Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuS and Shewana3_1002 are separated by 121 nucleotides Shewana3_1002 and Shewana3_1003 are separated by 61 nucleotides Shewana3_1003 and Shewana3_1004 are separated by 15 nucleotides
Shewana3_1001: leuS - leucyl-tRNA synthetase (RefSeq), at 1,170,450 to 1,173,029
leuS
Shewana3_1002: Shewana3_1002 - hypothetical protein (RefSeq), at 1,173,151 to 1,173,651
_1002
Shewana3_1003: Shewana3_1003 - methylation site containing protein (RefSeq), at 1,173,713 to 1,174,207
_1003
Shewana3_1004: Shewana3_1004 - apolipoprotein N-acyltransferase (RefSeq), at 1,174,223 to 1,175,773
_1004
Position (kb)
1173
1174
1175 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1173.108 kb on - strand at 1173.383 kb on + strand, within Shewana3_1002 at 1173.383 kb on + strand, within Shewana3_1002 at 1173.383 kb on + strand, within Shewana3_1002 at 1173.383 kb on + strand, within Shewana3_1002 at 1173.403 kb on - strand, within Shewana3_1002 at 1173.482 kb on + strand, within Shewana3_1002 at 1173.496 kb on - strand, within Shewana3_1002 at 1173.503 kb on + strand, within Shewana3_1002 at 1173.530 kb on + strand, within Shewana3_1002 at 1173.544 kb on - strand, within Shewana3_1002 at 1173.605 kb on - strand at 1173.719 kb on + strand at 1173.719 kb on + strand at 1173.727 kb on - strand at 1173.849 kb on - strand, within Shewana3_1003 at 1174.092 kb on + strand, within Shewana3_1003 at 1174.237 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,173,108 - +1.1 1,173,383 + Shewana3_1002 0.46 -1.6 1,173,383 + Shewana3_1002 0.46 +1.4 1,173,383 + Shewana3_1002 0.46 +0.3 1,173,383 + Shewana3_1002 0.46 -1.4 1,173,403 - Shewana3_1002 0.50 +1.8 1,173,482 + Shewana3_1002 0.66 -2.0 1,173,496 - Shewana3_1002 0.69 -1.3 1,173,503 + Shewana3_1002 0.70 +0.6 1,173,530 + Shewana3_1002 0.76 -0.3 1,173,544 - Shewana3_1002 0.78 -0.1 1,173,605 - -0.5 1,173,719 + -1.0 1,173,719 + +0.3 1,173,727 - -0.4 1,173,849 - Shewana3_1003 0.27 +0.6 1,174,092 + Shewana3_1003 0.77 +0.5 1,174,237 + +1.4
Or see this region's nucleotide sequence