Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0051 and Shewana3_R0052 are separated by 6 nucleotides Shewana3_R0052 and Shewana3_1178 are separated by 388 nucleotides Shewana3_1178 and Shewana3_1179 are separated by 4 nucleotides Shewana3_1179 and Shewana3_1180 are separated by 84 nucleotides Shewana3_1180 and Shewana3_1181 are separated by 9 nucleotides
Shewana3_R0051: Shewana3_R0051 - tRNA-Thr (RefSeq), at 1,386,491 to 1,386,566
_R0051
Shewana3_R0052: Shewana3_R0052 - tRNA-Phe (RefSeq), at 1,386,573 to 1,386,648
_R0052
Shewana3_1178: Shewana3_1178 - cytochrome B561 (RefSeq), at 1,387,037 to 1,387,588
_1178
Shewana3_1179: Shewana3_1179 - hypothetical protein (RefSeq), at 1,387,593 to 1,388,168
_1179
Shewana3_1180: Shewana3_1180 - hypothetical protein (RefSeq), at 1,388,253 to 1,388,531
_1180
Shewana3_1181: Shewana3_1181 - A/G-specific DNA-adenine glycosylase (RefSeq), at 1,388,541 to 1,389,659
_1181
Position (kb)
1387
1388 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1386.170 kb on - strand at 1386.227 kb on - strand at 1386.294 kb on + strand at 1386.302 kb on - strand at 1386.373 kb on + strand at 1386.426 kb on + strand at 1387.029 kb on - strand at 1387.029 kb on - strand at 1387.039 kb on - strand at 1387.195 kb on + strand, within Shewana3_1178 at 1387.252 kb on + strand, within Shewana3_1178 at 1387.294 kb on + strand, within Shewana3_1178 at 1387.558 kb on + strand at 1388.174 kb on + strand at 1388.250 kb on + strand at 1388.303 kb on - strand, within Shewana3_1180 at 1388.348 kb on + strand, within Shewana3_1180 at 1388.461 kb on + strand, within Shewana3_1180 at 1388.472 kb on - strand, within Shewana3_1180
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,386,170 - -0.8 1,386,227 - +1.7 1,386,294 + +0.3 1,386,302 - -1.9 1,386,373 + +0.3 1,386,426 + +0.2 1,387,029 - -1.3 1,387,029 - +0.6 1,387,039 - +0.1 1,387,195 + Shewana3_1178 0.29 +0.6 1,387,252 + Shewana3_1178 0.39 +0.9 1,387,294 + Shewana3_1178 0.47 +0.7 1,387,558 + +0.4 1,388,174 + +0.7 1,388,250 + +0.5 1,388,303 - Shewana3_1180 0.18 -1.8 1,388,348 + Shewana3_1180 0.34 -4.0 1,388,461 + Shewana3_1180 0.75 -4.4 1,388,472 - Shewana3_1180 0.78 -3.0
Or see this region's nucleotide sequence