 kb on + strand, within Shewana3_1467 at 1706.353 kb on - strand, within Shewana3_1467 at 1706.797 kb on + strand at 1706.807 kb on + strand at 1706.813 kb on - strand at 1706.815 kb on - strand at 1706.941 kb on + strand, within Shewana3_1468
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,704,654 - Shewana3_1465 0.72 +0.0 1,704,743 - Shewana3_1465 0.75 -0.3 1,704,762 - Shewana3_1465 0.75 +1.0 1,704,854 + Shewana3_1465 0.79 -0.1 1,704,885 + Shewana3_1465 0.80 +0.1 1,704,893 - Shewana3_1465 0.80 -2.1 1,704,893 - Shewana3_1465 0.80 +0.3 1,704,979 + Shewana3_1465 0.83 -1.5 1,705,057 + Shewana3_1465 0.86 -1.6 1,705,158 + Shewana3_1465 0.90 +1.6 1,705,432 + +0.5 1,705,673 + Shewana3_1466 0.12 -0.4 1,705,710 + Shewana3_1466 0.20 -1.6 1,705,718 - Shewana3_1466 0.21 -1.8 1,705,758 + Shewana3_1466 0.30 -2.2 1,705,910 + Shewana3_1466 0.62 -2.9 1,706,233 - Shewana3_1467 0.15 +1.6 1,706,238 + Shewana3_1467 0.16 +0.9 1,706,343 - Shewana3_1467 0.36 -1.2 1,706,345 + Shewana3_1467 0.36 -0.6 1,706,353 - Shewana3_1467 0.38 +0.8 1,706,797 + -2.8 1,706,807 + -0.5 1,706,813 - -2.5 1,706,815 - -0.0 1,706,941 + Shewana3_1468 0.19 -0.6
Or see this region's nucleotide sequence