Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1226 and Shewana3_1227 are separated by 32 nucleotides Shewana3_1227 and Shewana3_1228 overlap by 4 nucleotides Shewana3_1228 and ispG are separated by 42 nucleotides
Shewana3_1226: Shewana3_1226 - hypothetical protein (RefSeq), at 1,429,449 to 1,430,570
_1226
Shewana3_1227: Shewana3_1227 - type IV pilus biogenesis/stability protein PilW (RefSeq), at 1,430,603 to 1,431,391
_1227
Shewana3_1228: Shewana3_1228 - hypothetical protein (RefSeq), at 1,431,388 to 1,432,515
_1228
Shewana3_1229: ispG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (RefSeq), at 1,432,558 to 1,433,673
ispG
Position (kb)
1431
1432
1433 Strain fitness (log2 ratio)
-2
-1
0
1 at 1430.821 kb on - strand, within Shewana3_1227 at 1430.925 kb on - strand, within Shewana3_1227 at 1431.499 kb on + strand at 1431.941 kb on + strand, within Shewana3_1228 at 1432.157 kb on + strand, within Shewana3_1228 at 1432.192 kb on + strand, within Shewana3_1228 at 1432.192 kb on + strand, within Shewana3_1228 at 1432.224 kb on + strand, within Shewana3_1228 at 1432.226 kb on + strand, within Shewana3_1228 at 1432.243 kb on + strand, within Shewana3_1228 at 1432.248 kb on + strand, within Shewana3_1228 at 1432.290 kb on + strand, within Shewana3_1228 at 1432.349 kb on + strand, within Shewana3_1228 at 1432.377 kb on + strand, within Shewana3_1228
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,430,821 - Shewana3_1227 0.28 +0.6 1,430,925 - Shewana3_1227 0.41 -0.5 1,431,499 + -2.4 1,431,941 + Shewana3_1228 0.49 +0.9 1,432,157 + Shewana3_1228 0.68 -1.2 1,432,192 + Shewana3_1228 0.71 +1.5 1,432,192 + Shewana3_1228 0.71 +0.8 1,432,224 + Shewana3_1228 0.74 +0.2 1,432,226 + Shewana3_1228 0.74 +1.1 1,432,243 + Shewana3_1228 0.76 +0.1 1,432,248 + Shewana3_1228 0.76 +1.1 1,432,290 + Shewana3_1228 0.80 -0.0 1,432,349 + Shewana3_1228 0.85 +0.5 1,432,377 + Shewana3_1228 0.88 -0.1
Or see this region's nucleotide sequence