Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0972 and Shewana3_0973 are separated by 680 nucleotides Shewana3_0973 and Shewana3_0974 are separated by 11 nucleotides Shewana3_0974 and Shewana3_0975 overlap by 4 nucleotides
Shewana3_0972: Shewana3_0972 - hypothetical protein (RefSeq), at 1,142,469 to 1,143,170
_0972
Shewana3_0973: Shewana3_0973 - endonuclease/exonuclease/phosphatase (RefSeq), at 1,143,851 to 1,144,984
_0973
Shewana3_0974: Shewana3_0974 - hypothetical protein (RefSeq), at 1,144,996 to 1,145,811
_0974
Shewana3_0975: Shewana3_0975 - hypothetical protein (RefSeq), at 1,145,808 to 1,146,689
_0975
Position (kb)
1143
1144
1145 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1143.021 kb on - strand, within Shewana3_0972 at 1143.021 kb on - strand, within Shewana3_0972 at 1143.122 kb on - strand at 1143.365 kb on - strand at 1144.072 kb on - strand, within Shewana3_0973 at 1144.072 kb on - strand, within Shewana3_0973 at 1144.292 kb on - strand, within Shewana3_0973 at 1144.369 kb on - strand, within Shewana3_0973 at 1144.381 kb on + strand, within Shewana3_0973 at 1144.524 kb on + strand, within Shewana3_0973 at 1144.811 kb on + strand, within Shewana3_0973 at 1144.821 kb on + strand, within Shewana3_0973 at 1144.893 kb on + strand at 1144.901 kb on - strand at 1144.933 kb on + strand at 1145.120 kb on + strand, within Shewana3_0974 at 1145.173 kb on + strand, within Shewana3_0974 at 1145.392 kb on + strand, within Shewana3_0974 at 1145.665 kb on - strand, within Shewana3_0974 at 1145.833 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,143,021 - Shewana3_0972 0.79 -4.4 1,143,021 - Shewana3_0972 0.79 -4.4 1,143,122 - -3.8 1,143,365 - -4.9 1,144,072 - Shewana3_0973 0.19 -2.1 1,144,072 - Shewana3_0973 0.19 -1.3 1,144,292 - Shewana3_0973 0.39 +0.3 1,144,369 - Shewana3_0973 0.46 -0.7 1,144,381 + Shewana3_0973 0.47 -1.0 1,144,524 + Shewana3_0973 0.59 +0.3 1,144,811 + Shewana3_0973 0.85 -1.8 1,144,821 + Shewana3_0973 0.86 -4.3 1,144,893 + -0.1 1,144,901 - +1.1 1,144,933 + +0.4 1,145,120 + Shewana3_0974 0.15 +0.2 1,145,173 + Shewana3_0974 0.22 +0.1 1,145,392 + Shewana3_0974 0.49 +0.6 1,145,665 - Shewana3_0974 0.82 +0.4 1,145,833 + -1.4
Or see this region's nucleotide sequence