Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1304 and Shewana3_1305 are separated by 61 nucleotides Shewana3_1305 and Shewana3_1306 are separated by 1 nucleotides Shewana3_1306 and Shewana3_1307 are separated by 2 nucleotides
Shewana3_1304: Shewana3_1304 - N-acetylneuraminate synthase (RefSeq), at 1,519,014 to 1,520,066
_1304
Shewana3_1305: Shewana3_1305 - hypothetical protein (RefSeq), at 1,520,128 to 1,520,940
_1305
Shewana3_1306: Shewana3_1306 - hypothetical protein (RefSeq), at 1,520,942 to 1,521,835
_1306
Shewana3_1307: Shewana3_1307 - hypothetical protein (RefSeq), at 1,521,838 to 1,522,539
_1307
Position (kb)
1520
1521 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1519.394 kb on - strand, within Shewana3_1304 at 1519.495 kb on - strand, within Shewana3_1304 at 1519.498 kb on - strand, within Shewana3_1304 at 1519.592 kb on - strand, within Shewana3_1304 at 1519.836 kb on + strand, within Shewana3_1304 at 1519.975 kb on + strand at 1519.975 kb on + strand at 1520.103 kb on - strand at 1520.126 kb on + strand at 1520.134 kb on - strand at 1520.317 kb on + strand, within Shewana3_1305 at 1520.397 kb on - strand, within Shewana3_1305 at 1520.561 kb on - strand, within Shewana3_1305 at 1521.018 kb on - strand at 1521.390 kb on - strand, within Shewana3_1306 at 1521.469 kb on + strand, within Shewana3_1306 at 1521.607 kb on - strand, within Shewana3_1306 at 1521.767 kb on - strand at 1521.862 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,519,394 - Shewana3_1304 0.36 +0.1 1,519,495 - Shewana3_1304 0.46 -0.1 1,519,498 - Shewana3_1304 0.46 -1.0 1,519,592 - Shewana3_1304 0.55 -0.1 1,519,836 + Shewana3_1304 0.78 -0.3 1,519,975 + +1.3 1,519,975 + +0.6 1,520,103 - +1.2 1,520,126 + -0.0 1,520,134 - +0.1 1,520,317 + Shewana3_1305 0.23 +0.5 1,520,397 - Shewana3_1305 0.33 +0.7 1,520,561 - Shewana3_1305 0.53 +0.2 1,521,018 - +0.2 1,521,390 - Shewana3_1306 0.50 +0.4 1,521,469 + Shewana3_1306 0.59 -2.8 1,521,607 - Shewana3_1306 0.74 +0.7 1,521,767 - +0.6 1,521,862 + +0.8
Or see this region's nucleotide sequence