Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0975 and Shewana3_0976 are separated by 78 nucleotides Shewana3_0976 and Shewana3_0977 are separated by 468 nucleotides Shewana3_0977 and Shewana3_0978 are separated by 91 nucleotides
Shewana3_0975: Shewana3_0975 - hypothetical protein (RefSeq), at 1,145,808 to 1,146,689
_0975
Shewana3_0976: Shewana3_0976 - putative lipoprotein (RefSeq), at 1,146,768 to 1,147,220
_0976
Shewana3_0977: Shewana3_0977 - SAM-dependent methyltransferase (RefSeq), at 1,147,689 to 1,148,879
_0977
Shewana3_0978: Shewana3_0978 - ribose-5-phosphate isomerase A (RefSeq), at 1,148,971 to 1,149,630
_0978
Position (kb)
1147
1148
1149 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1146.698 kb on - strand at 1146.698 kb on - strand at 1146.856 kb on - strand, within Shewana3_0976 at 1146.866 kb on - strand, within Shewana3_0976 at 1146.866 kb on - strand, within Shewana3_0976 at 1146.890 kb on - strand, within Shewana3_0976 at 1146.993 kb on - strand, within Shewana3_0976 at 1146.994 kb on + strand, within Shewana3_0976 at 1147.044 kb on - strand, within Shewana3_0976 at 1147.096 kb on - strand, within Shewana3_0976 at 1147.125 kb on + strand, within Shewana3_0976 at 1147.181 kb on + strand at 1147.783 kb on - strand at 1148.078 kb on - strand, within Shewana3_0977 at 1148.198 kb on - strand, within Shewana3_0977 at 1148.310 kb on - strand, within Shewana3_0977 at 1148.310 kb on - strand, within Shewana3_0977 at 1148.453 kb on + strand, within Shewana3_0977 at 1148.720 kb on - strand, within Shewana3_0977 at 1148.850 kb on + strand at 1148.890 kb on + strand at 1148.991 kb on - strand at 1149.757 kb on - strand at 1149.757 kb on - strand at 1149.852 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,146,698 - -1.9 1,146,698 - +0.6 1,146,856 - Shewana3_0976 0.19 +0.3 1,146,866 - Shewana3_0976 0.22 +0.6 1,146,866 - Shewana3_0976 0.22 -0.7 1,146,890 - Shewana3_0976 0.27 +0.6 1,146,993 - Shewana3_0976 0.50 +0.3 1,146,994 + Shewana3_0976 0.50 +0.9 1,147,044 - Shewana3_0976 0.61 +0.3 1,147,096 - Shewana3_0976 0.72 +0.1 1,147,125 + Shewana3_0976 0.79 -0.8 1,147,181 + +0.5 1,147,783 - -1.5 1,148,078 - Shewana3_0977 0.33 -2.1 1,148,198 - Shewana3_0977 0.43 -0.7 1,148,310 - Shewana3_0977 0.52 -0.6 1,148,310 - Shewana3_0977 0.52 +0.1 1,148,453 + Shewana3_0977 0.64 -1.8 1,148,720 - Shewana3_0977 0.87 +0.6 1,148,850 + -0.2 1,148,890 + -1.2 1,148,991 - -4.8 1,149,757 - +0.0 1,149,757 - +0.4 1,149,852 + -2.3
Or see this region's nucleotide sequence